Human Umbilical Vein Endothelial Cells as a Versatile Cellular Model System in Diverse Experimental Paradigms: An Ultrastructural Perspective
Human umbilical vein endothelial cells (HUVECs) are primary cells isolated from the vein of an umbilical cord, extensively used in cardiovascular studies and medical research. These cells, retaining the characteristics of endothelial cells in vivo, serve as a valuable cellular model system for under...
Uloženo v:
| Vydáno v: | Microscopy and microanalysis Ročník 30; číslo 3; s. 419 |
|---|---|
| Hlavní autoři: | , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
England
31.05.2024
|
| Témata: | |
| ISSN: | 1435-8115, 1435-8115 |
| On-line přístup: | Zjistit podrobnosti o přístupu |
| Tagy: |
Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
|
| Abstract | Human umbilical vein endothelial cells (HUVECs) are primary cells isolated from the vein of an umbilical cord, extensively used in cardiovascular studies and medical research. These cells, retaining the characteristics of endothelial cells in vivo, serve as a valuable cellular model system for understanding vascular biology, endothelial dysfunction, pathophysiology of diseases such as atherosclerosis, and responses to different drugs or treatments. Transmission electron microscopy (TEM) has been a cornerstone in revealing the detailed architecture of multiple cellular model systems including HUVECs, allowing researchers to visualize subcellular organelles, membrane structures, and cytoskeletal elements. Among them, the endoplasmic reticulum, Golgi apparatus, mitochondria, and nucleus can be meticulously examined to recognize alterations indicative of cellular responses to various stimuli. Importantly, Weibel-Palade bodies are characteristic secretory organelles found in HUVECs, which can be easily distinguished in the TEM. These distinctive structures also dynamically react to different factors through regulated exocytosis, resulting in complete or selective release of their contents. This detailed review summarizes the ultrastructural features of HUVECs and highlights the utility of TEM as a pivotal tool for analyzing HUVECs in diverse research frameworks, contributing valuable insights into the comprehension of HUVEC behavior and enriching our knowledge into the complexity of vascular biology. |
|---|---|
| AbstractList | Human umbilical vein endothelial cells (HUVECs) are primary cells isolated from the vein of an umbilical cord, extensively used in cardiovascular studies and medical research. These cells, retaining the characteristics of endothelial cells in vivo, serve as a valuable cellular model system for understanding vascular biology, endothelial dysfunction, pathophysiology of diseases such as atherosclerosis, and responses to different drugs or treatments. Transmission electron microscopy (TEM) has been a cornerstone in revealing the detailed architecture of multiple cellular model systems including HUVECs, allowing researchers to visualize subcellular organelles, membrane structures, and cytoskeletal elements. Among them, the endoplasmic reticulum, Golgi apparatus, mitochondria, and nucleus can be meticulously examined to recognize alterations indicative of cellular responses to various stimuli. Importantly, Weibel-Palade bodies are characteristic secretory organelles found in HUVECs, which can be easily distinguished in the TEM. These distinctive structures also dynamically react to different factors through regulated exocytosis, resulting in complete or selective release of their contents. This