Application of solid-phase microextraction to in vitro skin permeation experiments: example using diethyl phthalate

The application of automated solid-phase microextraction (SPME) as a sample preparation technique for in vitro studies of skin permeation is described, using diethyl phthalate (DEP) as an example. In vitro diffusion cell experiments and skin–vehicle partition coefficient determinations require quant...

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Bibliographic Details
Published in:Toxicology in vitro Vol. 19; no. 2; pp. 253 - 259
Main Authors: Frasch, H. Fred, Barbero, Ana M.
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01.03.2005
Subjects:
Animals
Chromatography, Gas
Diffusion
Environmental Pollutants - metabolism
Gas chromatography methods
Guinea Pigs
Male
Membrane vehicle partition coefficient
Microchemistry - methods
Organ Culture Techniques
Permeability
Phthalic acid diesters
Phthalic Acids - metabolism
Reproducibility of Results
Sensitivity and Specificity
Skin Absorption - physiology
Specimen Handling - methods
Time lag
Membrane vehicle partition coefficient
Gas chromatography methods
Permeability
Phthalic acid diesters
Time lag
ISSN:0887-2333, 1879-3177
Online Access:Get full text
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Summary:The application of automated solid-phase microextraction (SPME) as a sample preparation technique for in vitro studies of skin permeation is described, using diethyl phthalate (DEP) as an example. In vitro diffusion cell experiments and skin–vehicle partition coefficient determinations require quantitative analysis of low-level analytes in aqueous samples. SPME is an ideal candidate for sample preparation for subsequent gas chromatographic analysis, offering numerous advantages over other methods. SPME conditions were optimized and the automated method was found to exhibit adequate sensitivity and good precision (relative standard deviation = 3%). Abdominal skin (dermatomed at 350 μm) from male hairless guinea pigs ( n = 6) was used to measure DEP skin permeation parameters. In vitro methods were employed to determine permeability coefficient ( k p), time lag ( τ) and skin–buffer partition coefficient ( K SB) for 2 mM DEP in HEPES buffered Hanks Balanced Salt Solution. Measurements (mean ± standard deviations) are: k p, 0.021 ± 0.012 cm/h; τ, 0.67 ± 0.18 h; K SB, 4.74 ± 0.68. The skin may be a significant route for the uptake of DEP.
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ISSN:0887-2333
1879-3177
DOI:10.1016/j.tiv.2004.10.001