Prime editing in plants and mammalian cells: Mechanism, achievements, limitations, and future prospects

Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR‐associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas‐derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have been...

Full description

Saved in:
Bibliographic Details
Published in:BioEssays Vol. 44; no. 9; pp. e2200032 - n/a
Main Authors: Hillary, V. Edwin, Ceasar, S. Antony
Format: Journal Article
Language:English
Published: Cambridge Wiley Subscription Services, Inc 01.09.2022
Subjects:
base editing
Cas9 H840 nickase
CRISPR
CRISPR/Cas
Editing
gene editing
genome
Genomes
Mammalian cells
Mammals
Nuclease
pegRNA
plants
prime editing
Recombinase
recombinases
researchers
ISSN:0265-9247, 1521-1878, 1521-1878
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR‐associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas‐derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have been introduced for engineering the genomes of diverse organisms. The recently introduced prime editing system offers precise editing without many off‐target effects than traditional CRISPR‐based systems. Many researchers have successfully applied this gene‐editing toolbox in diverse systems for various genome‐editing applications. This review presents the mechanism of prime editing and summarizes the details of the prime editing system applied in plants and mammalian cells for precise genome editing. We also discuss the advantages, limitations, and potential future applications of prime editing in these systems. This review enables the researcher to gain knowledge on prime editing tools and their potential applications in plants and mammalian cells. Prime editing is a search‐and‐replace genome editing system, which mediates all possible 12 base‐to‐base conversions without DSBs and donor templates at a targeted sequence. We describe how this prime editing system helps in improving genetic studies in plants and mammalian cells with their mechanism, achievements, limitations, and future prospects.
AbstractList Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR-associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas-derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have been introduced for engineering the genomes of diverse organisms. The recently introduced prime editing system offers precise editing without many off-target effects than traditional CRISPR-based systems. Many researchers have successfully applied this gene-editing toolbox in diverse systems for various genome-editing applications. This review presents the mechanism of prime editing and summarizes the details of the prime editing system applied in plants and mammalian cells for precise genome editing. We also discuss the advantages, limitations, and potential future applications of prime editing in these systems. This review enables the researcher to gain knowledge on prime editing tools and their potential applications in plants and mammalian cells.Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR-associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas-derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have been introduced for engineering the genomes of diverse organisms. The recently introduced prime editing system offers precise editing without many off-target effects than traditional CRISPR-based systems. Many researchers have successfully applied this gene-editing toolbox in diverse systems for various genome-editing applications. This review presents the mechanism of prime editing and summarizes the details of the prime editing system applied in plants and mammalian cells for precise genome editing. We also discuss the advantages, limitations, and potential future applications of prime editing in these systems. This review enables the researcher to gain knowledge on prime editing tools and their potential applications in plants and mammalian cells.
Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR‐associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas‐derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have been introduced for engineering the genomes of diverse organisms. The recently introduced prime editing system offers precise editing without many off‐target effects than traditional CRISPR‐based systems. Many researchers have successfully applied this gene‐editing toolbox in diverse systems for various genome‐editing applications. This review presents the mechanism of prime editing and summarizes the details of the prime editing system applied in plants and mammalian cells for precise genome editing. We also discuss the advantages, limitations, and potential future applications of prime editing in these systems. This review enables the researcher to gain knowledge on prime editing tools and their potential applications in plants and mammalian cells.
Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR‐associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas‐derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have been introduced for engineering the genomes of diverse organisms. The recently introduced prime editing system offers precise editing without many off‐target effects than traditional CRISPR‐based systems. Many researchers have successfully applied this gene‐editing toolbox in diverse systems for various genome‐editing applications. This review presents the mechanism of prime editing and summarizes the details of the prime editing system applied in plants and mammalian cells for precise genome editing. We also discuss the advantages, limitations, and potential future applications of prime editing in these systems. This review enables the researcher to gain knowledge on prime editing tools and their potential applications in plants and mammalian cells.
Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR‐associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas‐derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have been introduced for engineering the genomes of diverse organisms. The recently introduced prime editing system offers precise editing without many off‐target effects than traditional CRISPR‐based systems. Many researchers have successfully applied this gene‐editing toolbox in diverse systems for various genome‐editing applications. This review presents the mechanism of prime editing and summarizes the details of the prime editing system applied in plants and mammalian cells for precise genome editing. We also discuss the advantages, limitations, and potential future applications of prime editing in these systems. This review enables the researcher to gain knowledge on prime editing tools and their potential applications in plants and mammalian cells. Prime editing is a search‐and‐replace genome editing system, which mediates all possible 12 base‐to‐base conversions without DSBs and donor templates at a targeted sequence. We describe how this prime editing system helps in improving genetic studies in plants and mammalian cells with their mechanism, achievements, limitations, and future prospects.
