The Function of Chitinases CmCH1 and CmCH10 in the Interaction of Coniothyrium minitans and Sclerotinia sclerotiorum

Sclerotinia sclerotiorum, a devastating phytopathogenic fungus with global distribution, exhibits a broad host range encompassing over 700 plant species. Sclerotinia stem rot caused by this pathogen poses a significant threat to sustainable oilseed rape production. Coniothyrium minitans, a mycoparas...

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Published in:International journal of molecular sciences Vol. 26; no. 17; p. 8706
Main Authors: Wang, Haixuan, Zhao, Huizhang, Zhu, Zihang, Lin, Yang, Xie, Jiatao, Cheng, Jiasen, Jiang, Daohong, Fu, Yanping
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 06.09.2025
Subjects:
Ascomycota - enzymology
Ascomycota - genetics
Ascomycota - pathogenicity
Ascomycota - physiology
Biological control
Cell Wall - metabolism
Chitinase
Chitinases - genetics
Chitinases - metabolism
Disease
Enzymes
Fungal Proteins - genetics
Fungal Proteins - metabolism
Fungi
Gene Expression Regulation, Fungal
Genes
Genetic aspects
Pathogens
Peptides
Pests
Plant Diseases - microbiology
China
Sclerotinia sclerotiorum
chitinase
Coniothyrium minitans
gene function
ISSN:1422-0067, 1661-6596, 1422-0067
Online Access:Get full text
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Summary:Sclerotinia sclerotiorum, a devastating phytopathogenic fungus with global distribution, exhibits a broad host range encompassing over 700 plant species. Sclerotinia stem rot caused by this pathogen poses a significant threat to sustainable oilseed rape production. Coniothyrium minitans, a mycoparasite of S. sclerotiorum, is a promising biological control agent against this devastating disease. C. minitans-based formulations have been commercially developed for field application. A transcriptomic analysis revealed significant upregulation of the chitinase-encoding gene CmCH1 in C. minitans during interaction with S. sclerotiorum. Knockout of either CmCH1 or another chitinase-encoding gene CmCH10 in C. minitans did not markedly affect the mycelial growth, development, and parasitism of S. sclerotiorum. However, knockout CmCH1 and CmCH10 simultaneously resulted in reduced growth rate, impaired protoplast release, enhanced cell wall integrity, and diminished mycoparasitic capability. These results indicate that CmCH1 and CmCH10 collectively influence remodeling of the cell wall in C. minitans and its mycoparasitic activity.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms26178706