Reappraisal of in situ immunophenotypic analysis of psoriasis skin: interaction of activated HLA-DR+ immunocompetent cells and endothelial cells is a major feature of psoriatic lesions
Psoriasis is an inflammatory skin disease of unknown aetiology. Many observations indicate that T cells play an important role in the pathogenesis of the disease. Upregulation of MHC class-II molecules on immunocompetent cells, endothelial cells and keratinocytes on lesional psoriatic skin has been...
Gespeichert in:
| Veröffentlicht in: | Archives of dermatological research Jg. 286; H. 2; S. 87 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Journal Article |
| Sprache: | Englisch |
| Veröffentlicht: |
Germany
01.02.1994
|
| Schlagworte: | |
| ISSN: | 0340-3696 |
| Online-Zugang: | Weitere Angaben |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Abstract | Psoriasis is an inflammatory skin disease of unknown aetiology. Many observations indicate that T cells play an important role in the pathogenesis of the disease. Upregulation of MHC class-II molecules on immunocompetent cells, endothelial cells and keratinocytes on lesional psoriatic skin has been regarded as a hallmark of the disease. However, there is some controversy in the literature regarding the cell types expressing class-II molecules and there is limited information about the presence of immune cells other than T cells and antigen presenting cells in the cellular infiltrates of psoriatic skin. We therefore reinvestigated the subject using immunocytochemical single and multiple staining techniques. In agreement with earlier reports, our studies showed that the cellular infiltrates in lesional skin consist largely of HLA-DR+/IL-2R+ T cells, HLA-DR+/CD1a+ Langerhans cells, and HLA-DR+/CD68+ macrophages. We found increased HLA-DR expression mostly on immuno-competent cells and endothelial cells, but no prominent HLA-DR expression on keratinocytes in lesional psoriatic skin. Upregulation of HLA-DR on endothelial cells and in mononuclear infiltrates was also evident in the non-lesional skin of psoriatic patients as compared with normal controls. B cells and natural killer cells were also found in the cellular infiltrates in lesional psoriatic skin. In spite of the presence of a large amount of activated T cells in the epidermis, we found that HLA-DR expression on keratinocytes was not a major feature of psoriatic skin. |
|---|---|
| AbstractList | Psoriasis is an inflammatory skin disease of unknown aetiology. Many observations indicate that T cells play an important role in the pathogenesis of the disease. Upregulation of MHC class-II molecules on immunocompetent cells, endothelial cells and keratinocytes on lesional psoriatic skin has been regarded as a hallmark of the disease. However, there is some controversy in the literature regarding the cell types expressing class-II molecules and there is limited information about the presence of immune cells other than T cells and antigen presenting cells in the cellular infiltrates of psoriatic skin. We therefore reinvestigated the subject using immunocytochemical single and multiple staining techniques. In agreement with earlier reports, our studies showed that the cellular infiltrates in lesional skin consist largely of HLA-DR+/IL-2R+ T cells, HLA-DR+/CD1a+ Langerhans cells, and HLA-DR+/CD68+ macrophages. We found increased HLA-DR expression mostly on immuno-competent cells and endothelial cells, but no prominent HLA-DR expression on keratinocytes in lesional psoriatic skin. Upregulation of HLA-DR on endothelial cells and in mononuclear infiltrates was also evident in the non-lesional skin of psoriatic patients as compared with normal controls. B cells and