Functionalization of bacterial microcompartment shell interior with cysteine containing peptides enhances the iron and cobalt loading capacity

Bacterial microcompartments (BMCs) are prokaryotic organelles involved in several biochemical processes in bacterial cells. These cellular substructures consist of an icosahedral shell and an encapsulated enzymatic core. The outer shells of BMCs have been proposed as an attractive platform for the c...

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Bibliographic Details
Published in:Biometals Vol. 37; no. 1; pp. 267 - 274
Main Authors: Kalnins, Gints, Bertins, Maris, Viksna, Arturs, Tars, Kaspars
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01.02.2024
Springer Nature B.V
Subjects:
Bacteria
Bacterial Proteins - chemistry
Biochemistry
Biomedical and Life Sciences
Brief Report
Cell Biology
Chromatography
Cloning
Cobalt
Cysteine
Enzymes
Gas flow
Iron
Life Sciences
Lumens
Medicine/Public Health
Microbiology
Nanomaterials
Nanotechnology
Nickel
Organelles
Peptides
Pharmacology/Toxicology
Plant Physiology
Proteins
Shells
Zinc
Bacterial microcompartments
Protein shells
Metal-binding peptides
ISSN:0966-0844, 1572-8773, 1572-8773
Online Access:Get full text
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Summary:Bacterial microcompartments (BMCs) are prokaryotic organelles involved in several biochemical processes in bacterial cells. These cellular substructures consist of an icosahedral shell and an encapsulated enzymatic core. The outer shells of BMCs have been proposed as an attractive platform for the creation of novel nanomaterials, nanocages, and nanoreactors. In this study, we present a method for functionalizing recombinant GRM2-type BMC shell lumens with short cysteine-containing sequences and demonstrate that the iron and cobalt loading capacity of such modified shells is markedly increased. These results also imply that a passive flow of cobalt and iron atoms across the BMC shell could be possible.
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ISSN:0966-0844
1572-8773
1572-8773
DOI:10.1007/s10534-023-00538-1