Molecular analysis of jejunal, ileal, caecal and recto-sigmoidal human colonic microbiota using 16S rRNA gene libraries and terminal restriction fragment length polymorphism

Microbiota in gut contents of jejunum, ileum, caecum and recto-sigmoid colon obtained from three elderly individuals at autopsy were compared using 16S rRNA gene libraries and terminal restriction fragment length polymorphism (T-RFLP). Random clones of 16S rRNA gene sequences were isolated after PCR...

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Bibliographic Details
Published in:	Journal of medical microbiology Vol. 54; no. Pt 11; p. 1093
Main Authors:	Hayashi, Hidenori, Takahashi, Rei, Nishi, Takahiro, Sakamoto, Mitsuo, Benno, Yoshimi
Format:	Journal Article
Language:	English
Published:	England 01.11.2005
Subjects:	Aged Bacteria - classification Bacteria - genetics Cecum - microbiology Colon, Sigmoid - microbiology Deoxyribonuclease HpaII - metabolism Deoxyribonucleases, Type II Site-Specific - metabolism DNA Fingerprinting DNA, Bacterial - analysis DNA, Bacterial - chemistry DNA, Bacterial - isolation & purification DNA, Ribosomal - genetics Female Humans Ileum - microbiology Intestines - microbiology Jejunum - microbiology Male Molecular Sequence Data Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA
ISSN:	0022-2615
Online Access:	Get more information
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