Degradation of zearalenone and aflatoxin B1 by Lac2 from Pleurotus pulmonarius in the presence of mediators

The contamination of foods and feeds with mycotoxins has been an issue of global significance. For mycotoxin detoxification, enzymatic biodegradation using laccase has received much attention. In this study, a laccase gene lac2 from the fungus Pleurotus pulmonarius was expressed in the Pichia pastor...

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Vydané v:Toxicon (Oxford) Ročník 201; s. 1 - 8
Hlavní autori: Song, Yanyi, Wang, Yanan, Guo, Yongpeng, Qiao, Yingying, Ma, Qiugang, Ji, Cheng, Zhao, Lihong
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Elsevier Ltd 15.10.2021
Predmet:
Aflatoxin B1
biodegradation
Degradation
genes
Komagataella pastoris
Lac2 laccase
laccase
Laccase-mediator system
oxidation
Pleurotus pulmonarius
temperature
yeasts
Zearalenone
Degradation
Aflatoxin B1
Zearalenone
Laccase-mediator system
Pleurotus pulmonarius
Lac2 laccase
ISSN:0041-0101, 1879-3150, 1879-3150
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Shrnutí:The contamination of foods and feeds with mycotoxins has been an issue of global significance. For mycotoxin detoxification, enzymatic biodegradation using laccase has received much attention. In this study, a laccase gene lac2 from the fungus Pleurotus pulmonarius was expressed in the Pichia pastoris X33 yeast strain to produce recombinant proteins. Enzymatic properties of recombinant Lac2 and its ability to degrade zearalenone (ZEN) and Aflatoxin B1 (AFB1) in the presence of four mediators (ABTS, TEMPO, AS and SA) were investigated. Result showed that the optimum pH and temperature of recombinant Lac2 were 3.5 and 55 °C, respectively. Lac2 was not sensitive to heat and stable under both acidic and alkaline conditions. Lac2-ABTS and Lac2-AS were efficient systems for ZEN degradation over a wide range of pH (4–8) and temperature (40–60 °C). Lac2-AS was the most efficient system for AFB1 degradation, reaching 99.82% of degradation at pH 7 and 37 °C after 1 h of incubation. Finally, the Lac2-mediator oxidation products were structurally characterized. This study lays a solid foundation for the application of Lac2 laccase combined with AS for degrading mycotoxin in food and feed. •Our study provides useful information for the Pleurotus pulmonarius laccase2.•The use of mediators enhances the mycotoxins degradation ability of laccase2.•The laccase-mediator oxidation products were structurally characterized.•The laccase-mediator reaction mechanisms were discussed.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0041-0101
1879-3150
1879-3150
DOI:10.1016/j.toxicon.2021.08.003