Bio-orthogonal tuning of matrix properties during 3D cell culture to induce morphological and phenotypic changes

Described herein is a protocol for producing a synthetic extracellular matrix that can be modified in situ during three-dimensional cell culture. The hydrogel platform is established using modular building blocks employing bio-orthogonal tetrazine (Tz) ligation with slow (norbornene, Nb) and fast (t...

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Veröffentlicht in:	Nature protocols Jg. 20; H. 3; S. 727
Hauptverfasser:	Gao, Hanyuan, Pol, Mugdha, Makara, Colette A, Song, Jiyeon, Zhang, He, Zou, Xiaoyu, Benson, Jamie M, Burris, David L, Fox, Joseph M, Jia, Xinqiao
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	England 01.03.2025
Schlagworte:	Animals Cell Culture Techniques - methods Cell Culture Techniques, Three Dimensional - methods Cyclooctanes - chemistry Extracellular Matrix - chemistry Extracellular Matrix - metabolism Humans Hydrogels - chemistry Mesenchymal Stem Cells - cytology Norbornanes - chemistry Phenotype
ISSN:	1750-2799, 1750-2799
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