Molecular analysis of jejunal, ileal, caecal and recto-sigmoidal human colonic microbiota using 16S rRNA gene libraries and terminal restriction fragment length polymorphism
Microbiota in gut contents of jejunum, ileum, caecum and recto-sigmoid colon obtained from three elderly individuals at autopsy were compared using 16S rRNA gene libraries and terminal restriction fragment length polymorphism (T-RFLP). Random clones of 16S rRNA gene sequences were isolated after PCR...
Uložené v:
| Vydané v: | Journal of medical microbiology Ročník 54; číslo Pt 11; s. 1093 |
|---|---|
| Hlavní autori: | , , , , |
| Médium: | Journal Article |
| Jazyk: | English |
| Vydavateľské údaje: |
England
01.11.2005
|
| Predmet: | |
| ISSN: | 0022-2615 |
| On-line prístup: | Zistit podrobnosti o prístupe |
| Tagy: |
Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
|
| Abstract | Microbiota in gut contents of jejunum, ileum, caecum and recto-sigmoid colon obtained from three elderly individuals at autopsy were compared using 16S rRNA gene libraries and terminal restriction fragment length polymorphism (T-RFLP). Random clones of 16S rRNA gene sequences were isolated after PCR amplification with universal primer sets of total genomic DNA extracted from each sample of gut contents. An average of 90 randomly selected clones were partially sequenced (about 500 bp). T-RFLP analysis was performed using the 16S rRNA gene amplified from each sample. The lengths of the terminal restriction fragments were analysed after digestion with HhaI and MspI. The jejunal and ileal microbiota consisted of simple microbial communities of streptococci, lactobacilli, 'Gammaproteobacteria', the Enterococcus group and the Bacteroides group. Most of the species were facultative anaerobes or aerobes. The Clostridium coccoides group and the Clostridium leptum subgroup, which are the most predominant groups in human faeces, were not detected in samples from the upper gastrointestinal tract. The caecal microbiota was more complex than the jejunal and ileal microbiota. The C. coccoides group, the C. leptum subgroup and the Bacteroides group were detected in the caecum. The recto-sigmoidal colonic microbiota consisted of complex microbial communities, with numerous species that belonged to the C. coccoides group, the C. leptum subgroup, the Bacteroides group, 'Gammaproteobacteria', the Bifidobacterium group, streptococci and lactobacilli, and included more than 26 operational taxonomic units. The results showed marked individual differences in the composition of microbiota in each region. |
|---|---|
| AbstractList | Microbiota in gut contents of jejunum, ileum, caecum and recto-sigmoid colon obtained from three elderly individuals at autopsy were compared using 16S rRNA gene libraries and terminal restriction fragment length polymorphism (T-RFLP). Random clones of 16S rRNA gene sequences were isolated after PCR amplification with universal primer sets of total genomic DNA extracted from each sample of gut contents. An average of 90 randomly selected clones were partially sequenced (about 500 bp). T-RFLP analysis was performed using the 16S rRNA gene amplified from each sample. The lengths of the terminal restriction fragments were analysed after digestion with HhaI and MspI. The jejunal and ileal microbiota consisted of simple