detailed review summarizes the ultrastructural features of HUVECs and highlights the utility of TEM as a pivotal tool for analyzing HUVECs in diverse research frameworks, contributing valuable insights into the comprehension of HUVEC behavior and enriching our knowledge into the complexity of vascular biology.Human umbilical vein endothelial cells (HUVECs) are primary cells isolated from the vein of an umbilical cord, extensively used in cardiovascular studies and medical research. These cells, retaining the characteristics of endothelial cells in vivo, serve as a valuable cellular model system for understanding vascular biology, endothelial dysfunction, pathophysiology of diseases such as atherosclerosis, and responses to different drugs or treatments. Transmission electron microscopy (TEM) has been a cornerstone in revealing the detailed architecture of multiple cellular model systems including HUVECs, allowing researchers to visualize subcellular organelles, membrane structures, and cytoskeletal elements. Among them, the endoplasmic reticulum, Golgi apparatus, mitochondria, and nucleus can be meticulously examined to recognize alterations indicative of cellular responses to various stimuli. Importantly, Weibel-Palade bodies are characteristic secretory organelles found in HUVECs, which can be easily distinguished in the TEM. These distinctive structures also dynamically react to different factors through regulated exocytosis, resulting in complete or selective release of their contents. This detailed review summarizes the ultrastructural features of HUVECs and highlights the utility of TEM as a pivotal tool for analyzing HUVECs in diverse research frameworks, contributing valuable insights into the comprehension of HUVEC behavior and enriching our knowledge into the complexity of vascular biology. Human umbilical vein endothelial cells (HUVECs) are primary cells isolated from the vein of an umbilical cord, extensively used in cardiovascular studies and medical research. These cells, retaining the characteristics of endothelial cells in vivo, serve as a valuable cellular model system for understanding vascular biology, endothelial dysfunction, pathophysiology of diseases such as atherosclerosis, and responses to different drugs or treatments. Transmission electron microscopy (TEM) has been a cornerstone in revealing the detailed architecture of multiple cellular model systems including HUVECs, allowing researchers to visualize subcellular organelles, membrane structures, and cytoskeletal elements. Among them, the endoplasmic reticulum, Golgi apparatus, mitochondria, and nucleus can be meticulously examined to recognize alterations indicative of cellular responses to various stimuli. Importantly, Weibel-Palade bodies are characteristic secretory organelles found in HUVECs, which can be easily distinguished in the TEM. These distinctive structures also dynamically react to different factors through regulated exocytosis, resulting in complete or selective release of their contents. This detailed review summarizes the ultrastructural features of HUVECs and highlights the utility of TEM as a pivotal tool for analyzing HUVECs in diverse research frameworks, contributing valuable insights into the comprehension of HUVEC behavior and enriching our knowledge into the complexity of vascular biology. |
| Author | Simora, Veronika Fialkova, Veronika Kuzelova, Lenka Duranova, Hana Borotova, Petra |
