Glucocorticoids promote a TH2 cytokine response by CD4+ T cells in vitro

Purified rat CD4+ T cells were activated in vitro in the presence or absence of the glucocorticoid dexamethasone. They were then expanded in IL-2 and subsequently restimulated, this time in the absence of the hormone. The results indicate that the exposure of the cells to dexamethasone in the primar...

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Bibliographic Details
Published in:The Journal of immunology (1950) Vol. 156; no. 7; p. 2406
Main Authors: Ramírez, F, Fowell, D J, Puklavec, M, Simmonds, S, Mason, D
Format: Journal Article
Language:English
Published: United States 01.04.1996
Subjects:
Animals
Antibodies, Monoclonal - biosynthesis
Base Sequence
CD4-Positive T-Lymphocytes - drug effects
CD4-Positive T-Lymphocytes - immunology
Concanavalin A - pharmacology
Cytokines - biosynthesis
Cytokines - genetics
Dexamethasone - pharmacology
DNA Primers - genetics
Glucocorticoids - pharmacology
In Vitro Techniques
Interferon-gamma - biosynthesis
Interferon-gamma - genetics
Interleukin-10 - biosynthesis
Interleukin-10 - genetics
Interleukin-13 - biosynthesis
Interleukin-13 - genetics
Interleukin-4 - biosynthesis
Interleukin-4 - genetics
Lymphocyte Activation
Mice
Molecular Sequence Data
Rats
RNA, Messenger - genetics
RNA, Messenger - metabolism
Th1 Cells - drug effects
Th1 Cells - immunology
Th2 Cells - drug effects
Th2 Cells - immunology
Tumor Necrosis Factor-alpha - biosynthesis
Tumor Necrosis Factor-alpha - genetics
ISSN:0022-1767
Online Access:Get more information
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Summary:Purified rat CD4+ T cells were activated in vitro in the presence or absence of the glucocorticoid dexamethasone. They were then expanded in IL-2 and subsequently restimulated, this time in the absence of the hormone. The results indicate that the exposure of the cells to dexamethasone in the primary stimulation changed the cytokine synthesis induced by the secondary stimulation. The mRNA levels for IL-4, IL-10, and IL-13 were all increased by the pretreatment, whereas synthesis of IFN-gamma and TNF-alpha was diminished. Further studies in which IL-4 was used together with dexamethasone showed that the cytokine potentiated the effect of the hormone. These data suggest that the neuroendocrine system can influence the cytokine response to pathogens and autoantigens in a way that favors Th2-type reactions. There are similar implications for therapy with glucocorticoids, and these drugs may be expected to have long term immunologic effects as well as short-lived immunosuppressive ones. The production of a mouse mAb, MRC-OX81, against rat IL-4 is also described.
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ISSN:0022-1767
DOI:10.4049/jimmunol.156.7.2406