Non-heart-beating versus cadaveric and living-donor livers: differences in inflammatory markers before transplantation

Liver transplantation from non-heart-beating donors (NHBD) has been reintroduced into clinical practice to increase the donor pool; however, little is known about the immune status of NHBD livers. The aim of this study was to assess intragraft cell populations and inflammatory markers in NHBD and to...

Full description

Saved in:
Bibliographic Details
Published in:Transplantation Vol. 75; no. 8; p. 1386
Main Authors: Jassem, Wayel, Koo, Dicken D H, Muiesan, Paolo, Cerundolo, Lucia, Rela, Mohamed, Fuggle, Susan V, Heaton, Nigel D
Format: Journal Article
Language:English
Published: United States 27.04.2003
Subjects:
Adult
Antigens, CD - metabolism
Antigens, Differentiation, Myelomonocytic - metabolism
Biomarkers - analysis
Cadaver
Critical Care
Female
Heart Arrest
Humans
Immunohistochemistry
Inflammation - metabolism
Inflammation Mediators - metabolism
Intercellular Adhesion Molecule-1 - metabolism
Liver - metabolism
Liver Transplantation
Living Donors
Male
Middle Aged
Tissue Donors
ISSN:0041-1337
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract Liver transplantation from non-heart-beating donors (NHBD) has been reintroduced into clinical practice to increase the donor pool; however, little is known about the immune status of NHBD livers. The aim of this study was to assess intragraft cell populations and inflammatory markers in NHBD and to compare the findings with cadaveric and living-related donor (LRD) livers. Biopsy specimens were obtained from controlled NHBD (n=9), conventional cadaveric (n=22), and living-donor (n=10) livers at the end of cold storage. Cryostat sections were stained for monocytes-macrophages, T lymphocytes, and intercellular adhesion molecule (ICAM)-1. The levels of leukocyte infiltration in NHBD reflected those found in conventional cadaver donors and were significantly higher than in LRD livers. Similar levels of CD68+ monocytes-macrophages were detected in cadaver (4.0+/-1.2%) and NHBD livers (4.6+/-1.2%) and were significantly greater than in the LRD livers (2.6+/-0.5%, P<0.01). Furthermore, the levels of T lymphocytes in NHBD (1.1+/-0.6%) and cadaver donors (1.5+/-0.8%) were similar, and were higher than in LRD (vs. 0.47+/-0.3%, P<0.05). Twelve of 22 (60%) cadaver livers had high levels of ICAM-1 expression (grade 3), compared with only 1 of 10 (10%) LRD livers (P=0.02). Four of nine (44%) controlled NHBD livers expressed high levels of ICAM-1. The results demonstrate that livers obtained from controlled NHBD before transplantation are similar to conventional cadaver donors regarding the level of leukocyte infiltration. Nevertheless, lower levels of ICAM-1 were detected in NHBD, suggesting less exposure to inflammatory mediators than conventional cadaver donor livers.