| Author_xml | – sequence: 1 givenname: Hana orcidid: 0000-0002-7274-6210 surname: Duranova fullname: Duranova, Hana organization: AgroBioTech Research Centre, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic – sequence: 2 givenname: Lenka surname: Kuzelova fullname: Kuzelova, Lenka organization: Faculty of Biotechnology and Food Sciences, Institute of Biotechnology, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic – sequence: 3 givenname: Petra surname: Borotova fullname: Borotova, Petra organization: AgroBioTech Research Centre, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic – sequence: 4 givenname: Veronika surname: Simora fullname: Simora, Veronika organization: AgroBioTech Research Centre, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic – sequence: 5 givenname: Veronika orcidid: 0000-0002-3361-0724 surname: Fialkova fullname: Fialkova, Veronika organization: AgroBioTech Research Centre, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/38817111$$D View this record in MEDLINE/PubMed |
| BookMark | eNpNUE1LAzEQDVKxH3ryLjl6qU02293UW6nVChUFrdcym53VSLJbk6xY_4P_2agVhIEZ3rz3hjd90qmbGgk55uyMs4kYWbCj5gOQpXKP9HgqxkPJ-bjzb-6SvvcvjDHB8uyAdIWUPOec98jnorVQ05UttNEKDH1EXdN5XTbhGY2OwAyN8RRixZ3zELTBH7A14OhNU6Kh91sf0NKovNBvkYR0_r5Bpy3WIVrcgYNSP1l_TqfxlgkOfHCtCq373kbBBlWIykOyX4HxeLTrA7K6nD_MFsPl7dX1bLocKiFFGMpcpKWSSVmAqLjKiyyvJAiUKsMEKpGlwBIF42yMeSJEKdJKcpQFq3KhOJbJgJz--m5c89qiD2urvYqZoMam9WvBMpFmPOWTSD3ZUdvCYrnexFTgtuu_FyZf6DV3sQ |
| CitedBy_id | crossref_primary_10_1111_aor_14950 crossref_primary_10_1063_5_0275985 crossref_primary_10_1248_bpb_b25_00128 crossref_primary_10_1002_jat_4885 crossref_primary_10_1016_j_csbj_2025_06_023 crossref_primary_10_1016_j_heliyon_2025_e43397 crossref_primary_10_1002_advs_202416904 crossref_primary_10_3390_cimb47090685 crossref_primary_10_3390_nu16172986 crossref_primary_10_1177_00368504251366369 crossref_primary_10_3892_mmr_2025_13596 crossref_primary_10_1007_s12010_025_05312_2 crossref_primary_10_3390_ijms26189217 crossref_primary_10_1016_j_exer_2024_110185 crossref_primary_10_1016_j_ijpharm_2024_124720 crossref_primary_10_2147_JIR_S477554 crossref_primary_10_1016_j_atherosclerosis_2025_119119 crossref_primary_10_1093_rheumatology_keaf025 |
| ContentType | Journal Article |
| Copyright | The Author(s) 2024. Published by Oxford University Press on behalf of the Microscopy Society of America. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com. |
| Copyright_xml | – notice: The Author(s) 2024. Published by Oxford University Press on behalf of the Microscopy Society of America. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com. |
| DBID | NPM 7X8 |
| DOI | 10.1093/mam/ozae048 |
| DatabaseName | PubMed MEDLINE - Academic |
| DatabaseTitle | PubMed MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic PubMed |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 1435-8115 |
| ExternalDocumentID | 38817111 |
| Genre | Journal Article |
| GroupedDBID | --- -E. .FH 0E1 0R~ 123 29M 4.4 53G 5VS 74X 74Y 7RV 7X7 7~V 9M5 AAAZR AACJH AAGFV AAKTX AAPXW AARAB AAUAY AAUKB ABBXD ABDFA ABITZ ABJNI ABKKG ABPTD ABQTM ABROB ABWCF ABWST ABZCX ACBEK ACBMC ACGFS ACIMK ACIWK ACPRK ACUFI ACUIJ ACYZP ACZBM ACZUX ACZWT ADAZD ADBBV ADCGK ADFEC ADKIL ADQBN ADRDM ADVEK AEBAK AEMTW AENEX AEYYC AFFUJ AFGWE AFLOS AFLVW AFRAH AFUTZ AGABE AGBYD AGJUD AHIPN AHLTW AHMBA AISIE AJ7 AJPFC AJQAS ALMA_UNASSIGNED_HOLDINGS ALWZO AQJOH ARABE ARAPS ATGXG ATUCA AZGZS BBLKV BBNVY BENPR BGHMG BHPHI BLZWO BMAJL C0O CJCSC CS3 DU5 EBS F5P HCIFZ HG- HST HZ~ I.6 IH6 IS6 I~P J36 J38 J3A JKPOH KOP L98 M-V M4Y M7P NAPCQ NIKVX NPM O9- OVD OYBOY PYCCK RAMDC RCA RNS ROL RR0 S6- S6U SDH SY4 T9M TEORI UT1 UU6 VUG WFFJZ WQ3 WXU WYP 7X8 8FE 8FG 8FH 8R4 8R5 ABPQP AFKRA AHGBF AJBYB AJNCP BGLVJ BKEYQ BPHCQ BVXVI EX3 FYUFA H13 LK8 P62 PQQKQ PROAC PSQYO Q2X UKHRP WOW |
