Bio-orthogonal tuning of matrix properties during 3D cell culture to induce morphological and phenotypic changes

Described herein is a protocol for producing a synthetic extracellular matrix that can be modified in situ during three-dimensional cell culture. The hydrogel platform is established using modular building blocks employing bio-orthogonal tetrazine (Tz) ligation with slow (norbornene, Nb) and fast (t...

Full description

Bibliographic Details

Published in:	Nature protocols Vol. 20; no. 3; p. 727
Main Authors:	Gao, Hanyuan, Pol, Mugdha, Makara, Colette A, Song, Jiyeon, Zhang, He, Zou, Xiaoyu, Benson, Jamie M, Burris, David L, Fox, Joseph M, Jia, Xinqiao
Format:	Journal Article
Language:	English
Published:	England 01.03.2025
Subjects:	Animals Cell Culture Techniques - methods Cell Culture Techniques, Three Dimensional - methods Cyclooctanes - chemistry Extracellular Matrix - chemistry Extracellular Matrix - metabolism Humans Hydrogels - chemistry Mesenchymal Stem Cells - cytology Norbornanes - chemistry Phenotype
ISSN:	1750-2799, 1750-2799
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!