Author Hillary, V. Edwin
Ceasar, S. Antony
Author_xml – sequence: 1
  givenname: V. Edwin
  surname: Hillary
  fullname: Hillary, V. Edwin
  organization: Rajagiri College of Social Sciences
– sequence: 2
  givenname: S. Antony
  orcidid: 0000-0003-4106-1531
  surname: Ceasar
  fullname: Ceasar, S. Antony
  email: antony_sm2003@yahoo.co.in
  organization: Rajagiri College of Social Sciences
BookMark eNqFkU1LxDAQhoMouH5cPQe8eLBrMmmT1psu6wcoCuq5pOl0N9Kma9Mq--9NWVEQxFMYeJ55MzN7ZNu1Dgk54mzKGYOzwqKfAgNgjAnYIhOeAI94qtJtMmEgkyiDWO2SPe9fA5JJiCdk8djZBimWtrduQa2jq1q73lPtStroptG11Y4arGt_Tu_RLLWzvjml2iwtvmODAT6ltW1sr3vbulCMajX0Q4d01bV-hab3B2Sn0rXHw693n7xczZ9nN9Hdw_Xt7OIuMiIVEMkCBa9QFlJXvESFqYpVFhcKWVIZpXlRSUwSEUtTsVICB1RJLBKlspIbnYp9crLpG5LfBvR93lg__l47bAefg-KpEMAk_x-VKWexENmIHv9CX9uhc2GQ0JAlkAJkY_Z0Q5kwte-wyldhubpb55zl44ny8UT594mCEP8SzNcW-07b-m8t22gftsb1PyH55e386cf9BA4xp_E
CitedBy_id crossref_primary_10_1016_j_omtn_2023_07_007
crossref_primary_10_3389_fgene_2023_1204585
crossref_primary_10_1016_j_tibtech_2023_10_007
crossref_primary_10_1016_j_omtn_2025_102635
crossref_primary_10_1007_s12033_022_00567_0
crossref_primary_10_3390_agriculture13050944
crossref_primary_10_1002_cbin_11972
crossref_primary_10_1016_j_biotechadv_2025_108686
crossref_primary_10_1002_1873_3468_70097
crossref_primary_10_1007_s10340_023_01736_z
crossref_primary_10_3389_fgene_2024_1306469
crossref_primary_10_3390_plants12091911
crossref_primary_10_1007_s00299_024_03346_0
crossref_primary_10_1007_s10142_024_01435_7
crossref_primary_10_3389_fgeed_2025_1542487
crossref_primary_10_1007_s42729_022_01025_1
crossref_primary_10_1016_j_gene_2024_149075
crossref_primary_10_1016_j_hlife_2024_06_002
crossref_primary_10_1016_j_stress_2024_100578
crossref_primary_10_1007_s00425_022_04023_w
crossref_primary_10_1007_s11033_023_08521_2
crossref_primary_10_1016_j_omtn_2024_102370
crossref_primary_10_1134_S0026893324010084
Cites_doi 10.1038/nrg2842
10.1016/j.pbi.2016.01.007
10.1016/j.molp.2020.03.011
10.1038/nbt.3803
10.1038/s41586‐019‐1711‐4
10.1016/j.cell.2019.11.030
10.1016/j.ymthe.2021.07.011
10.1016/j.ophtha.2017.04.008
10.1017/S0033583505004063
10.1111/pbi.13395
10.1038/nrm3486
10.1080/15427528.2019.1709596
10.1073/pnas.0900992106
10.2174/1874070701913010173
10.1016/j.ibmb.2016.06.008
10.1038/s41467‐020‐19136‐7
10.1073/pnas.1420294112
10.1038/s41434‐021‐00263‐9
10.1016/j.xplc.2020.100043
10.1016/j.bbamcr.2016.06.009
10.1016/j.chom.2017.07.004
10.1038/nbt.4194
10.1016/j.ibmb.2016.06.004
10.1126/science.aaf8729
10.1001/jamaophthalmol.2020.6418
10.1038/s41467-021-27317-1
10.1098/rstb.2018.0322
10.1016/j.stem.2010.05.016
10.1126/science.1225829
10.1016/j.semcdb.2019.04.008
10.1186/s13059-021-02304-3
10.1089/oli.2006.0065
10.1038/s41587-020-0455-x
10.1038/ncomms15790
10.1007/s00216-018-0873-5
10.1016/j.molmed.2012.09.008
10.1093/jxb/erz415
10.1007/s13205-017-0870-y
10.1016/j.ymben.2018.02.016
10.1093/nar/gku155
10.1016/j.pbi.2018.03.007
10.1111/febs.15079
10.1016/j.jconrel.2014.12.036
10.1038/nmeth.1733
10.1016/j.molp.2016.11.013
10.3389/fphys.2017.00608
10.3390/microorganisms7080269
10.1111/1462-2920.14959
10.3390/ijms20092248
10.1038/nbt.3811
10.34133/2020/9350905
10.1126/science.2660260
10.1038/s41576-018-0059-1
10.1073/pnas.1804428115
10.1038/s41467-018-07226-6
10.1038/s41580-020-00288-9
10.1038/nbt.3547
10.1126/sciadv.aao4774
10.1371/journal.pbio.0030156
10.1016/j.molp.2020.03.010
10.1093/treephys/tps032
10.1111/nph.15777
10.1007/978-3-030-20728-1_5
10.1038/nbt.4148
10.1016/j.tig.2021.10.004
10.3389/fgene.2020.00528
10.1007/978-3-030-75875-2_8
10.1038/nature17946
ContentType Journal Article
Copyright 2022 Wiley Periodicals LLC.
Copyright_xml – notice: 2022 Wiley Periodicals LLC.
DBID AAYXX
CITATION
7QL
7QO
7QP
7QR
7SS
7T7
7TK
7TM
7U9
8FD
C1K
FR3
H94
M7N
P64
RC3
7X8
7S9
L.6
DOI 10.1002/bies.202200032
DatabaseName CrossRef
Bacteriology Abstracts (Microbiology B)
Biotechnology Research Abstracts
Calcium & Calcified Tissue Abstracts
Chemoreception Abstracts
Entomology Abstracts (Full archive)
Industrial and Applied Microbiology Abstracts (Microbiology A)
Neurosciences Abstracts
Nucleic Acids Abstracts
Virology and AIDS Abstracts
Technology Research Database
Environmental Sciences and Pollution Management
Engineering Research Database
AIDS and Cancer Research Abstracts
Algology Mycology and Protozoology Abstracts (Microbiology C)
Biotechnology and BioEngineering Abstracts
Genetics Abstracts
MEDLINE - Academic
AGRICOLA
AGRICOLA - Academic
DatabaseTitle CrossRef
Virology and AIDS Abstracts
Technology Research Database
Nucleic Acids Abstracts
Neurosciences Abstracts
Biotechnology and BioEngineering Abstracts
Environmental Sciences and Pollution Management
Entomology Abstracts
Genetics Abstracts
Biotechnology Research Abstracts
Bacteriology Abstracts (Microbiology B)
Algology Mycology and Protozoology Abstracts (Microbiology C)
AIDS and Cancer Research Abstracts
Chemoreception Abstracts
Engineering Research Database
Industrial and Applied Microbiology Abstracts (Microbiology A)
Calcium & Calcified Tissue Abstracts
MEDLINE - Academic
AGRICOLA
AGRICOLA - Academic
DatabaseTitleList MEDLINE - Academic
