First identification of infectious salmon anaemia virus in North America with haemorrhagic kidney syndrome

Haemorrhagic kidney syndrome (HKS), a serious disease affecting Atlantic salmon on the east coast of Canada, was determined to be caused by infectious salmon anaemia virus (ISAV) through the isolation of the pathogen on the SHK-1 (salmon head kidney) cell line and confirmation by ISAV-specific immun...

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Veröffentlicht in:Diseases of aquatic organisms Jg. 35; H. 2; S. 145
Hauptverfasser: Lovely, J E, Dannevig, B H, Falk, K, Hutchin, L, MacKinnon, A M, Melville, K J, Rimstad, E, Griffiths, S G
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Germany 29.01.1999
Schlagworte:
Animals
Cell Line
Cytopathogenic Effect, Viral
DNA, Viral - analysis
Fish Diseases - virology
Fluorescent Antibody Technique, Indirect - veterinary
Hemorrhage - veterinary
Hemorrhage - virology
Kidney Diseases - veterinary
Kidney Diseases - virology
New Brunswick
Orthomyxoviridae - genetics
Orthomyxoviridae - isolation & purification
Orthomyxoviridae - pathogenicity
Orthomyxoviridae Infections - veterinary
Orthomyxoviridae Infections - virology
Reverse Transcriptase Polymerase Chain Reaction - veterinary
Salmo salar
Syndrome
Virulence
New Brunswick
ISSN:0177-5103
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Beschreibung
Zusammenfassung:Haemorrhagic kidney syndrome (HKS), a serious disease affecting Atlantic salmon on the east coast of Canada, was determined to be caused by infectious salmon anaemia virus (ISAV) through the isolation of the pathogen on the SHK-1 (salmon head kidney) cell line and confirmation by ISAV-specific immunofluorescent antibody test (IFAT) and reverse transcriptase polymerase chain reaction (RT-PCR). In addition, the defining histopathology of HKS could be reproduced following the injection of material that rendered challenged fish ISAV-positive by cell culture in the absence of any other detectable pathogen. Preliminary nucleotide sequence comparison does not suggest any direct epidemiological connection between the Canadian and Norwegian isolates.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0177-5103
DOI:10.3354/dao035145