AbstractList Liver transplantation from non-heart-beating donors (NHBD) has been reintroduced into clinical practice to increase the donor pool; however, little is known about the immune status of NHBD livers. The aim of this study was to assess intragraft cell populations and inflammatory markers in NHBD and to compare the findings with cadaveric and living-related donor (LRD) livers.BACKGROUNDLiver transplantation from non-heart-beating donors (NHBD) has been reintroduced into clinical practice to increase the donor pool; however, little is known about the immune status of NHBD livers. The aim of this study was to assess intragraft cell populations and inflammatory markers in NHBD and to compare the findings with cadaveric and living-related donor (LRD) livers.Biopsy specimens were obtained from controlled NHBD (n=9), conventional cadaveric (n=22), and living-donor (n=10) livers at the end of cold storage. Cryostat sections were stained for monocytes-macrophages, T lymphocytes, and intercellular adhesion molecule (ICAM)-1.METHODSBiopsy specimens were obtained from controlled NHBD (n=9), conventional cadaveric (n=22), and living-donor (n=10) livers at the end of cold storage. Cryostat sections were stained for monocytes-macrophages, T lymphocytes, and intercellular adhesion molecule (ICAM)-1.The levels of leukocyte infiltration in NHBD reflected those found in conventional cadaver donors and were significantly higher than in LRD livers. Similar levels of CD68+ monocytes-macrophages were detected in cadaver (4.0+/-1.2%) and NHBD livers (4.6+/-1.2%) and were significantly greater than in the LRD livers (2.6+/-0.5%, P<0.01). Furthermore, the levels of T lymphocytes in NHBD (1.1+/-0.6%) and cadaver donors (1.5+/-0.8%) were similar, and were higher than in LRD (vs. 0.47+/-0.3%, P<0.05). Twelve of 22 (60%) cadaver livers had high levels of ICAM-1 expression (grade 3), compared with only 1 of 10 (10%) LRD livers (P=0.02). Four of nine (44%) controlled NHBD livers expressed high levels of ICAM-1.RESULTSThe levels of leukocyte infiltration in NHBD reflected those found in conventional cadaver donors and were significantly higher than in LRD livers. Similar levels of CD68+ monocytes-macrophages were detected in cadaver (4.0+/-1.2%) and NHBD livers (4.6+/-1.2%) and were significantly greater than in the LRD livers (2.6+/-0.5%, P<0.01). Furthermore, the levels of T lymphocytes in NHBD (1.1+/-0.6%) and cadaver donors (1.5+/-0.8%) were similar, and were higher than in LRD (vs. 0.47+/-0.3%, P<0.05). Twelve of 22 (60%) cadaver livers had high levels of ICAM-1 expression (grade 3), compared with only 1 of 10 (10%) LRD livers (P=0.02). Four of nine (44%) controlled NHBD livers expressed high levels of ICAM-1.The results demonstrate that livers obtained from controlled NHBD before transplantation are similar to conventional cadaver donors regarding the level of leukocyte infiltration. Nevertheless, lower levels of ICAM-1 were detected in NHBD, suggesting less exposure to inflammatory mediators than conventional cadaver donor livers.CONCLUSIONSThe results demonstrate that livers obtained from controlled NHBD before transplantation are similar to conventional cadaver donors regarding the level of leukocyte infiltration. Nevertheless, lower levels of ICAM-1 were detected in NHBD, suggesting less exposure to inflammatory mediators than conventional cadaver donor livers.
Liver transplantation from non-heart-beating donors (NHBD) has been reintroduced into clinical practice to increase the donor pool; however, little is known about the immune status of NHBD livers. The aim of this study was to assess intragraft cell populations and inflammatory markers in NHBD and to compare the findings with cadaveric and living-related donor (LRD) livers. Biopsy specimens were obtained from controlled NHBD (n=9), conventional cadaveric (n=22), and living-donor (n=10) livers at the end of cold storage. Cryostat sections were stained for monocytes-macrophages, T lymphocytes, and intercellular adhesion molecule (ICAM)-1. The levels of leukocyte infiltration in NHBD reflected those found in conventional cadaver donors and were significantly higher than in LRD livers. Similar levels of CD68+ monocytes-macrophages were detected in cadaver (4.0+/-1.2%) and NHBD livers (4.6+/-1.2%) and were significantly greater than in the LRD livers (2.6+/-0.5%, P<0.01). Furthermore, the levels of T lymphocytes in NHBD (1.1+/-0.6%) and cadaver donors (1.5+/-0.8%) were similar, and were higher than in LRD (vs. 0.47+/-0.3%, P<0.05). Twelve of 22 (60%) cadaver livers had high levels of ICAM-1 expression (grade 3), compared with only 1 of 10 (10%) LRD livers (P=0.02). Four of nine (44%) controlled NHBD livers expressed high levels of ICAM-1. The results demonstrate that livers obtained from controlled NHBD before transplantation are similar to conventional cadaver donors regarding the level of leukocyte infiltration. Nevertheless, lower levels of ICAM-1 were detected in NHBD, suggesting less exposure to inflammatory mediators than conventional cadaver donor livers.