AGRICOLA
CrossRef
Virology and AIDS Abstracts
Database_xml – sequence: 1
  dbid: 7X8
  name: MEDLINE - Academic
  url: https://search.proquest.com/medline
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 1521-1878
EndPage n/a
ExternalDocumentID 10_1002_bies_202200032
BIES202200032
Genre article
GroupedDBID ---
-~X
.3N
.GA
.Y3
05W
0R~
10A
1L6
1OB
1OC
1ZS
23N
31~
33P
3SF
3WU
4.4
4ZD
50Y
50Z
51W
51X
52M
52N
52O
52P
52S
52T
52U
52W
52X
53G
5GY
5VS
66C
702
7PT
8-0
8-1
8-3
8-4
8-5
85S
8UM
930
A03
AAESR
AAEVG
AAHBH
AAHHS
AAHQN
AAMNL
AANHP
AANLZ
AAONW
AASGY
AAXRX
AAYCA
AAZKR
ABCQN
ABCUV
ABEFU
ABEML
ABIJN
ABJNI
ABLJU
ABPVW
ABTAH
ACAHQ
ACBWZ
ACCFJ
ACCZN
ACFBH
ACGFS
ACIWK
ACKIV
ACKOT
ACPOU
ACPRK
ACRPL
ACSCC
ACXBN
ACXQS
ACYXJ
ADBBV
ADEOM
ADIZJ
ADKYN
ADMGS
ADNMO
ADOZA
ADXAS
ADZMN
ADZOD
AEEZP
AEIGN
AEIMD
AENEX
AEQDE
AEUQT
AEUYR
AFBPY
AFFPM
AFGKR
AFPWT
AFRAH
AFWVQ
AFZJQ
AHBTC
AITYG
AIURR
AIWBW
AJBDE
AJXKR
ALAGY
ALMA_UNASSIGNED_HOLDINGS
ALUQN
ALVPJ
AMBMR
AMYDB
ASPBG
ATUGU
AUFTA
AVWKF
AZBYB
AZFZN
AZVAB
BAFTC
BDRZF
BFHJK
BHBCM
BMNLL
BMXJE
BNHUX
BQCPF
BROTX
BRXPI
BY8
C45
CS3
D-E
D-F
D0L
DCZOG
DPXWK
DR1
DR2
DRFUL
DRSTM
DU5
EBD
EBS
EJD
EMOBN
F00
F01
F04
F5P
FEDTE
G-S
G.N
GNP
GODZA
H.T
H.X
HBH
HF~
HGLYW
HHY
HHZ
HVGLF
HZ~
IX1
J0M
JPC
KD1
KQQ
LATKE
LAW
LC2
LC3
LEEKS
LH4
LITHE
LOXES
LP6
LP7
LUTES
LW6
LYRES
M56
MEWTI
MK4
MRFUL
MRSTM
MSFUL
MSSTM
MVM
MXFUL
MXSTM
N04
N05
N9A
NF~
NNB
O66
O9-
OIG
OVD
P2P
P2W
P2X
P4D
PALCI
PQQKQ
Q.N
Q11
QB0
QRW
R.K
RIWAO
RJQFR
ROL
RWI
RWR
RX1
RYL
SAMSI
SUPJJ
SV3
TEORI
UB1
UDS
V2E
W8V
W99
WBKPD
WH7
WIB
WIH
WIK
WJL
WNSPC
WOHZO
WQJ
WRC
WXSBR
WYB
WYISQ
XG1
XV2
Y6R
YYQ
YZZ
ZGI
ZUP
ZXP
ZY4
ZZTAW
~IA
~KM
~WT
AAMMB
AAYXX
AEFGJ
AETEA
AEYWJ
AGHNM
AGQPQ
AGXDD
AGYGG
AIDQK
AIDYY
AIQQE
CITATION
O8X
7QL
7QO
7QP
7QR
7SS
7T7
7TK
7TM
7U9
8FD
C1K
FR3
H94
M7N
P64
RC3
7X8
7S9
L.6
ID FETCH-LOGICAL-c3832-6be31fe6b6af1de7e874794b7e05fc7a1bf6e55346cf0d6212e75435779d1ca83
IEDL.DBID DRFUL
ISICitedReferencesCount 25
ISICitedReferencesURI http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000815349300001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN 0265-9247
1521-1878
IngestDate Fri Jul 11 18:28:34 EDT 2025
Thu Oct 02 07:13:46 EDT 2025
Mon Nov 10 02:56:18 EST 2025
Sat Nov 29 05:24:48 EST 2025
Tue Nov 18 21:23:54 EST 2025
Wed Jan 22 16:32:38 EST 2025
IsPeerReviewed true
IsScholarly true
Issue 9
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c3832-6be31fe6b6af1de7e874794b7e05fc7a1bf6e55346cf0d6212e75435779d1ca83
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
ObjectType-Review-3
content type line 23
ORCID 0000-0003-4106-1531
PQID 2705282298
PQPubID 37030
PageCount 13
ParticipantIDs proquest_miscellaneous_2718332061
proquest_miscellaneous_2681043391
proquest_journals_2705282298
crossref_primary_10_1002_bies_202200032
crossref_citationtrail_10_1002_bies_202200032
wiley_primary_10_1002_bies_202200032_BIES202200032
PublicationCentury 2000
PublicationDate September 2022
2022-09-00
20220901
PublicationDateYYYYMMDD 2022-09-01
PublicationDate_xml – month: 09
  year: 2022
  text: September 2022
PublicationDecade 2020
PublicationPlace Cambridge
PublicationPlace_xml – name: Cambridge
PublicationTitle BioEssays
PublicationYear 2022
Publisher Wiley Subscription Services, Inc
Publisher_xml – name: Wiley Subscription Services, Inc
References 2010; 11
2017; 7
2017; 8
2017; 3
2021; 22
2019; 96
2019; 13
2021; 28
2016; 76
2016; 75
2016; 30
2020; 13
2012; 18
2020; 11
2018; 45
2021; 30
2018; 47
2016; 34
2019; 286
2020; 18
2021; 38
2018; 9
2013; 14
2020; 1
2019; 20
2018; 410
2017; 35
2016; 353
2005; 38
2017; 124
2012; 337
2010; 7
2018; 36
2007; 17
2019; 7
2019; 70
2017; 22
2020; 38
2016; 1863
2019; 223
2020; 34
2015; 205
2021; 1
2012; 32
2011; 8
2014; 42
2018; 19
2020; 2020
2021; 12
1989; 244
2021
2020
2021; 139
2017; 10
2015; 112
2018; 115
2019
2019; 179
2018
2019; 576
2005; 3
2016; 533
2020; 22
2020; 21
2016; 28
2018; 14
2009; 106
2019; 374
e_1_2_17_5_1
e_1_2_17_74_1
e_1_2_17_3_1
e_1_2_17_30_1
e_1_2_17_51_1
e_1_2_17_72_1
e_1_2_17_9_1
e_1_2_17_32_1
e_1_2_17_53_1
e_1_2_17_7_1
e_1_2_17_11_1
e_1_2_17_34_1
e_1_2_17_13_1
e_1_2_17_36_1
e_1_2_17_59_1
e_1_2_17_15_1
e_1_2_17_38_1
e_1_2_17_57_1
e_1_2_17_17_1
e_1_2_17_19_1
Lema M. (e_1_2_17_23_1) 2018; 14
e_1_2_17_70_1
e_1_2_17_62_1
e_1_2_17_41_1
e_1_2_17_60_1
e_1_2_17_20_1
e_1_2_17_43_1
e_1_2_17_66_1
e_1_2_17_22_1
e_1_2_17_45_1
e_1_2_17_64_1
e_1_2_17_24_1
e_1_2_17_47_1
e_1_2_17_26_1
e_1_2_17_49_1
e_1_2_17_68_1
e_1_2_17_28_1
Ochoa‐Sanchez A. (e_1_2_17_21_1) 2021
Rousseau J. (e_1_2_17_61_1) 2020
e_1_2_17_50_1
e_1_2_17_73_1
e_1_2_17_4_1
e_1_2_17_52_1
e_1_2_17_71_1
e_1_2_17_2_1
e_1_2_17_54_1
e_1_2_17_8_1
e_1_2_17_10_1
e_1_2_17_56_1
e_1_2_17_75_1
e_1_2_17_6_1
e_1_2_17_12_1
e_1_2_17_33_1
e_1_2_17_14_1
e_1_2_17_35_1
e_1_2_17_16_1
e_1_2_17_37_1
e_1_2_17_58_1
e_1_2_17_18_1
e_1_2_17_39_1
Altpeter F. (e_1_2_17_31_1) 2016; 28
e_1_2_17_63_1
e_1_2_17_40_1
e_1_2_17_42_1
e_1_2_17_67_1
e_1_2_17_44_1
e_1_2_17_65_1
e_1_2_17_25_1
e_1_2_17_46_1
Wu K. (e_1_2_17_55_1) 2018
e_1_2_17_27_1
e_1_2_17_48_1
e_1_2_17_69_1
e_1_2_17_29_1
References_xml – volume: 223
  start-page: 293
  issue: 1
  year: 2019
  end-page: 309
