Gene expression profiling of in vitro cultured macrophages after exposure to the respiratory sensitizer hexamethylene diisocyanate

Occupational exposure to chemicals is one of the main causes of respiratory allergy and asthma. Identification of chemicals that trigger allergic asthma is difficult as underlying processes and specific markers have not yet been clearly defined. Moreover, adequate classification of the respiratory t...

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Bibliographic Details
Published in:Toxicology in vitro Vol. 22; no. 4; pp. 1107 - 1114
Main Authors: Verstraelen, S., Wens, B., Hooyberghs, J., Nelissen, I., Witters, H., Schoeters, G., Cauwenberge, P. Van, Heuvel, R. Van Den
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01.06.2008
Subjects:
Air Pollutants, Occupational - toxicity
Cell Differentiation
Cell Line
Cell Line, Tumor
Cells, Cultured
Cyanates - toxicity
Gene Expression Profiling
Gene Expression Regulation - drug effects
Hexamethylene diisocyanate
Humans
Isocyanates
Leukemia, Monocytic, Acute
Macrophages
Occupational Exposure
Oligonucleotide Array Sequence Analysis
Respiratory allergy
Time Factors
Transcriptomics
Hexamethylene diisocyanate
IVT
DNCB
HDI
Macrophages
IPDI
BT
OA
MDI
Transcriptomics
TDI
HSC
IC
GSH
NiSO4
HSP
PMA
pPD
Respiratory allergy
GO
aRNA
CCL
aaUTP
Ig
SAM
DC
ISSN:0887-2333, 1879-3177
Online Access:Get full text
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Summary:Occupational exposure to chemicals is one of the main causes of respiratory allergy and asthma. Identification of chemicals that trigger allergic asthma is difficult as underlying processes and specific markers have not yet been clearly defined. Moreover, adequate classification of the respiratory toxicity of chemicals is hampered due to the lack of validated in vivo and in vitro test methods. The study of differential gene expression profiles in appropriate human in vitro cell systems is a promising approach to identify selective markers for respiratory allergy. As alveolar macrophages display important immunological and inflammatory properties in response to foreign substances in the lung, we aimed at gaining more insight in changes of human macrophages transcriptome and to identify selective genetic markers for respiratory sensitization in response to hexamethylene diisocyanate (HDI). In vitro cultures of human THP-1 cells were differentiated into macrophages and exposed to 55μg/ml HDI for 6 and 10h. Using human oligonucleotide microarrays, changes were observed in the expression of genes that are involved in diverse biological and molecular processes, including detoxification, oxidative stress, cytokine signaling, and apoptosis, which can lead to the development of asthma. These genes are possible markers for respiratory sensitization caused by isocyanates.
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ISSN:0887-2333
1879-3177
DOI:10.1016/j.tiv.2008.02.015