Author Fuggle, Susan V
Rela, Mohamed
Koo, Dicken D H
Jassem, Wayel
Cerundolo, Lucia
Heaton, Nigel D
Muiesan, Paolo
Author_xml – sequence: 1
  givenname: Wayel
  surname: Jassem
  fullname: Jassem, Wayel
  organization: Liver Transplant Unit, Institute of Liver Studies, King's College Hospital, Denmark Hill, London, United Kingdom
– sequence: 2
  givenname: Dicken D H
  surname: Koo
  fullname: Koo, Dicken D H
– sequence: 3
  givenname: Paolo
  surname: Muiesan
  fullname: Muiesan, Paolo
– sequence: 4
  givenname: Lucia
  surname: Cerundolo
  fullname: Cerundolo, Lucia
– sequence: 5
  givenname: Mohamed
  surname: Rela
  fullname: Rela, Mohamed
– sequence: 6
  givenname: Susan V
  surname: Fuggle
  fullname: Fuggle, Susan V
– sequence: 7
  givenname: Nigel D
  surname: Heaton
  fullname: Heaton, Nigel D
BackLink https://www.ncbi.nlm.nih.gov/pubmed/12717235$$D View this record in MEDLINE/PubMed
BookMark eNo1kEtPwzAMx3MYYmPwFVC4cGvJo21SbmjiJU3AYZyrNHGg0CYjaSft25OJYVmyLf_09-MMzZx3gNAVJTkltbghNN-85eRgFZMVz0klJMtlPUMLQgqaUc7FHJ3F-JWQkgtxiuaUCSoYLxdo9-Jd9gkqjFkLauzcB95BiFPEWhmV0k5j5Qzuu13qZcY7Hw5FYm6x6ayFAE5DxJ1Lbns1DGr0YY8HFb4ThFuwPgAeg3Jx2ys3piHenaMTq_oIF8e4RO8P95vVU7Z-fXxe3a0zzUUxZrWQtGbWaA1VBUwTK21Vt1C0shVGGykIYayoiAFVAWW6LI00pW1lSTnRli3R9Z_uNvifCeLYDF3U0KdFwE-xEZxxQqlM4OURnNoBTLMNXbpg3_x_iv0CWRtv6g
CitedBy_id crossref_primary_10_1016_j_trre_2015_11_001
crossref_primary_10_1016_S0140_6736_05_66382_9
crossref_primary_10_1016_j_jss_2014_02_008
crossref_primary_10_1016_j_cursur_2006_08_001
crossref_primary_10_1016_j_jss_2015_04_057
crossref_primary_10_1053_j_semtcvs_2004_09_014
crossref_primary_10_1016_j_transproceed_2015_10_081
crossref_primary_10_1016_j_bbi_2009_04_006
crossref_primary_10_1038_srep17737
crossref_primary_10_1111_j_1600_6143_2007_01799_x
crossref_primary_10_1089_scd_2014_0485
crossref_primary_10_1016_j_transproceed_2008_10_004
crossref_primary_10_1002_lt_20732
crossref_primary_10_1002_lt_25608
crossref_primary_10_1016_j_transproceed_2021_07_055
crossref_primary_10_1111_j_1600_6143_2011_03466_x
crossref_primary_10_1016_j_jss_2012_06_031
crossref_primary_10_1016_j_transproceed_2007_01_071
crossref_primary_10_1371_journal_pone_0139791
crossref_primary_10_1097_TP_0b013e31828ee151
crossref_primary_10_1016_j_athoracsur_2005_09_034
crossref_primary_10_1177_152692480401400205
crossref_primary_10_1016_S1594_5804_09_60031_0
crossref_primary_10_1067_j_cpsurg_2004_12_003
crossref_primary_10_1164_rccm_200501_098WS
crossref_primary_10_7182_prtr_14_2_cx44767r26575316
crossref_primary_10_1097_01_tp_0000225829_07208_58
crossref_primary_10_1111_j_1600_6143_2007_01937_x