  article-title: Multitrait genome‐wide association analysis of Populus trichocarpa identifies key polymorphisms controlling morphological and physiological traits
  publication-title: New Phytologist
– volume: 14
  year: 2018
  article-title: Marker assisted selection in comparison to conventional plant breeding
  publication-title: Agric. Res. Technol
– volume: 75
  start-page: 98
  year: 2016
  end-page: 106
  article-title: CRISPR/Cas9 mediated knockout of the abdominal‐A homeotic gene in the global pest, diamondback moth (Plutella xylostella)
  publication-title: Insect Biochemistry and Molecular Biology
– volume: 20
  start-page: 2248
  issue: 9
  year: 2019
  article-title: Knock‐down of gossypol‐inducing cytochrome P450 genes reduced deltamethrin sensitivity in Spodoptera exigua (Hübner)
  publication-title: International Journal of Molecular Sciences
– volume: 18
  start-page: 679
  issue: 11
  year: 2012
  end-page: 688
  article-title: Sense from nonsense: Therapies for premature stop codon diseases
  publication-title: Trends in Molecular Medicine
– volume: 19
  start-page: 770
  issue: 12
  year: 2018
  end-page: 788
  article-title: Base editing: Precision chemistry on the genome and transcriptome of living cells
  publication-title: Nature Reviews Genetics
– volume: 35
  start-page: 438
  issue: 5
  year: 2017
  end-page: 440
  article-title: Precise base editing in rice, wheat and maize with a Cas9‐cytidine deaminase fusion
  publication-title: Nature Biotechnology
– volume: 3
  issue: 8
  year: 2017
  article-title: Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C: G‐to‐T: A base editors with higher efficiency and product purity
  publication-title: Science Advances
– volume: 28
  start-page: 396
  issue: 7
  year: 2021
  end-page: 401
  article-title: Prime editing – an update on the field
  publication-title: Gene Therapy
– start-page: 291
  year: 2021
  end-page: 317
– volume: 1
  issue: 3
  year: 2020
  article-title: Development of plant prime‐editing systems for precise genome editing
  publication-title: Plant Communications
– volume: 115
  start-page: 11573
  issue: 45
  year: 2018
  end-page: 11578
  article-title: Association mapping, transcriptomics, and transient expression identify candidate genes mediating plant–pathogen interactions in a tree
  publication-title: Proceedings of the National Academy of Sciences
– volume: 35
  start-page: 371
  issue: 4
  year: 2017
  end-page: 376
  article-title: Increasing the genome‐targeting scope and precision of base editing with engineered Cas9‐cytidine deaminase fusions
  publication-title: Nature Biotechnology
– volume: 8
  start-page: 608
  year: 2017
  article-title: Progress and prospects of CRISPR/Cas systems in insects and other arthropods
  publication-title: Frontiers in Physiology
– volume: 21
  start-page: 661
  issue: 11
  year: 2020
  end-page: 677
  article-title: Applications of CRISPR–Cas in agriculture and plant biotechnology
  publication-title: Nature Reviews Molecular Cell Biology
– volume: 38
  start-page: 218
  issue: 3
  year: 2021
  end-page: 222
  article-title: Potato trait development going fast‐forward with genome editing
  publication-title: Trends in Genetics
– volume: 96
  start-page: 65
  year: 2019
  end-page: 76
  article-title: Improving plant‐resistance to insect‐pests and pathogens: The new opportunities through targeted genome editing
  publication-title: Seminars in Cell & Developmental Biology
– volume: 42
  start-page: 5390
  issue: 8
  year: 2014
  end-page: 5402
  article-title: Comprehensive analysis of the specificity of transcription activator‐like effector nucleases
  publication-title: Nucleic Acids Research
– volume: 11
  start-page: 1
  issue: 528
  year: 2020
  end-page: 6
  article-title: Prime editing: Genome editing for rare genetic diseases without double‐strand breaks or donor DNA
  publication-title: Frontiers in Genetics
– volume: 14
  start-page: 49
  issue: 1
  year: 2013
  end-page: 55
  article-title: TALENs: A widely applicable technology for targeted genome editing
  publication-title: Nature Reviews Molecular Cell Biology
– start-page: 13
  year: 2018
  article-title: CRISPR/Cas9 mediated knockout of the abdominal‐A homeotic gene in fall armyworm moth (Spodoptera frugiperda)
  publication-title: PLoS ONE
– volume: 179
  start-page: 1448
  issue: 7
  year: 2019
  end-page: 1450
  article-title: One prime for all editing
  publication-title: Cell
– volume: 374
  issue: 1767
  year: 2019
  article-title: Genome editing for plant disease resistance: Applications and perspectives
  publication-title: Philosophical Transactions of the Royal Society B
– volume: 1
  start-page: 75
  issue: 1
  year: 2021
  end-page: 82
– volume: 30
  start-page: 70
  year: 2016
  end-page: 77
  article-title: Advances and perspectives on the use of CRISPR/Cas9 systems in plant genomics research
  publication-title: Current Opinion in Plant Biology
– volume: 9
  start-page: 4820
  issue: 1
  year: 2018
  article-title: CYP6AE gene cluster knockout in Helicoverpa armigera reveals role in detoxification of phytochemicals and insecticides