natural killer cells were also found in the cellular infiltrates in lesional psoriatic skin. In spite of the presence of a large amount of activated T cells in the epidermis, we found that HLA-DR expression on keratinocytes was not a major feature of psoriatic skin.Psoriasis is an inflammatory skin disease of unknown aetiology. Many observations indicate that T cells play an important role in the pathogenesis of the disease. Upregulation of MHC class-II molecules on immunocompetent cells, endothelial cells and keratinocytes on lesional psoriatic skin has been regarded as a hallmark of the disease. However, there is some controversy in the literature regarding the cell types expressing class-II molecules and there is limited information about the presence of immune cells other than T cells and antigen presenting cells in the cellular infiltrates of psoriatic skin. We therefore reinvestigated the subject using immunocytochemical single and multiple staining techniques. In agreement with earlier reports, our studies showed that the cellular infiltrates in lesional skin consist largely of HLA-DR+/IL-2R+ T cells, HLA-DR+/CD1a+ Langerhans cells, and HLA-DR+/CD68+ macrophages. We found increased HLA-DR expression mostly on immuno-competent cells and endothelial cells, but no prominent HLA-DR expression on keratinocytes in lesional psoriatic skin. Upregulation of HLA-DR on endothelial cells and in mononuclear infiltrates was also evident in the non-lesional skin of psoriatic patients as compared with normal controls. B cells and natural killer cells were also found in the cellular infiltrates in lesional psoriatic skin. In spite of the presence of a large amount of activated T cells in the epidermis, we found that HLA-DR expression on keratinocytes was not a major feature of psoriatic skin. Psoriasis is an inflammatory skin disease of unknown aetiology. Many observations indicate that T cells play an important role in the pathogenesis of the disease. Upregulation of MHC class-II molecules on immunocompetent cells, endothelial cells and keratinocytes on lesional psoriatic skin has been regarded as a hallmark of the disease. However, there is some controversy in the literature regarding the cell types expressing class-II molecules and there is limited information about the presence of immune cells other than T cells and antigen presenting cells in the cellular infiltrates of psoriatic skin. We therefore reinvestigated the subject using immunocytochemical single and multiple staining techniques. In agreement with earlier reports, our studies showed that the cellular infiltrates in lesional skin consist largely of HLA-DR+/IL-2R+ T cells, HLA-DR+/CD1a+ Langerhans cells, and HLA-DR+/CD68+ macrophages. We found increased HLA-DR expression mostly on immuno-competent cells and endothelial cells, but no prominent HLA-DR expression on keratinocytes in lesional psoriatic skin. Upregulation of HLA-DR on endothelial cells and in mononuclear infiltrates was also evident in the non-lesional skin of psoriatic patients as compared with normal controls. B cells and natural killer cells were also found in the cellular infiltrates in lesional psoriatic skin. In spite of the presence of a large amount of activated T cells in the epidermis, we found that HLA-DR expression on keratinocytes was not a major feature of psoriatic skin. |
| Author | de Boer, O J Hamerlinck, F Das, P K van der Loos, C M Bos, J D |
| Author_xml | – sequence: 1 givenname: O J surname: de Boer fullname: de Boer, O J organization: Department of Dermatology, University of Amsterdam, The Netherlands – sequence: 2 givenname: C M surname: van der Loos fullname: van der Loos, C M – sequence: 3 givenname: F surname: Hamerlinck fullname: Hamerlinck, F – sequence: 4 givenname: J D surname: Bos fullname: Bos, J D – sequence: 5 givenname: P K surname: Das fullname: Das, P K |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/7512323$$D View this record in MEDLINE/PubMed |