| ID | FETCH-LOGICAL-c383t-8734dc82dba3f1c7b67f8a3e8c6e2af364a02ca565e7233d34f81e8b0f73c1ed2 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 17 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=001235361000001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1435-8115 |
| IngestDate | Sun Sep 28 11:27:47 EDT 2025 Wed Feb 19 02:01:44 EST 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 3 |
| Keywords | Weibel–Palade bodies endothelial cells umbilical cord vein transmission electron microscopy ultrastructural characteristics |
| Language | English |
| License | The Author(s) 2024. Published by Oxford University Press on behalf of the Microscopy Society of America. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c383t-8734dc82dba3f1c7b67f8a3e8c6e2af364a02ca565e7233d34f81e8b0f73c1ed2 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 ObjectType-Review-3 content type line 23 |
| ORCID | 0000-0002-7274-6210 0000-0002-3361-0724 |
| PMID | 38817111 |
| PQID | 3063461419 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_3063461419 pubmed_primary_38817111 |
| PublicationCentury | 2000 |
| PublicationDate | 2024-05-31 |
| PublicationDateYYYYMMDD | 2024-05-31 |
| PublicationDate_xml | – month: 05 year: 2024 text: 2024-05-31 day: 31 |
| PublicationDecade | 2020 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England |
| PublicationTitle | Microscopy and microanalysis |
| PublicationTitleAlternate | Microsc Microanal |
| PublicationYear | 2024 |
| SSID | ssj0003076 |
| Score | 2.4553008 |
| SecondaryResourceType | review_article |
| Snippet | Human umbilical vein endothelial cells (HUVECs) are primary cells isolated from the vein of an umbilical cord, extensively used in cardiovascular studies and... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 419 |
| Title | Human Umbilical Vein Endothelial Cells as a Versatile Cellular Model System in Diverse Experimental Paradigms: An Ultrastructural Perspective |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/38817111 https://www.proquest.com/docview/3063461419 |
| Volume | 30 |
| WOSCitedRecordID | wos001235361000001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LS8NAEF7UKnjx_agvVvAammQ3ydaLFG3xoKUHK72V2ZcU0rQ2VdD_4H92NkmpF0EQQg6BJWFnMvvt7Mz3EXLFGW7-I-F7SjS5x1XiexDE0pNWQQSOs84W7PoPSbcrBoNmr0q45VVZ5SImFoFaT5TLkTcQ2jKOa0nQvJm-ek41yp2uVhIaq6TGEMq4kq5ksGQLR_8tu4tY5AmEPlV_Hm7iG2MYNyafYHwufseWxRrT2f7v1-2QrQpd0lbpDrtkxWR7ZKPUm_zYJ19Fyp72x64iFo1Dn80oo-1MuzasFD2R3po0zSngRV0mDc2WmuKhq1alTjgtpSXJOcWRd0VRh6HtHzIBtAcz0KOXcX5NW_iudD6DkqTWEXzQ3rK584D0O-2n23uv0mPwFO5j5xg4GddKhFoCs4FKZJxYAcwIFZsQLIs5GhdNHEcmCRnTjFsRGCF9mzAVGB0ekrVskpljQqVlPNLWB8kFF0pKHamgyX3gNgTfhzq5XMzzEP3dHWJAZiZv-XA503VyVBprOC2JOYZMiCDB4H3yh9GnZDNEfFIWApyRmsW_3ZyTdfU-H-Wzi8KR8N7tPX4DxWjWiw |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Human+Umbilical+Vein+Endothelial+Cells+as+a+Versatile+Cellular+Model+System+in+Diverse+Experimental+Paradigms%3A+An+Ultrastructural+Perspective&rft.jtitle=Microscopy+and+microanalysis&rft.au=Duranova%2C+Hana&rft.au=Kuzelova%2C+Lenka&rft.au=Borotova%2C+Petra&rft.au=Simora%2C+Veronika&rft.date=2024-05-31&rft.issn=1435-8115&rft.eissn=1435-8115&rft.volume=30&rft.issue=3&rft.spage=419&rft_id=info:doi/10.1093%2Fmam%2Fozae048&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1435-8115&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1435-8115&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1435-8115&client=summon |