  publication-title: Nature Communications
– volume: 7
  start-page: 269
  issue: 8
  year: 2019
  article-title: Exploration of plant‐microbe interactions for sustainable agriculture in CRISPR era
  publication-title: Microorganisms
– volume: 13
  start-page: 671
  issue: 5
  year: 2020
  end-page: 674
  article-title: Precise modifications of both exogenous and endogenous genes in rice by prime editing
  publication-title: Molecular Plant
– volume: 1863
  start-page: 2333
  issue: 9
  year: 2016
  end-page: 2344
– volume: 47
  start-page: 200
  year: 2018
  end-page: 210
  article-title: MACBETH: Multiplex automated Corynebacterium glutamicum base editing method
  publication-title: Metabolic Engineering
– volume: 12
  start-page: 1
  issue: 1
  year: 2021
  end-page: 13
  article-title: Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice
  publication-title: Nature Communications
– volume: 139
  start-page: 319
  issue: 3
  year: 2021
  end-page: 328
  article-title: Analysis of pathogenic variants correctable with CRISPR base editing among patients with recessive inherited retinal degeneration
  publication-title: JAMA Ophthalmology
– volume: 7
  start-page: 186
  issue: 2
  year: 2010
  end-page: 197
  article-title: A distinctive DNA damage response in human hematopoietic stem cells reveals an apoptosis‐independent role for p53 in self‐renewal
  publication-title: Cell Stem Cell
– volume: 8
  start-page: 941
  issue: 11
  year: 2011
  end-page: 943
  article-title: Surrogate reporters for enrichment of cells with nuclease‐induced mutations
  publication-title: Nature Methods
– volume: 244
  start-page: 1288
  issue: 4910
  year: 1989
  end-page: 1292
  article-title: Altering the genome by homologous recombination
  publication-title: Science
– volume: 28
  start-page: 1510
  issue: 7
  year: 2016
  end-page: 1520
  article-title: Advancing crop transformation in the era of genome editing
  publication-title: The Plant Cell
– volume: 337
  start-page: 816
  issue: 6096
  year: 2012
  end-page: 821
  article-title: A programmable dual‐RNA–guided DNA endonuclease in adaptive bacterial immunity
  publication-title: Science
– volume: 36
  start-page: 888
  issue: 9
  year: 2018
  end-page: 893
  article-title: Optimized base editors enable efficient editing in cells, organoids and mice
  publication-title: Nature Biotechnology
– volume: 32
  start-page: 626
  issue: 5
  year: 2012
  end-page: 638
  article-title: Identification of quantitative trait loci and candidate genes for cadmium tolerance in Populus
  publication-title: Tree Physiology
– volume: 22
  start-page: 1435
  issue: 4
  year: 2020
  end-page: 1446
  article-title: The small secreted effector protein MiSSP7. 6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis
  publication-title: Environmental Microbiology
– volume: 34
  start-page: 335
  issue: 3
  year: 2020
  end-page: 355
  article-title: Hybridization and hybrid detection through molecular markers in finger millet [Eleusine coracana (L.) Gaertn.]
  publication-title: Journal of Crop Improvement
– volume: 7
  start-page: 1
  issue: 4
  year: 2017
  end-page: 35
  article-title: Genetic engineering strategies for biotic and abiotic stress tolerance and quality enhancement in horticultural crops: a comprehensive review
  publication-title: 3 Biotech
– volume: 38
  start-page: 582
  issue: 5
  year: 2020
  end-page: 585
  article-title: Prime genome editing in rice and wheat
  publication-title: Nature Biotechnology
– volume: 13
  start-page: 667
  issue: 5
  year: 2020
  end-page: 670
  article-title: Plant prime editors enable precise gene editing in rice cells
  publication-title: Molecular Plant
– volume: 533
  start-page: 420
  issue: 7603
  year: 2016
  end-page: 424
  article-title: Programmable editing of a target base in genomic DNA without double‐stranded DNA cleavage
  publication-title: Nature
– volume: 576
  start-page: 149
  issue: 7785
  year: 2019
  end-page: 157
  article-title: Search‐and‐replace genome editing without double‐strand breaks or donor DNA
  publication-title: Nature
– volume: 76
  start-page: 11
  year: 2016
  end-page: 17
  article-title: Functional validation of cadherin as a receptor of Bt toxin Cry1Ac in Helicoverpa armigera utilizing the CRISPR/Cas9 system
  publication-title: Insect Biochemistry and Molecular Biology
– start-page: 1
  year: 2020
  end-page: 11
  article-title: Specific mutations in genes responsible for Alzheimer and for Duchenne muscular dystrophy introduced by base editing and PRIME editing
  publication-title: BioRxiv
– volume: 106
  start-page: 12273
  issue: 30
  year: 2009
  end-page: 12278
  article-title: Genomewide SNP variation reveals relationships among landraces and modern varieties of rice
  publication-title: Proceedings of the National Academy of Sciences
– volume: 36
  start-page: 536
  issue: 6
  year: 2018
  end-page: 539
  article-title: Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy
  publication-title: Nature Biotechnology
– volume: 45
  start-page: 205
  year: 2018
  end-page: 211
  article-title: Can genomics deliver climate‐change ready crops?