microbial communities of streptococci, lactobacilli, 'Gammaproteobacteria', the Enterococcus group and the Bacteroides group. Most of the species were facultative anaerobes or aerobes. The Clostridium coccoides group and the Clostridium leptum subgroup, which are the most predominant groups in human faeces, were not detected in samples from the upper gastrointestinal tract. The caecal microbiota was more complex than the jejunal and ileal microbiota. The C. coccoides group, the C. leptum subgroup and the Bacteroides group were detected in the caecum. The recto-sigmoidal colonic microbiota consisted of complex microbial communities, with numerous species that belonged to the C. coccoides group, the C. leptum subgroup, the Bacteroides group, 'Gammaproteobacteria', the Bifidobacterium group, streptococci and lactobacilli, and included more than 26 operational taxonomic units. The results showed marked individual differences in the composition of microbiota in each region. Microbiota in gut contents of jejunum, ileum, caecum and recto-sigmoid colon obtained from three elderly individuals at autopsy were compared using 16S rRNA gene libraries and terminal restriction fragment length polymorphism (T-RFLP). Random clones of 16S rRNA gene sequences were isolated after PCR amplification with universal primer sets of total genomic DNA extracted from each sample of gut contents. An average of 90 randomly selected clones were partially sequenced (about 500 bp). T-RFLP analysis was performed using the 16S rRNA gene amplified from each sample. The lengths of the terminal restriction fragments were analysed after digestion with HhaI and MspI. The jejunal and ileal microbiota consisted of simple microbial communities of streptococci, lactobacilli, 'Gammaproteobacteria', the Enterococcus group and the Bacteroides group. Most of the species were facultative anaerobes or aerobes. The Clostridium coccoides group and the Clostridium leptum subgroup, which are the most predominant groups in human faeces, were not detected in samples from the upper gastrointestinal tract. The caecal microbiota was more complex than the jejunal and ileal microbiota. The C. coccoides group, the C. leptum subgroup and the Bacteroides group were detected in the caecum. The recto-sigmoidal colonic microbiota consisted of complex microbial communities, with numerous species that belonged to the C. coccoides group, the C. leptum subgroup, the Bacteroides group, 'Gammaproteobacteria', the Bifidobacterium group, streptococci and lactobacilli, and included more than 26 operational taxonomic units. The results showed marked individual differences in the composition of microbiota in each region.Microbiota in gut contents of jejunum, ileum, caecum and recto-sigmoid colon obtained from three elderly individuals at autopsy were compared using 16S rRNA gene libraries and terminal restriction fragment length polymorphism (T-RFLP). Random clones of 16S rRNA gene sequences were isolated after PCR amplification with universal primer sets of total genomic DNA extracted from each sample of gut contents. An average of 90 randomly selected clones were partially sequenced (about 500 bp). T-RFLP analysis was performed using the 16S rRNA gene amplified from each sample. The lengths of the terminal restriction fragments were analysed after