| BookMark | eNpFUE1r3DAQ1SEl2Sa55B7QqZfidCzZltXbkq8tLBRCcl7G8phoY0uuJRf2n-XnRSYLGRjm6817w3xnJ847Yuwqh5scQP1qOgCpQEl5wlYgC8hkpaszdhnCHpIpKASoU3aqylxIIVfs_YlwHCe0AXvuO24dDzbO3A7D7Pz4Ss7Hw2gNR4f9IdiwgMbgJ4tLEd6s-52WIk1oovVuGS_Zf4zU8s12nd09_TyyGT-MFMlFbqjvQ6JsObnWx1fqbZL_7CZW5APu_cQ7wjhP9CUZ0yE9haQTLti3DvtAl8d4zl4e7p9vN9n27-Of2_U2M1KpmNV1XpWFEkVN2tStrkAYDa3UqlQlYJHXjSpJExjokuumKXMUUBlCXQmN4pz9-OQdJ_9vphB3gw3LpejIz2GnqkJUWkICXh-BczNQuxsnO-B02B1fLT4AS5KDsQ |
| CitedBy_id | crossref_primary_10_1177_039463209801100207 crossref_primary_10_1046_j_1523_1747_1998_00419_x crossref_primary_10_1046_j_1365_2133_2001_04143_x crossref_primary_10_1016_j_jid_2022_05_1089 crossref_primary_10_1177_014107689608900604 crossref_primary_10_3892_mmr_2018_9177 crossref_primary_10_1016_j_cca_2008_04_005 crossref_primary_10_1016_j_jid_2022_01_014 crossref_primary_10_1016_S0733_8635_18_30038_X crossref_primary_10_1016_S0165_2478_02_00223_7 crossref_primary_10_1016_j_autrev_2006_03_012 crossref_primary_10_1111_j_1365_2133_2007_08039_x crossref_primary_10_1016_j_jdermsci_2007_06_014 crossref_primary_10_1016_S0167_5699_98_01381_4 crossref_primary_10_1016_j_jaci_2020_11_028 crossref_primary_10_1016_0738_081X_95_93818_9 crossref_primary_10_1177_120347549700100313 crossref_primary_10_1086_301899 crossref_primary_10_1177_120347549800300113 crossref_primary_10_1006_jaut_1999_0343 crossref_primary_10_1023_A_1024584732614 |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1007/bf00370733 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Medicine |
| ExternalDocumentID | 7512323 |
| Genre | Journal Article |
| GroupedDBID | --- -53 -5E -5G -BR -EM -Y2 -~C .55 .86 .VR 06C 06D 0R~ 0VY 199 1N0 1SB 2.D 203 23M 28- 29~ 2J2 2JN 2JY 2KG 2KM 2LR 2P1 2VQ 2~H 30V 36B 3V. 406 408 409 40D 40E 53G 5QI 5VS 67Z 6NX 78A 7X7 88E 8AO 8FI 8FJ 8FW 8TC 8UJ 95- 95. 95~ 96X AAAVM AABHQ AACDK AAHNG AAIAL AAJBT AAJKR AANXM AANZL AARHV AARTL AASML AATNV AATVU AAUYE AAWCG AAYIU AAYQN AAYTO AAYZH ABAKF ABBBX ABBXA ABDZT ABECU ABFTV ABHLI ABHQN ABIPD ABJNI ABJOX ABKCH ABKTR ABLJU ABMNI ABMQK ABNWP ABPLI ABQBU ABQSL ABSXP ABTEG ABTKH ABTMW ABULA ABUWG ABWNU ABXPI ACAOD ACBXY ACDTI ACGFS ACHSB ACHXU ACKNC ACMDZ ACMLO ACOKC ACOMO ACPIV ACPRK ACUDM ACZOJ ADBBV ADHHG ADHIR ADIMF ADINQ ADJJI ADKNI ADKPE ADRFC ADTPH ADURQ ADYFF ADZKW AEBTG AEFIE AEFQL AEGAL AEGNC AEJHL AEJRE AEKMD AEMSY AENEX AEOHA AEPYU AESKC AETLH AEVLU AEXYK AFBBN AFEXP AFKRA AFLOW AFQWF AFWTZ AFZKB AGAYW AGDGC AGGDS AGJBK AGMZJ AGQEE AGQMX AGRTI AGWIL AGWZB AGYKE AHAVH AHBYD AHIZS AHMBA AHSBF AHYZX AIAKS AIGIU AIIXL AILAN AITGF AJBLW AJRNO AJZVZ AKMHD ALIPV ALMA_UNASSIGNED_HOLDINGS ALWAN AMKLP AMXSW AMYLF AMYQR AOCGG ARMRJ AXYYD AZFZN B-. BA0 BBWZM BDATZ BENPR BGNMA BPHCQ BSONS BVXVI CAG CCPQU CGR COF CSCUP CUY CVF DDRTE DL5 DNIVK DPUIP EBD EBLON EBS ECM EIF EIOEI EJD EMB EMOBN EN4 ESBYG F5P FEDTE FERAY FFXSO FIGPU FINBP FNLPD FRRFC FSGXE FWDCC FYUFA G-Y G-Z GGCAI GGRSB GJIRD GNWQR GQ6 GQ7 GQ8 GRRUI GXS H13 HF~ HG5 HG6 HMCUK HMJXF HQYDN HRMNR HVGLF HZ~ I09 IHE IJ- IKXTQ IMOTQ IWAJR IXC IXD IXE IZIGR IZQ I~X I~Z J-C J0Z JBSCW JCJTX KDC KOV KOW KPH LAS LLZTM M1P M4Y MA- N2Q N9A NB0 NDZJH NPM NPVJJ NQJWS NU0 O9- O93 O9G O9I O9J OAM OVD P19 P2P P9S PF0 PQQKQ PROAC PSQYO PT4 PT5 Q2X QOK QOR QOS R89 R9I RHV RIG RNI ROL RPX RRX RSV RZK S16 S1Z S26 S27 S28 S37 S3B SAP SCLPG SDE SDH SDM SHX SISQX SJYHP SMD SNE SNPRN SNX SOHCF SOJ SPISZ SRMVM SSLCW SSXJD STPWE SV3 SZ9 SZN T13 T16 TEORI TSG TSK TSV TT1 TUC U2A U9L UG4 UKHRP UOJIU UTJUX UZXMN VC2 VFIZW W23 W48 WJK WK8 X7M Y6R YLTOR Z45 Z7U Z7V Z7W Z82 Z87 Z8O Z8P Z8Q Z8V Z91 ZGI ZMTXR ZOVNA ~EX ~KM 7X8 ADHKG AGQPQ ATHPR |