  publication-title: Current Opinion in Plant Biology
– volume: 70
  start-page: 6621
  issue: 22
  year: 2019
  end-page: 6629
  article-title: CRISPR/Cas9‐mediated targeted mutagenesis for functional genomics research of crassulacean acid metabolism plants
  publication-title: Journal of Experimental Botany
– volume: 22
  start-page: 142
  issue: 2
  year: 2017
  end-page: 155
  article-title: Establishing causality: Opportunities of synthetic communities for plant microbiome research
  publication-title: Cell Host & Microbe
– start-page: 83
  year: 2019
  end-page: 111
– volume: 205
  start-page: 120
  year: 2015
  end-page: 127
  article-title: Current and future delivery systems for engineered nucleases: ZFN, TALEN and RGEN
  publication-title: Journal of Controlled Release
– volume: 18
  start-page: 2167
  issue: 11
  year: 2020
  article-title: Precision genome engineering in rice using prime editing system
  publication-title: Plant Biotechnology Journal
– volume: 410
  start-page: 2889
  issue: 12
  year: 2018
  end-page: 2900
  article-title: Detection of target DNA with a novel Cas9/sgRNAs‐associated reverse PCR (CARP) technique
  publication-title: Analytical and Bioanalytical Chemistry
– volume: 30
  start-page: 283
  issue: 1
  year: 2021
  end-page: 294
  article-title: Dual‐AAV delivering split prime editor system for in vivo genome editing
  publication-title: Molecular Therapy
– volume: 13
  start-page: 173
  issue: 1
  year: 2019
  end-page: 179
  article-title: Application of CRISPR/Cas9 genome editing system in cereal crops
  publication-title: The Open Biotechnology Journal
– volume: 112
  start-page: 3570
  issue: 11
  year: 2015
  end-page: 3575
  article-title: Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA‐processing system
  publication-title: Proceedings of the National Academy of Sciences
– volume: 286
  start-page: 4657
  issue: 23
  year: 2019
  end-page: 4660
  article-title: More precise, more universal and more specific–the next generation of RNA‐guided endonucleases for genome editing
  publication-title: The FEBS Journal
– volume: 10
  start-page: 523
  issue: 3
  year: 2017
  end-page: 525
  article-title: Precise editing of a target base in the rice genome using a modified CRISPR/Cas9 system
  publication-title: Molecular Plant
– volume: 34
  start-page: 768
  issue: 7
  year: 2016
  end-page: 73
  article-title: DNA‐guided genome editing using the Natronobacterium gregoryi Argonaute
  publication-title: Nature Biotechnology
– volume: 38
  start-page: 49
  issue: 1
  year: 2005
  end-page: 95
  article-title: Homing endonuclease structure and function
  publication-title: Quarterly Reviews of Biophysics
– volume: 22
  start-page: 1
  issue: 1
  year: 2021
  end-page: 21
  article-title: Prime editing in mice reveals the essentiality of a single base in driving tissue‐specific gene expression
  publication-title: Genome Biology
– volume: 124
  start-page: 1314
  issue: 9
  year: 2017
  end-page: 1331
  article-title: Clinically focused molecular investigation of 1000 consecutive families with inherited retinal disease
  publication-title: Ophthalmology
– volume: 11
  start-page: 636
  issue: 9
  year: 2010
  end-page: 646
  article-title: Genome editing with engineered zinc finger nucleases
  publication-title: Nature Reviews Genetics
– volume: 2020
  year: 2020
  article-title: Prime editing technology and its prospects for future applications in plant biology research
  publication-title: BioDesign Research
– volume: 11
  start-page: 1
  issue: 1
  year: 2020
  end-page: 8
  article-title: Prime editing for functional repair in patient‐derived disease models
  publication-title: Nature Communications
– volume: 17
  start-page: 237
  issue: 2
  year: 2007
  end-page: 250
  article-title: Insights into effective RNAi gained from large‐scale siRNA validation screening
  publication-title: Oligonucleotides
– volume: 8
  start-page: 1
  issue: 1
  year: 2017
  end-page: 10
  article-title: Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery
  publication-title: Nature Communications
– volume: 3
  issue: 5
  year: 2005
  article-title: Subversion of cellular autophagosomal machinery by RNA viruses
  publication-title: PLoS Biology
– volume: 353
  issue: 6305
  year: 2016
  article-title: Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems
  publication-title: Science
– ident: e_1_2_17_6_1
  doi: 10.1038/nrg2842
– ident: e_1_2_17_40_1
  doi: 10.1016/j.pbi.2016.01.007
– ident: e_1_2_17_68_1
  doi: 10.1016/j.molp.2020.03.011
– ident: e_1_2_17_15_1
  doi: 10.1038/nbt.3803
– ident: e_1_2_17_17_1
  doi: 10.1038/s41586‐019‐1711‐4
– ident: e_1_2_17_38_1