digestion with HhaI and MspI. The jejunal and ileal microbiota consisted of simple microbial communities of streptococci, lactobacilli, 'Gammaproteobacteria', the Enterococcus group and the Bacteroides group. Most of the species were facultative anaerobes or aerobes. The Clostridium coccoides group and the Clostridium leptum subgroup, which are the most predominant groups in human faeces, were not detected in samples from the upper gastrointestinal tract. The caecal microbiota was more complex than the jejunal and ileal microbiota. The C. coccoides group, the C. leptum subgroup and the Bacteroides group were detected in the caecum. The recto-sigmoidal colonic microbiota consisted of complex microbial communities, with numerous species that belonged to the C. coccoides group, the C. leptum subgroup, the Bacteroides group, 'Gammaproteobacteria', the Bifidobacterium group, streptococci and lactobacilli, and included more than 26 operational taxonomic units. The results showed marked individual differences in the composition of microbiota in each region. |
| Author | Takahashi, Rei Benno, Yoshimi Hayashi, Hidenori Nishi, Takahiro Sakamoto, Mitsuo |
| Author_xml | – sequence: 1 givenname: Hidenori surname: Hayashi fullname: Hayashi, Hidenori organization: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Saitama 351-0198, Japan 2Departments of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan 3Department of Medicine, Shiga Health Insurance Hospital, Otsu, Shiga 520-0846, Japan – sequence: 2 givenname: Rei surname: Takahashi fullname: Takahashi, Rei organization: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Saitama 351-0198, Japan 2Departments of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan 3Department of Medicine, Shiga Health Insurance Hospital, Otsu, Shiga 520-0846, Japan – sequence: 3 givenname: Takahiro surname: Nishi fullname: Nishi, Takahiro organization: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Saitama 351-0198, Japan 2Departments of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan 3Department of Medicine, Shiga Health Insurance Hospital, Otsu, Shiga 520-0846, Japan – sequence: 4 givenname: Mitsuo surname: Sakamoto fullname: Sakamoto, Mitsuo organization: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Saitama 351-0198, Japan 2Departments of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan 3Department of Medicine, Shiga Health Insurance Hospital, Otsu, Shiga 520-0846, Japan – sequence: 5 givenname: Yoshimi surname: Benno fullname: Benno, Yoshimi organization: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Saitama 351-0198, Japan 2Departments of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan 3Department of Medicine, Shiga Health Insurance Hospital, Otsu, Shiga 520-0846, Japan |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/16192442$$D View this record in MEDLINE/PubMed |
| BookMark | eNo1kLlOAzEQhl0EkQNKWuSKig1er9eJyyjikjgkjjryescbRz6CvVvkoXhHzFX9mtGnb0b_FI188IDQWUnmJRHiaufcnMxZLaq6ICM0IYTSgvKyHqNpSjtCykVViWM0LnkpKGN0gj4fgwU1WBmx9NIekkk4aLyD3ZDHS2wsfIeSoKTNSIsjqD4UyXQumDbvtoOTHqtggzcKO6NiaEzoJR6S8R0u-SuOL08r3IEHbE0TZTSQflQ9RGfymexMfTSqN8FjHWXnwPfYgu_6Ld4He3Ah7rcmuRN0pKVNcPqXM_R-c_22visenm_v16uHQlVL2hcLWOhlJaHSnLZNW1YNUMEoI0sCtcj1MLrQQpOa0KZuhOZC8lwVE7ohNW81naGLX-8-ho8hP7dxJimwVnoIQ9rwJWeM1yyD53_g0DhoN_tonIyHzX_B9AuycYBG |
