Molecular aspects of mucin biosynthesis and mucus formation in the bovine cervix during the periestrous period
Mucus within the cervical canal represents a hormonally regulated barrier that reconciles the need to exclude the vaginal microflora from the uterus during progesterone dominance, while permitting sperm transport at estrus. Its characteristics change during the estrous cycle to facilitate these comp...
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| Veröffentlicht in: | Physiological genomics Jg. 44; H. 24; S. 1165 |
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| Sprache: | Englisch |
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18.12.2012
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| ISSN: | 1531-2267, 1531-2267 |
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| Abstract | Mucus within the cervical canal represents a hormonally regulated barrier that reconciles the need to exclude the vaginal microflora from the uterus during progesterone dominance, while permitting sperm transport at estrus. Its characteristics change during the estrous cycle to facilitate these competing functional requirements. Hydrated mucin glycoproteins synthesized by the endocervical epithelium form the molecular scaffold of this mucus. This study uses the bovine cervix as a model to examine functional groups of genes related to mucin biosynthesis and mucus production over the periestrous period when functional changes in cervical barrier function are most prominent. Cervical tissue samples were collected from 30 estrus synchronized beef heifers. Animals were slaughtered in groups starting 12 h after the withdrawal of intravaginal progesterone releasing devices (controlled internal drug releases) until 7 days postonset of estrus (luteal phase). Subsequent groupings represented proestrus, early estrus, late estrus, metestrus, and finally the early luteal phase. Tissues were submitted to next generation RNA-seq transcriptome analysis. We identified 114 genes associated with biosynthesis and intracellular transport of mucins, and postsecretory modifications of cervical; 53 of these genes showed at least a twofold change in one or more experimental group in relation to onset of estrus, and the differences between groups were significant (P < 0.05). The majority of these genes showed the greatest alteration in their expression in the 48 h postestrus and luteal phase groups. |
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| AbstractList | Mucus within the cervical canal represents a hormonally regulated barrier that reconciles the need to exclude the vaginal microflora from the uterus during progesterone dominance, while permitting sperm transport at estrus. Its characteristics change during the estrous cycle to facilitate these competing functional requirements. Hydrated mucin glycoproteins synthesized by the endocervical epithelium form the molecular scaffold of this mucus. This study uses the bovine cervix as a model to examine functional groups of genes related to mucin biosynthesis and mucus production over the periestrous period when functional changes in cervical barrier function are most prominent. Cervical tissue samples were collected from 30 estrus synchronized beef heifers. Animals were slaughtered in groups starting 12 h after the withdrawal of intravaginal progesterone releasing devices (controlled internal drug releases) until 7 days postonset of estrus (luteal phase). Subsequent groupings represented proestrus, early estrus, late estrus, metestrus, and finally the early luteal phase. Tissues were submitted to next generation RNA-seq transcriptome analysis. We identified 114 genes associated with biosynthesis and intracellular transport of mucins, and postsecretory modifications of cervical; 53 of these genes showed at least a twofold change in one or more experimental group in relation to onset of estrus, and the differences between groups were significant (P < 0.05). The majority of these genes showed the greatest alteration in their expression in the 48 h postestrus and luteal phase groups.Mucus within the cervical canal represents a hormonally regulated barrier that reconciles the need to exclude the vaginal microflora from the uterus during progesterone dominance, while permitting sperm transport at estrus. Its characteristics change during the estrous cycle to facilitate these competing functional requirements. Hydrated mucin glycoproteins synthesized by the endocervical epithelium form the molecular scaffold of this mucus. This study uses the bovine cervix as a model to examine functional groups of genes related to mucin biosynthesis