  doi: 10.1016/j.cell.2019.11.030
– ident: e_1_2_17_58_1
  doi: 10.1016/j.ymthe.2021.07.011
– ident: e_1_2_17_65_1
  doi: 10.1016/j.ophtha.2017.04.008
– ident: e_1_2_17_5_1
  doi: 10.1017/S0033583505004063
– ident: e_1_2_17_75_1
  doi: 10.1111/pbi.13395
– ident: e_1_2_17_7_1
  doi: 10.1038/nrm3486
– start-page: 75
  volume-title: Re: GEN Open
  year: 2021
  ident: e_1_2_17_21_1
– ident: e_1_2_17_22_1
  doi: 10.1080/15427528.2019.1709596
– ident: e_1_2_17_46_1
  doi: 10.1073/pnas.0900992106
– ident: e_1_2_17_29_1
  doi: 10.2174/1874070701913010173
– ident: e_1_2_17_52_1
  doi: 10.1016/j.ibmb.2016.06.008
– ident: e_1_2_17_60_1
  doi: 10.1038/s41467‐020‐19136‐7
– ident: e_1_2_17_69_1
  doi: 10.1073/pnas.1420294112
– ident: e_1_2_17_18_1
  doi: 10.1038/s41434‐021‐00263‐9
– ident: e_1_2_17_74_1
  doi: 10.1016/j.xplc.2020.100043
– ident: e_1_2_17_28_1
  doi: 10.1016/j.bbamcr.2016.06.009
– ident: e_1_2_17_49_1
  doi: 10.1016/j.chom.2017.07.004
– ident: e_1_2_17_70_1
  doi: 10.1038/nbt.4194
– ident: e_1_2_17_56_1
  doi: 10.1016/j.ibmb.2016.06.004
– ident: e_1_2_17_12_1
  doi: 10.1126/science.aaf8729
– ident: e_1_2_17_64_1
  doi: 10.1001/jamaophthalmol.2020.6418
– ident: e_1_2_17_59_1
  doi: 10.1038/s41467-021-27317-1
– ident: e_1_2_17_47_1
  doi: 10.1098/rstb.2018.0322
– ident: e_1_2_17_63_1
  doi: 10.1016/j.stem.2010.05.016
– ident: e_1_2_17_9_1
  doi: 10.1126/science.1225829
– ident: e_1_2_17_50_1
  doi: 10.1016/j.semcdb.2019.04.008
– ident: e_1_2_17_62_1
  doi: 10.1186/s13059-021-02304-3
– ident: e_1_2_17_4_1
  doi: 10.1089/oli.2006.0065
– ident: e_1_2_17_35_1
  doi: 10.1038/s41587-020-0455-x
– ident: e_1_2_17_16_1
  doi: 10.1038/ncomms15790
– ident: e_1_2_17_43_1
  doi: 10.1007/s00216-018-0873-5
– ident: e_1_2_17_66_1
  doi: 10.1016/j.molmed.2012.09.008
– ident: e_1_2_17_39_1
  doi: 10.1093/jxb/erz415
– ident: e_1_2_17_26_1
  doi: 10.1007/s13205-017-0870-y
– ident: e_1_2_17_41_1
  doi: 10.1016/j.ymben.2018.02.016
– ident: e_1_2_17_8_1
  doi: 10.1093/nar/gku155
– ident: e_1_2_17_27_1
  doi: 10.1016/j.pbi.2018.03.007
– ident: e_1_2_17_10_1
  doi: 10.1111/febs.15079
– start-page: 13
  year: 2018
  ident: e_1_2_17_55_1
  article-title: CRISPR/Cas9 mediated knockout of the abdominal‐A homeotic gene in fall armyworm moth (Spodoptera frugiperda)
  publication-title: PLoS ONE
– ident: e_1_2_17_57_1
– ident: e_1_2_17_71_1
  doi: 10.1016/j.jconrel.2014.12.036
– ident: e_1_2_17_73_1
  doi: 10.1038/nmeth.1733
– ident: e_1_2_17_32_1
  doi: 10.1016/j.molp.2016.11.013
– volume: 28
  start-page: 1510
  issue: 7
  year: 2016
  ident: e_1_2_17_31_1
  article-title: Advancing crop transformation in the era of genome editing
  publication-title: The Plant Cell
– ident: e_1_2_17_51_1
  doi: 10.3389/fphys.2017.00608
– ident: e_1_2_17_48_1
  doi: 10.3390/microorganisms7080269
– ident: e_1_2_17_34_1
  doi: 10.1111/1462-2920.14959
– ident: e_1_2_17_53_1
  doi: 10.3390/ijms20092248
– ident: e_1_2_17_33_1
  doi: 10.1038/nbt.3811
– ident: e_1_2_17_20_1
  doi: 10.34133/2020/9350905
– start-page: 1
  year: 2020
  ident: e_1_2_17_61_1
  article-title: Specific mutations in genes responsible for Alzheimer and for Duchenne muscular dystrophy introduced by base editing and PRIME editing
  publication-title: BioRxiv
– ident: e_1_2_17_2_1
  doi: 10.1126/science.2660260
– ident: e_1_2_17_14_1
  doi: 10.1038/s41576-018-0059-1
– ident: e_1_2_17_45_1
  doi: 10.1073/pnas.1804428115
– ident: e_1_2_17_54_1
  doi: 10.1038/s41467-018-07226-6
– ident: e_1_2_17_30_1
  doi: 10.1038/s41580-020-00288-9
– ident: e_1_2_17_72_1
  doi: 10.1038/nbt.3547
– ident: e_1_2_17_13_1
  doi: 10.1126/sciadv.aao4774
– ident: e_1_2_17_3_1
  doi: 10.1371/journal.pbio.0030156
– ident: e_1_2_17_36_1
  doi: 10.1016/j.molp.2020.03.010
– volume: 14
  year: 2018
  ident: e_1_2_17_23_1
  article-title: Marker assisted selection in comparison to conventional plant breeding
  publication-title: Agric. Res. Technol
– ident: e_1_2_17_44_1
  doi: 10.1093/treephys/tps032
– ident: e_1_2_17_42_1
  doi: 10.1111/nph.15777
– ident: e_1_2_17_25_1
  doi: 10.1007/978-3-030-20728-1_5
– ident: e_1_2_17_67_1
  doi: 10.1038/nbt.4148
– ident: e_1_2_17_37_1
  doi: 10.1016/j.tig.2021.10.004
– ident: e_1_2_17_19_1
  doi: 10.3389/fgene.2020.00528
– ident: e_1_2_17_24_1
  doi: 10.1007/978-3-030-75875-2_8
– ident: e_1_2_17_11_1
  doi: 10.1038/nature17946
SSID ssj0009624
Score 2.506722
SecondaryResourceType review_article
Snippet Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR‐associated protein (CRISPR/Cas) system has revolutionized genetic research in the...
Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR-associated protein (CRISPR/Cas) system has revolutionized genetic research in the...