| CitedBy_id | crossref_primary_10_14309_ajg_0000000000000834 crossref_primary_10_1007_DCR_0b013e3181a7b77a crossref_primary_10_3389_fmicb_2020_618106 crossref_primary_10_1080_00365520701204238 crossref_primary_10_3945_jn_116_237404 crossref_primary_10_1371_journal_pone_0170050 crossref_primary_10_1128_mra_01217_23 crossref_primary_10_1371_journal_pone_0087560 crossref_primary_10_20538_1682_0363_2015_3_49_62 crossref_primary_10_1155_2017_1890512 crossref_primary_10_1007_s00441_016_2476_9 crossref_primary_10_1053_j_gastro_2019_07_007 crossref_primary_10_1016_j_csbj_2020_12_008 crossref_primary_10_1016_j_biochi_2020_09_007 crossref_primary_10_2217_fmb_13_66 crossref_primary_10_1016_j_bpg_2017_09_010 crossref_primary_10_3389_fcimb_2020_00151 crossref_primary_10_1111_j_1348_0421_2008_00048_x crossref_primary_10_1136_gutjnl_2011_301955 crossref_primary_10_3390_nu3060637 crossref_primary_10_1128_AEM_00753_08 crossref_primary_10_1111_1541_4337_12401 crossref_primary_10_1128_JCM_01913_10 crossref_primary_10_1016_j_pdpdt_2019_05_042 crossref_primary_10_1038_s41598_020_77282_w crossref_primary_10_1111_j_1365_2672_2006_03106_x crossref_primary_10_1186_s40168_020_0785_4 crossref_primary_10_1586_erm_09_16 crossref_primary_10_1016_j_fsigen_2023_102904 crossref_primary_10_1111_1574_6941_12184 crossref_primary_10_3390_brainsci10060384 crossref_primary_10_3389_fmicb_2024_1485073 crossref_primary_10_1146_annurev_micro_090110_102830 crossref_primary_10_1111_j_1740_0929_2007_00494_x crossref_primary_10_3389_fphar_2017_00387 crossref_primary_10_3389_fimmu_2019_02950 crossref_primary_10_1210_jc_2015_4251 crossref_primary_10_1111_mec_12742 crossref_primary_10_1016_j_esmogo_2025_100229 crossref_primary_10_1016_j_surg_2008_12_003 crossref_primary_10_1007_s00253_020_10922_3 crossref_primary_10_1038_s41575_024_01000_4 crossref_primary_10_1097_MOG_0b013e3282f2b0e8 crossref_primary_10_3389_fmicb_2022_1054061 crossref_primary_10_1016_j_jff_2019_04_006 crossref_primary_10_1158_1078_0432_CCR_15_0527 crossref_primary_10_31146_1682_8658_ecg_188_4_178_187 crossref_primary_10_1038_ismej_2011_212 crossref_primary_10_1128_mSphere_00126_19 crossref_primary_10_1371_journal_pone_0163720 crossref_primary_10_1093_procel_pwae058 crossref_primary_10_1017_S0007114510003363 crossref_primary_10_1016_j_phrs_2012_09_008 crossref_primary_10_1371_journal_pone_0125371 crossref_primary_10_1007_s40495_019_00200_w crossref_primary_10_1093_femsle_fny231 crossref_primary_10_1096_fj_201801848R crossref_primary_10_3389_fnagi_2022_976316 crossref_primary_10_1007_s00535_010_0368_4 crossref_primary_10_1007_s00335_009_9204_7 crossref_primary_10_1111_mec_14905 crossref_primary_10_1016_j_smim_2006_09_001 crossref_primary_10_1007_s40519_018_0499_9 crossref_primary_10_1038_ctg_2016_53 crossref_primary_10_1099_jmm_0_47321_0 crossref_primary_10_1177_0022034509346549 crossref_primary_10_1038_s41396_019_0549_y crossref_primary_10_1186_s13073_020_00764_z crossref_primary_10_1016_j_jaci_2011_10_025 crossref_primary_10_1002_ibd_21751 crossref_primary_10_1016_j_tvjl_2015_03_018 crossref_primary_10_1016_j_gtc_2012_08_001 crossref_primary_10_3390_medicina54060098 crossref_primary_10_3389_fimmu_2020_600886 crossref_primary_10_1128_AEM_03132_09 crossref_primary_10_1007_s00203_023_03589_7 crossref_primary_10_1186_s12876_020_01366_0 