| ID | FETCH-LOGICAL-c377t-8816547248e9c8d9602c90d3975750a418b75e9e0c0f0c09bb51a206cea9629a2 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 32 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=10_1007_BF00370733&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 0340-3696 |
| IngestDate | Thu Sep 04 15:28:01 EDT 2025 Wed Feb 19 02:35:06 EST 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c377t-8816547248e9c8d9602c90d3975750a418b75e9e0c0f0c09bb51a206cea9629a2 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 7512323 |
| PQID | 76426930 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_76426930 pubmed_primary_7512323 |
| PublicationCentury | 1900 |
| PublicationDate | 1994-02-01 |
| PublicationDateYYYYMMDD | 1994-02-01 |
| PublicationDate_xml | – month: 02 year: 1994 text: 1994-02-01 day: 01 |
| PublicationDecade | 1990 |
| PublicationPlace | Germany |
| PublicationPlace_xml | – name: Germany |
| PublicationTitle | Archives of dermatological research |
| PublicationTitleAlternate | Arch Dermatol Res |
| PublicationYear | 1994 |
| SSID | ssj0000704207 ssj0007781 |
| Score | 1.5436867 |
| Snippet | Psoriasis is an inflammatory skin disease of unknown aetiology. Many observations indicate that T cells play an important role in the pathogenesis of the... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 87 |
| SubjectTerms | Adult B-Lymphocytes - immunology Endothelium - immunology Endothelium - pathology Female HLA-DR Antigens - analysis Humans Immunohistochemistry Immunophenotyping Keratinocytes - immunology Killer Cells, Natural - immunology Langerhans Cells - immunology Major Histocompatibility Complex - immunology Male Psoriasis - immunology Psoriasis - pathology Skin - immunology Skin - pathology Staining and Labeling T-Lymphocytes - immunology |
| Title | Reappraisal of in situ immunophenotypic analysis of psoriasis skin: interaction of activated HLA-DR+ immunocompetent cells and endothelial cells is a major feature of psoriatic lesions |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/7512323 https://www.proquest.com/docview/76426930 |
| Volume | 286 |
| WOSCitedRecordID | wos10_1007_BF00370733&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LT9wwELYoVBWXQmkRlJcPvSGrxnZiGyGhFQ9xgBVCgPa2cmxHCo9ku9lF6j_j5zGTZKk4VBw4JMrDGTvyePyNxzNDyC-XJRbmocCsSxRTXgdmpAsstUpkKkjHG4vp7bnu981gYC_nyMHMFwa3Vc5kYiOoQ-Vxjfy3TtHpUvLD0R-GOaPQttol0PhEFiQAGRyWemBeV1iAmZXgr-oX17pJWcqlAsmT2vRtsFIuNeYv_D_QbCac06WPNXWZfO2AJu21nPGNzMVyhXy56Ezp38nzVcR44q6ooVSV06KkQGFKC3QYwWADZTX5Oyo8dV3YEiw0qqEmhzf1fVHuUww2MW5dI_A1Xj0BeA307LzHjq92O2q-A-cTinaCGkgGGsuAzl8PwP_dU6Dq6KO7q8Y0j0280X9Vwk_Qh4gre_UPcnN6cn10xro0DsxLrSfMGPSY0kKZaL0JoDIJb3kAIARQkTu1ZzKdRBu55zkcNsuSPSd46qOzqbBOrJL5sirjGqHGgUKnghJ5tIpnPMvTKHJQ-qwMUEeyTnZmPTKEYYKtd2WspvVw1ifrZLXt1OGojeYx1AmiSvnz3U83yGIbSBn3smyShRzkQ9win_3TpKjH2w3zwbl_efECnU_lug |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Reappraisal+of+in+situ+immunophenotypic+analysis+of+psoriasis+skin%3A+interaction+of+activated+HLA-DR%2B+immunocompetent+cells+and+endothelial+cells+is+a+major+feature+of+psoriatic+lesions&rft.jtitle=Archives+of+dermatological+research&rft.au=de+Boer%2C+O+J&rft.au=van+der+Loos%2C+C+M&rft.au=Hamerlinck%2C+F&rft.au=Bos%2C+J+D&rft.date=1994-02-01&rft.issn=0340-3696&rft.volume=286&rft.issue=2&rft.spage=87&rft_id=info:doi/10.1007%2FBF00370733&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0340-3696&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0340-3696&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0340-3696&client=summon |