SourceID proquest
crossref
wiley
SourceType Aggregation Database
Enrichment Source
Index Database
Publisher
StartPage e2200032
SubjectTerms base editing
Cas9 H840 nickase
CRISPR
CRISPR/Cas
Editing
gene editing
genome
Genomes
Mammalian cells
Mammals
Nuclease
pegRNA
plants
prime editing
Recombinase
recombinases
researchers
Title Prime editing in plants and mammalian cells: Mechanism, achievements, limitations, and future prospects
URI https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fbies.202200032
https://www.proquest.com/docview/2705282298
https://www.proquest.com/docview/2681043391
https://www.proquest.com/docview/2718332061
Volume 44
WOSCitedRecordID wos000815349300001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVWIB
  databaseName: Wiley Online Library Full Collection 2020
  customDbUrl:
  eissn: 1521-1878
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0009624
  issn: 0265-9247
  databaseCode: DRFUL
  dateStart: 19980101
  isFulltext: true
  titleUrlDefault: https://onlinelibrary.wiley.com
  providerName: Wiley-Blackwell
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwEB7BFiQuvBELpTISEpe1GsfxI9x4rThAVSEq7S1yHButtJtWzbZS_z0zcTbbHgAJbok8jpyxx_PQzDcAb6xGnRB84LoRkhcmKu5EFFx6XQY02JssS80mzNGRXSzK42tV_AkfYgy4kWT09zUJuKu7wx1oaE2eZE6Fongw8RLey_HwFhPY-_R9fvJ1B7yr-8a26Goojr6G2QI3ZvnhzS_cVEw7a_O6zdornfmD_1_uQ7g_GJzsfTohj-BWaB_D3dSC8uoJ_DwmfH-GKozyn9myZWcrSo1hrm3Y2q3XfRyEUXy_e8e-BSoUXnbrGaMkzNCDjW-6GVtRnVQK_s36qQmrhOEP9rWc3VM4mX_-8fELH5ovcI9Oa851HaSIQdfaRdEEE6whMPrahExFb5yoow5KyUL7mDUaNWAwCm0vY8pGeGflM5i0p214DiwXUsVa6RhlVgRprbeZd41AT61GejMFvuV85YfFUoOMVZUwlfOKmFeNzJvC25H-LGFy_JZyf7uR1SCbOGoyRcmzpZ3C63EYpYpY6dpweoE0BNNWSFmKP9CgWpcyR4NoCnm_9X9ZTfUBLfzx7cW_THoJ9-g5Jbntw2RzfhFewR1_uVl25wdw2yzswSABvwD1sgSL
linkProvider Wiley-Blackwell
linkToHtml http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3da9swED9K2rG9bN1Habqu1WCwl4haliXZe9tHQ8fSUEYLfTO2LJVA4pY6HfS_353lOOvDNhh7tHUy8kmn--DudwDvUo06wVnHdSUkT4xXvBBecGl15tBgr6IoNJsw02l6eZmdddmEVAsT8CH6gBtJRntfk4BTQPpojRpakisZU6Uonky8hTcTPEtqAJtfvo8vJmvkXd12tkVfQ3F0NswKuTGKjx5-4aFmWpubvxqtrdYZP_sP692Gp53JyT6GM_IcNlz9Ah6FJpT3L-HqjBD-GSoxyoBms5rdzCk5hhV1xRbFYtFGQhhF-JsP7NRRqfCsWYwYpWG6Fm582YzYnCqlQvhv1E4NaCUM_7Ct5mxewcX4-PzzCe_aL3CLbmvMdemk8E6XuvCicsalhuDoS-Mi5a0pROm1U0om2vqo0qgDnVFofRmTVcIWqdyBQX1du11gsZDKl0p7L6PEyTS1aWSLSqCvViK9GQJfsT633WKpRcY8D6jKcU7My3vmDeF9T38TUDl-S7m_2sm8k04cNZGi9NksHcLbfhjlilhZ1O76DmkIqC2RMhN_oEHFLmWMJtEQ4nbv_7Ka_BPa-P3T3r9MOoTHJ-enk3zydfrtNTyh9yHlbR8Gy9s79wa27I_lrLk96AThJ3SqB5M
linkToPdf http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3da9swEBel3cZe9l2are1UGOwlopZlSfbe1o_QsTaEsULfjC2fSiBxQ50O9t_vznKc9WErjD3aOhn5pNN9cPc7xj6kBnUCOBCmkkok1mtRSC-FciYDNNirKArNJux4nF5dZZMum5BqYQI-RB9wI8lo72sScFhU_nCNGlqSKxlTpSieTLyFtxKdGZTNrZNvo8vzNfKuaTvboq-hBTobdoXcGMWH979wXzOtzc3fjdZW64ye_4f1vmDPOpOTfw5n5CXbgPoVexyaUP58za4nhPDPUYlRBjSf1nwxo-QYXtQVnxfzeRsJ4RThbz7xC6BS4WkzH3JKw4QWbnzZDPmMKqVC-G_YTg1oJRz_sK3mbN6wy9Hp9-Mz0bVfEA7d1liYEpT0YEpTeFmBhdQSHH1pIdLe2UKW3oDWKjHOR5VBHQhWo_VlbVZJV6Rqm23WNzXsMB5LpX2pjfcqSkClqUsjV1QSfbUS6e2AiRXrc9ctllpkzPKAqhznxLy8Z96AfezpFwGV44-Uu6udzDvpxFEbaUqfzdIBO-iHUa6IlUUNN3dIQ0BtiVKZ_AsNKnalYjSJBixu9_6B1eRHaOP3T2__ZdJ79mRyMsrPv4y_vmNP6XXIeNtlm8vbO9hjj9yP5bS53e_k4BfycwcO
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Prime+editing+in+plants+and+mammalian+cells%3A+Mechanism%2C+achievements%2C+limitations%2C+and+future+prospects&rft.jtitle=BioEssays&rft.au=Hillary%2C+V+Edwin&rft.au=Ceasar%2C+S.+Antony&rft.date=2022-09-01&rft.issn=0265-9247&rft.volume=44&rft.issue=9+p.e2200032-&rft_id=info:doi/10.1002%2Fbies.202200032&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0265-9247&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0265-9247&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0265-9247&client=summon