and mucus production over the periestrous period when functional changes in cervical barrier function are most prominent. Cervical tissue samples were collected from 30 estrus synchronized beef heifers. Animals were slaughtered in groups starting 12 h after the withdrawal of intravaginal progesterone releasing devices (controlled internal drug releases) until 7 days postonset of estrus (luteal phase). Subsequent groupings represented proestrus, early estrus, late estrus, metestrus, and finally the early luteal phase. Tissues were submitted to next generation RNA-seq transcriptome analysis. We identified 114 genes associated with biosynthesis and intracellular transport of mucins, and postsecretory modifications of cervical; 53 of these genes showed at least a twofold change in one or more experimental group in relation to onset of estrus, and the differences between groups were significant (P < 0.05). The majority of these genes showed the greatest alteration in their expression in the 48 h postestrus and luteal phase groups. Mucus within the cervical canal represents a hormonally regulated barrier that reconciles the need to exclude the vaginal microflora from the uterus during progesterone dominance, while permitting sperm transport at estrus. Its characteristics change during the estrous cycle to facilitate these competing functional requirements. Hydrated mucin glycoproteins synthesized by the endocervical epithelium form the molecular scaffold of this mucus. This study uses the bovine cervix as a model to examine functional groups of genes related to mucin biosynthesis and mucus production over the periestrous period when functional changes in cervical barrier function are most prominent. Cervical tissue samples were collected from 30 estrus synchronized beef heifers. Animals were slaughtered in groups starting 12 h after the withdrawal of intravaginal progesterone releasing devices (controlled internal drug releases) until 7 days postonset of estrus (luteal phase). Subsequent groupings represented proestrus, early estrus, late estrus, metestrus, and finally the early luteal phase. Tissues were submitted to next generation RNA-seq transcriptome analysis. We identified 114 genes associated with biosynthesis and intracellular transport of mucins, and postsecretory modifications of cervical; 53 of these genes showed at least a twofold change in one or more experimental group in relation to onset of estrus, and the differences between groups were significant (P < 0.05). The majority of these genes showed the greatest alteration in their expression in the 48 h postestrus and luteal phase groups. |
| Author | Pluta, Katarzyna McGettigan, Paul A Evans, Alexander C O Reid, Colm J Browne, John A Loftus, Brendan J Tharmalingam, Tharmala Irwin, Jane A Corfield, Anthony Carrington, Stephen D Baird, Alan |
| Author_xml | – sequence: 1 givenname: Katarzyna surname: Pluta fullname: Pluta, Katarzyna email: kasia.a.pluta@gmail.com organization: Veterinary Sciences Centre, University College Dublin, Belfield, Dublin, Ireland. kasia.a.pluta@gmail.com – sequence: 2 givenname: Paul A surname: McGettigan fullname: McGettigan, Paul A – sequence: 3 givenname: Colm J surname: Reid fullname: Reid, Colm J – sequence: 4 givenname: John A surname: Browne fullname: Browne, John A – sequence: 5 givenname: Jane A surname: Irwin fullname: Irwin, Jane A – sequence: 6 givenname: Tharmala surname: Tharmalingam fullname: Tharmalingam, Tharmala – sequence: 7 givenname: Anthony surname: Corfield fullname: Corfield, Anthony – sequence: 8 givenname: Alan surname: Baird fullname: Baird, Alan – sequence: 9 givenname: Brendan J surname: Loftus fullname: Loftus, Brendan J – sequence: 10 givenname: Alexander C O surname: Evans fullname: Evans, Alexander C O – sequence: 11 givenname: Stephen D surname: Carrington fullname: Carrington, Stephen D |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/23092952$$D View this record in MEDLINE/PubMed |
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| SubjectTerms | Animals Biological Transport Calcium - metabolism Cattle Cervix Uteri - metabolism Epithelial Cells - metabolism Epithelium - metabolism Estrous Cycle - metabolism Female Gene Expression Regulation Homeostasis - genetics Hormones - metabolism Intracellular Space - metabolism Mucins - biosynthesis Mucins - genetics Mucus - metabolism Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism Transcription Factors - metabolism |
| Title | Molecular aspects of mucin biosynthesis and mucus formation in the bovine cervix during the periestrous period |
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