WRKY9 transcription factor regulates cytochrome P450 genes CYP94B3 and CYP86B1, leading to increased root suberin and salt tolerance in Arabidopsis

Salinity affects crop productivity worldwide and mangroves growing under high salinity exhibit adaptations such as enhanced root apoplastic barrier to survive under such conditions. We have identified two cytochrome P450 family genes, AoCYP94B3 and AoCYP86B1 from the mangrove tree Avicennia officina...

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Published in:Physiologia plantarum Vol. 172; no. 3; pp. 1673 - 1687
Main Authors: Krishnamurthy, Pannaga, Vishal, Bhushan, Bhal, Amrit, Kumar, Prakash P.
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Publishing Ltd 01.07.2021
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Subjects:
Acids
Adaptation
Arabidopsis
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Avicennia officinalis
Chromatin
chromatin immunoprecipitation
Crop production
Cytochrome
cytochrome P-450
Cytochrome P-450 Enzyme System - genetics
Cytochrome P-450 Enzyme System - metabolism
Cytochrome P450
Cytochromes P450
Dicarboxylic acids
Electrophoretic mobility
Endoplasmic reticulum
gas chromatography
Gene Expression Regulation, Plant
Genes
heterologous gene expression
Hydroxy acids
Immunoprecipitation
Lipids
Mangrove trees
Mangroves
mangroves (trees)
Mass spectrometry
Mass spectroscopy
Monomers
Mutants
phenotype
Phenotypes
Plant Roots - genetics
Plant Roots - metabolism
Plants, Genetically Modified - metabolism
Roots
Salinity
Salinity effects
Salinity tolerance
Salt tolerance
Salt Tolerance - genetics
Seedlings
Shoots
suberin
Transcription Factors
ISSN:0031-9317, 1399-3054, 1399-3054
Online Access:Get full text
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Summary:Salinity affects crop productivity worldwide and mangroves growing under high salinity exhibit adaptations such as enhanced root apoplastic barrier to survive under such conditions. We have identified two cytochrome P450 family genes, AoCYP94B3 and AoCYP86B1 from the mangrove tree Avicennia officinalis and characterized them using atcyp94b3 and atcyp86b1, which are mutants of their putative Arabidopsis orthologs and the corresponding complemented lines with A. officinalis genes. CYP94B3 and CYP86B1 transcripts were induced upon salt treatment in the roots of both A. officinalis and Arabidopsis. Both AoCYP94B3 and AoCYP86B1 were localized to the endoplasmic reticulum. Heterologous expression of 35S::AoCYP94B3 and 35S::AoCYP86B1 in their respective Arabidopsis mutants (atcyp94b3 and atcyp86b1) increased the salt tolerance of the transgenic seedlings by reducing the amount of Na+ accumulation in the shoots. Moreover, the reduced root suberin phenotype of atcyp94b3 was rescued in the 35S::AoCYP94B3;atcyp94b3 transgenic Arabidopsis seedlings. Gas‐chromatography and mass spectrometry analyses showed that the amount of suberin monomers (C‐16 ω‐hydroxy acids, C‐16 α, ω‐dicarboxylic acids and C‐20 eicosanol) were increased in the roots of 35S::AoCYP94B3;atcyp94b3 Arabidopsis seedlings. Using chromatin immunoprecipitation and electrophoretic mobility shift assays, we identified AtWRKY9 as the upstream regulator of AtCYP94B3 and AtCYP86B1 in Arabidopsis. In addition, atwrky9 showed suppressed expression of AtCYP94B3 and AtCYP86B1 transcripts, and reduced suberin in the roots. These results show that AtWRKY9 controls suberin deposition by regulating AtCYP94B3 and AtCYP86B1, leading to salt tolerance. Our data can be used for generating salt‐tolerant crop plants in the future.
Bibliography:Funding information
National Water Agency; National Research Foundation, Singapore
Edited by B. Huang
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ISSN:0031-9317
1399-3054
1399-3054
DOI:10.1111/ppl.13371