crossref_primary_10_1093_jb_mvv021 crossref_primary_10_1111_j_1574_6941_2011_01176_x crossref_primary_10_1155_2017_5604727 crossref_primary_10_3748_wjg_v29_i2_332 crossref_primary_10_1007_s10156_013_0583_z crossref_primary_10_1038_srep40248 crossref_primary_10_1111_j_1462_2920_2010_02294_x crossref_primary_10_1002_mbo3_1020 crossref_primary_10_1128_aem_00196_25 crossref_primary_10_1111_1574_6968_12103 crossref_primary_10_1021_acsami_4c20961 crossref_primary_10_1080_09637486_2021_1906212 crossref_primary_10_3390_ijms24043265 crossref_primary_10_3390_ijms25105561 crossref_primary_10_1128_AEM_05391_11 crossref_primary_10_2217_17460913_2_3_285 crossref_primary_10_1002_ar_24124 crossref_primary_10_1371_journal_pone_0077660 crossref_primary_10_1007_s11894_019_0717_2 crossref_primary_10_3390_ani14182729 crossref_primary_10_1093_femsec_fiz096 crossref_primary_10_1128_MMBR_00014_10 crossref_primary_10_3389_fphar_2021_643283 crossref_primary_10_1128_AEM_00151_06 crossref_primary_10_1146_annurev_food_022510_133640 crossref_primary_10_1186_s12866_019_1669_2 crossref_primary_10_4103_ijmr_IJMR_590_16 crossref_primary_10_1128_MMBR_00008_19 crossref_primary_10_1182_blood_2011_12_401166 crossref_primary_10_1186_1757_4749_5_35 crossref_primary_10_1016_j_isci_2024_111319 crossref_primary_10_1096_fj_10_156257 crossref_primary_10_1016_j_semcancer_2021_09_016 crossref_primary_10_1155_2018_4178607 crossref_primary_10_1038_s41698_025_00873_1 crossref_primary_10_3389_fimmu_2019_02653 crossref_primary_10_1097_MOG_0000000000000040 crossref_primary_10_1093_femsmc_xtae022 crossref_primary_10_1371_journal_pone_0148887 crossref_primary_10_1016_j_dci_2009_09_002 crossref_primary_10_1093_jn_nxab113 crossref_primary_10_4061_2010_321426 crossref_primary_10_1371_journal_pone_0281625 crossref_primary_10_1038_mi_2017_64 crossref_primary_10_1038_nrmicro1840 crossref_primary_10_1093_femsre_fuad022 crossref_primary_10_1371_journal_pone_0055555 crossref_primary_10_2217_fmb_09_82 crossref_primary_10_1111_j_1600_6143_2011_03860_x crossref_primary_10_1186_1471_2180_6_81 crossref_primary_10_1016_j_csbj_2021_08_020 crossref_primary_10_3389_fimmu_2021_798552 crossref_primary_10_3389_fimmu_2023_1242242 crossref_primary_10_1017_S0007114512002279 crossref_primary_10_1111_j_1365_2036_2008_03860_x crossref_primary_10_1111_jam_15684 crossref_primary_10_4315_JFP_20_154 crossref_primary_10_1016_j_scijus_2021_06_006 crossref_primary_10_3390_microorganisms10122424 crossref_primary_10_1007_s10620_020_06118_4 crossref_primary_10_1002_mnfr_202000455 crossref_primary_10_1128_AEM_01902_17 crossref_primary_10_1021_es8006029 crossref_primary_10_1016_j_metabol_2023_155712 crossref_primary_10_1038_s41598_025_07932_4 crossref_primary_10_1016_j_meegid_2014_10_001 crossref_primary_10_1007_s12602_012_9111_1 crossref_primary_10_1128_CMR_00323_20 crossref_primary_10_1007_s00248_015_0645_0 crossref_primary_10_1128_IAI_01496_06 crossref_primary_10_1111_bph_13428 crossref_primary_10_1146_annurev_ecolsys_110411_160307 crossref_primary_10_1111_j_1574_6968_2008_01442_x crossref_primary_10_1007_s00394_015_0852_y crossref_primary_10_1371_journal_pone_0060007 crossref_primary_10_1016_j_trsl_2012_05_003 crossref_primary_10_1093_femsmc_xtae027 crossref_primary_10_6061_clinics_2017_03_05 crossref_primary_10_3390_ijms24119471 crossref_primary_10_12688_wellcomeopenres_15639_1 