Molecular genetic characteristic of dinucleotide microsatellite loci in parthenogenetic lizards Darevskia unisexualis

In the present study, the first molecular genetic investigation of dinucleotide (GT) n microsatellite loci in parthenogenetic lizards Darevskia unisexualis was performed. New polymorphic locus, Du214, (GenBank Ac. No. EU252542) was identified and characterized in detail. It was demonstrated that all...

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Bibliographic Details
Published in:Russian journal of genetics Vol. 45; no. 2; pp. 203 - 210
Main Authors: Omelchenko, A. V, Korchagin, V. I, Sevast'yanova, G. A, Ryskov, A. P, Tokarskaya, O. N
Format: Journal Article
Language:English
Published: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01.02.2009
SP MAIK Nauka/Interperiodica
Subjects:
alleles
Animal Genetics
Animal Genetics and Genomics
Biomedical and Life Sciences
Biomedicine
gene frequency
gene pool
genetic variation
Human Genetics
Lacertilia
lizards
loci
Microbial Genetics and Genomics
microsatellite repeats
parthenogenesis
point mutation
Microsatellite Cluster
Microsatellite Locus
Molecular Genetic Characteristic
Allelic Variant
Bisexual Species
ISSN:1022-7954, 1608-3369
Online Access:Get full text
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Summary:In the present study, the first molecular genetic investigation of dinucleotide (GT) n microsatellite loci in parthenogenetic lizards Darevskia unisexualis was performed. New polymorphic locus, Du214, (GenBank Ac. No. EU252542) was identified and characterized in detail. It was demonstrated that allele of this locus differed in the size and structure of microsatellite locus, as well as in point mutations, the combinations of which enabled the isolation of stabile fixed double nucleotide substitutions A-A (alleles 2 and 4) and G-T (alleles 1, 3, 5, and 6). Double nucleotide substitutions described were also identified in the orthlogous loci of the parental species genomes, D. raddei (G-T) and D. valentine (A-A). Based on the analysis of allele distribution pattern at this locus in all populations of parthenospecies D. unisexualis, mathematic model was elaborated and realized. Using this model, frequencies of allelic variants for all populations of the species of interest were calculated and population genetic structure of D. unisexualis was characterized. Genetic contribution of each population to the species gene pool was determined. The data obtained demonstrated that microsatellite variation was one of the factors of clonal and genetic diversity of a parthenospecies.
Bibliography:http://dx.doi.org/10.1134/S1022795409020112
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ISSN:1022-7954
1608-3369
DOI:10.1134/S1022795409020112