CXCR6 regulates localization of tissue-resident memory CD8 T cells to the airways

Resident memory T cells (T cells) are an important first-line defense against respiratory pathogens, but the unique contributions of lung T cell populations to protective immunity and the factors that govern their localization to different compartments of the lung are not well understood. Here, we s...

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Vydané v:The Journal of experimental medicine Ročník 216; číslo 12; s. 2748
Hlavní autori: Wein, Alexander N, McMaster, Sean R, Takamura, Shiki, Dunbar, Paul R, Cartwright, Emily K, Hayward, Sarah L, McManus, Daniel T, Shimaoka, Takeshi, Ueha, Satoshi, Tsukui, Tatsuya, Masumoto, Tomoko, Kurachi, Makoto, Matsushima, Kouji, Kohlmeier, Jacob E
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 02.12.2019
ISSN:1540-9538, 1540-9538
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Abstract Resident memory T cells (T cells) are an important first-line defense against respiratory pathogens, but the unique contributions of lung T cell populations to protective immunity and the factors that govern their localization to different compartments of the lung are not well understood. Here, we show that airway and interstitial T cells have distinct effector functions and that CXCR6 controls the partitioning of T cells within the lung by recruiting CD8 T cells to the airways. The absence of CXCR6 significantly decreases airway CD8 T cells due to altered trafficking of CXCR6 cells within the lung, and not decreased survival in the airways. CXCL16, the ligand for CXCR6, is localized primarily at the respiratory epithelium, and mice lacking CXCL16 also had decreased CD8 T cells in the airways. Finally, blocking CXCL16 inhibited the steady-state maintenance of airway T cells. Thus, the CXCR6/CXCL16 signaling axis controls the localization of T cells to different compartments of the lung and maintains airway T cells.
AbstractList Resident memory T cells (TRM cells) are an important first-line defense against respiratory pathogens, but the unique contributions of lung TRM cell populations to protective immunity and the factors that govern their localization to different compartments of the lung are not well understood. Here, we show that airway and interstitial TRM cells have distinct effector functions and that CXCR6 controls the partitioning of TRM cells within the lung by recruiting CD8 TRM cells to the airways. The absence of CXCR6 significantly decreases airway CD8 TRM cells due to altered trafficking of CXCR6-/- cells within the lung, and not decreased survival in the airways. CXCL16, the ligand for CXCR6, is localized primarily at the respiratory epithelium, and mice lacking CXCL16 also had decreased CD8 TRM cells in the airways. Finally, blocking CXCL16 inhibited the steady-state maintenance of airway TRM cells. Thus, the CXCR6/CXCL16 signaling axis controls the localization of TRM cells to different compartments of the lung and maintains airway TRM cells.Resident memory T cells (TRM cells) are an important first-line defense against respiratory pathogens, but the unique contributions of lung TRM cell populations to protective immunity and the factors that govern their localization to different compartments of the lung are not well understood. Here, we show that airway and interstitial TRM cells have distinct effector functions and that CXCR6 controls the partitioning of TRM cells within the lung by recruiting CD8 TRM cells to the airways. The absence of CXCR6 significantly decreases airway CD8 TRM cells due to altered trafficking of CXCR6-/- cells within the lung, and not decreased survival in the airways. CXCL16, the ligand for CXCR6, is localized primarily at the respiratory epithelium, and mice lacking CXCL16 also had decreased CD8 TRM cells in the airways. Finally, blocking CXCL16 inhibited the steady-state maintenance of airway TRM cells. Thus, the CXCR6/CXCL16 signaling axis controls the localization of TRM cells to different compartments of the lung and maintains airway TRM cells.
Resident memory T cells (T cells) are an important first-line defense against respiratory pathogens, but the unique contributions of lung T cell populations to protective immunity and the factors that govern their localization to different compartments of the lung are not well understood. Here, we show that airway and interstitial T cells have distinct effector functions and that CXCR6 controls the partitioning of T cells within the lung by recruiting CD8 T cells to the airways. The absence of CXCR6 significantly decreases airway CD8 T cells due to altered trafficking of CXCR6 cells within the lung, and not decreased survival in the airways. CXCL16, the ligand for CXCR6, is localized primarily at the respiratory epithelium, and mice lacking CXCL16 also had decreased CD8 T cells in the airways. Finally, blocking CXCL16 inhibited the steady-state maintenance of airway T cells. Thus, the CXCR6/CXCL16 signaling axis controls the localization of T cells to different compartments of the lung and maintains airway T cells.
Author Masumoto, Tomoko
McManus, Daniel T
Dunbar, Paul R
Hayward, Sarah L
Takamura, Shiki
Wein, Alexander N
McMaster, Sean R
Tsukui, Tatsuya
Matsushima, Kouji
Ueha, Satoshi
Cartwright, Emily K
Shimaoka, Takeshi
Kohlmeier, Jacob E
Kurachi, Makoto
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  orcidid: 0000-0002-8813-3523
  surname: Wein
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  organization: Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA
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  organization: Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA
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  orcidid: 0000-0002-9082-6636
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  organization: Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA
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  orcidid: 0000-0001-7052-5556
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  surname: Shimaoka
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  organization: Division of Molecular Regulation of Inflammatory and Immune Diseases, Research Institute for Biomedical Sciences, Tokyo University of Science, Noda, Chiba, Japan
– sequence: 9
  givenname: Satoshi
  surname: Ueha
  fullname: Ueha, Satoshi
  organization: Division of Molecular Regulation of Inflammatory and Immune Diseases, Research Institute for Biomedical Sciences, Tokyo University of Science, Noda, Chiba, Japan
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  organization: Department of Immunology, Kindai University Faculty of Medicine, Osaka-Sayama, Osaka, Japan
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  organization: Department of Microbiology and Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA
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  email: jkohlmeier@emory.edu
  organization: Emory-UGA Center of Excellence for Influenza Research and Surveillance, Atlanta, GA
BackLink https://www.ncbi.nlm.nih.gov/pubmed/31558615$$D View this record in MEDLINE/PubMed
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  year: 2019
  text: 2019-12-02
  day: 02
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PublicationTitle The Journal of experimental medicine
PublicationTitleAlternate J Exp Med
PublicationYear 2019
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Snippet Resident memory T cells (T cells) are an important first-line defense against respiratory pathogens, but the unique contributions of lung T cell populations to...
Resident memory T cells (TRM cells) are an important first-line defense against respiratory pathogens, but the unique contributions of lung TRM cell...
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Title CXCR6 regulates localization of tissue-resident memory CD8 T cells to the airways
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Volume 216
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