crossref_primary_10_1002_ibd_20151 crossref_primary_10_1038_ismej_2009_36 crossref_primary_10_12688_wellcomeopenres_15639_2 crossref_primary_10_1002_ibd_20392 crossref_primary_10_1128_mmbr_00127_22 crossref_primary_10_1016_j_coi_2015_07_005 crossref_primary_10_1128_IAI_00268_13 crossref_primary_10_1186_s40168_023_01491_4 crossref_primary_10_1007_s12263_011_0229_7 crossref_primary_10_1186_1471_2334_13_130 crossref_primary_10_1016_j_cmi_2020_08_021 crossref_primary_10_1007_s00253_013_4962_2 crossref_primary_10_1080_14787210_2019_1607297 crossref_primary_10_1080_19490976_2025_2514144 crossref_primary_10_1111_j_1365_2567_2012_03616_x crossref_primary_10_1155_2008_597603 crossref_primary_10_3390_nu10101421 crossref_primary_10_7554_eLife_73552 crossref_primary_10_1258_la_2010_010058 crossref_primary_10_1007_s00203_017_1459_x crossref_primary_10_1017_S0031182014000699 crossref_primary_10_1038_ismej_2013_185 crossref_primary_10_1016_j_alcohol_2013_01_003 crossref_primary_10_3389_fphar_2023_1130562 crossref_primary_10_1111_j_1574_6941_2007_00281_x crossref_primary_10_1038_s41598_017_03066_4 crossref_primary_10_3390_nu14153129 crossref_primary_10_1016_j_cellsig_2023_111013 crossref_primary_10_1111_tid_12423 crossref_primary_10_1134_S0026261708030016 crossref_primary_10_1128_JB_01689_09 crossref_primary_10_1111_iwj_70514 crossref_primary_10_1016_j_mehy_2017_04_011 crossref_primary_10_1038_s41598_022_05723_9 crossref_primary_10_1111_1462_2920_13853 crossref_primary_10_1016_j_mimet_2013_05_008 crossref_primary_10_1016_j_envpol_2017_10_129 crossref_primary_10_1128_JB_00177_21 crossref_primary_10_3389_fmicb_2020_00496 crossref_primary_10_1128_AEM_01807_13 crossref_primary_10_1016_j_tree_2006_06_013 crossref_primary_10_1186_s13578_025_01385_y crossref_primary_10_1093_femsre_fuaa015 crossref_primary_10_1128_jb_00056_25 crossref_primary_10_3389_fimmu_2022_845243 crossref_primary_10_1016_j_mrfmmm_2007_03_011 crossref_primary_10_1002_1873_3468_12421 crossref_primary_10_1007_s00248_014_0381_x crossref_primary_10_1159_000524023 crossref_primary_10_1155_2021_1391384 crossref_primary_10_1038_s41598_018_23198_5 crossref_primary_10_1007_s12328_009_0106_8 crossref_primary_10_1007_s00284_008_9345_z crossref_primary_10_1016_j_amjsurg_2014_10_028 |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1099/jmm.0.45935-0 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Medicine Biology |
| ExternalDocumentID | 16192442 |
| Genre | Research Support, Non-U.S. Gov't Journal Article Comparative Study |
| GroupedDBID | --- -~X .55 .GJ 08G 18M 1KJ 2WC 3O- 4.4 53G 5GY 5RE 5RS 5VS AAKAS AAYEP ABOCM ACBNA ACGFO ACPEE ADBBV ADCDP ADZCM AENEX AFFNX AFOSN AI. AJKYU ALMA_UNASSIGNED_HOLDINGS AWKKM BAWUL C45 CAG CGR COF CS3 CUY CVF DIK DU5 E3Z EBS ECM EIF EJD EX3 F5P GX1 H13 L7B M5~ NPM NTWIH OBC OBS OEB OFXIZ OHT OK1 OVD OWW P0W P2P PKN RGM TEORI TR2 VH1 VVN W8F WOQ WOW X7M YFH YQJ YQY ZE2 ZGI ZXP 7X8 ABUFD |
| ID | FETCH-LOGICAL-c382t-7e7f83ae3f62dbd13be29424080e59593427f9f0502b5b9f69a602249fb056df2 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 266 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000233091400014&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 0022-2615 |
| IngestDate | Sun Nov 09 14:11:00 EST 2025 Wed Feb 19 02:31:18 EST 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | Pt 11 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c382t-7e7f83ae3f62dbd13be29424080e59593427f9f0502b5b9f69a602249fb056df2 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
| PMID | 16192442 |
| PQID | 68644654 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_68644654 pubmed_primary_16192442 |
| PublicationCentury | 2000 |
| PublicationDate | 2005-11-01 |
| PublicationDateYYYYMMDD | 2005-11-01 |
| PublicationDate_xml | – month: 11 year: 2005 text: 2005-11-01 day: 01 |
| PublicationDecade | 2000 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England |
| PublicationTitle | Journal of medical microbiology |
| PublicationTitleAlternate | J Med Microbiol |
| PublicationYear | 2005 |
| SSID | ssj0017339 |
| Score | 2.2904668 |
| Snippet | Microbiota in gut contents of jejunum, ileum, caecum and recto-sigmoid colon obtained from three elderly individuals at autopsy were compared using 16S rRNA... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 1093 |
| SubjectTerms | Aged Bacteria - classification Bacteria - genetics Cecum - microbiology Colon, Sigmoid - microbiology Deoxyribonuclease HpaII - metabolism Deoxyribonucleases, Type II Site-Specific - metabolism DNA Fingerprinting DNA, Bacterial - analysis DNA, Bacterial - chemistry DNA, Bacterial - isolation & purification DNA, Ribosomal - genetics Female Humans Ileum - microbiology Intestines - microbiology Jejunum - microbiology Male Molecular Sequence Data Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA |
| Title | Molecular analysis of jejunal, ileal, caecal and recto-sigmoidal human colonic microbiota using 16S rRNA gene libraries and terminal restriction fragment length polymorphism |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/16192442 https://www.proquest.com/docview/68644654 |
| Volume | 54 |
| WOSCitedRecordID | wos000233091400014&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwEB4VWhAXoC2P5VHm0GPTZu28LCGhClFx6K4qaKu9rZzYXrJqkmUfSP1R_EdmnISeKg5cEiWKoygzmXwzY38fwGEick0_AkfITXG1SmSBNpEIIqtcHpuwkJ7A9Po8HY-zyURdbMHHfi0MT6vsY6IP1KYpuEZ-kmSJ5_76tPgZsGYU91Y7AY0HsC0JyLBPp5O7HkIqpeq5wilPiDuGTYJEJ_OqOg6Po1jJOAjvx5b-H3P27P-e7jk87bAlnrbOsAtbtt6DR63a5O0ePB51ffR9-D3qVXFRd6wk2Dic2_mGDo-QQgXvCm3JhHSJQQ6MTbAqZ1VTGjrntf2QKa_rssCqbPmc1hp5Iv0Mh8l3XH4bnyL5p8W_Obm_VTf_5gZZFmRZ-oUV6JZ6xoVKZGWX9Q9cNDe3VUNOUK6qF3B19uXy89egE24ICpmJdZDa1GVSW-kSYXIzlLkVKmIytdDGzIQcidQpF8ahyONcuUTphLEEuQfhMePES3hYN7V9DajS2NGb1Yl0hC1pLEVvGjI0qeTkqhjAh94gU_owuNuha9tsVtPeJAN41dp0umj5O6ZDn3VG4s0_x76FJy1VK5dc3sG2o5Bg38NO8WtdrpYH3t9oO74Y_QE3puGr |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Molecular+analysis+of+jejunal%2C+ileal%2C+caecal+and+recto-sigmoidal+human+colonic+microbiota+using+16S+rRNA+gene+libraries+and+terminal+restriction+fragment+length+polymorphism&rft.jtitle=Journal+of+medical+microbiology&rft.au=Hayashi%2C+Hidenori&rft.au=Takahashi%2C+Rei&rft.au=Nishi%2C+Takahiro&rft.au=Sakamoto%2C+Mitsuo&rft.date=2005-11-01&rft.issn=0022-2615&rft.volume=54&rft.issue=Pt+11&rft.spage=1093&rft_id=info:doi/10.1099%2Fjmm.0.45935-0&rft_id=info%3Apmid%2F16192442&rft_id=info%3Apmid%2F16192442&rft.externalDocID=16192442 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0022-2615&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0022-2615&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0022-2615&client=summon |