crossref_primary_10_1016_j_transproceed_2009_05_039
crossref_primary_10_1007_s00011_012_0550_7
ContentType Journal Article
DBID CGR
CUY
CVF
ECM
EIF
NPM
7X8
DOI 10.1097/01.TP.0000062863.06782.89
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList MEDLINE - Academic
MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: 7X8
  name: MEDLINE - Academic
  url: https://search.proquest.com/medline
  sourceTypes: Aggregation Database
DeliveryMethod no_fulltext_linktorsrc
Discipline Medicine
Anatomy & Physiology
ExternalDocumentID 12717235
Genre Journal Article
Comparative Study
GroupedDBID ---
-~X
.-D
.55
.GJ
.XZ
.Z2
01R
0R~
123
1J1
3O-
40H
4Q1
4Q2
4Q3
53G
5RE
5VS
77Y
7O~
AAAAV
AAAXR
AAGIX
AAHPQ
AAIQE
AAJCS
AAMOA
AAMTA
AARTV
AASOK
AAUEB
AAXQO
AAYEP
ABBUW
ABDIG
ABJNI
ABOCM
ABPPZ
ABXVJ
ABZAD
ACCJW
ACDDN
ACEWG
ACGFO
ACGFS
ACIJW
ACILI
ACWDW
ACWRI
ACXNZ
ADFPA
ADGGA
ADHPY
ADNKB
AE3
AE6
AEETU
AENEX
AFDTB
AFEXH
AFFNX
AFUWQ
AGINI
AHOMT
AHQNM
AHVBC
AIJEX
AINUH
AJCLO
AJIOK
AJNWD
AJNYG
AJZMW
ALKUP
ALMA_UNASSIGNED_HOLDINGS
AMJPA
AMNEI
AWKKM
BOYCO
BQLVK
BS7
C45
CGR
CS3
CUY
CVF
DIWNM
DU5
DUNZO
E.X
EBS
ECM
EIF
EJD
EX3
F2K
F2L
F2M
F2N
F5P
FCALG
FL-
FW0
H0~
HZ~
IH2
IKREB
IKYAY
IN~
J5H
JF9
JG8
JK3
JK8
K8S
KD2
KMI
L-C
L7B
N9A
NPM
N~7
N~B
N~M
O9-
OAG
OAH
OCUKA
ODA
ODMTH
OHYEH
OJAPA
OL1
OLG
OLH
OLU
OLV
OLW
OLY
OLZ
OPUJH
ORVUJ
OUVQU
OVD
OVDNE
OVIDH
OVLEI
OVOZU
OWU
OWV
OWW
OWX
OWY
OWZ
OXXIT
P2P
R58
RLZ
S4R
S4S
T8P
TEORI
V2I
VVN
W3M
WOQ
WOW
X3V
X3W
X7M
XXN
XYM
YFH
YOC
ZFV
ZGI
ZZMQN
7X8
ABPXF
ACDOF
ACZKN
ADKSD
AFNMH
ID FETCH-LOGICAL-c374t-978192fdcce66e2c0f8f69be4b8b7dcd870022460dea6e12c55d8d5fb85130cf2
IEDL.DBID 7X8
ISICitedReferencesCount 28
ISICitedReferencesURI http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000182573100064&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN 0041-1337
IngestDate Sun Sep 28 01:12:15 EDT 2025
Wed Feb 19 01:44:37 EST 2025
IsPeerReviewed true
IsScholarly true
Issue 8
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c374t-978192fdcce66e2c0f8f69be4b8b7dcd870022460dea6e12c55d8d5fb85130cf2
Notes ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
PMID 12717235
PQID 73230118
PQPubID 23479
ParticipantIDs proquest_miscellaneous_73230118
pubmed_primary_12717235
PublicationCentury 2000
PublicationDate 2003-04-27
PublicationDateYYYYMMDD 2003-04-27
PublicationDate_xml – month: 04
  year: 2003
  text: 2003-04-27
  day: 27
PublicationDecade 2000
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Transplantation
PublicationTitleAlternate Transplantation
PublicationYear 2003
SSID ssj0005377
Score 1.8817959
Snippet Liver transplantation from non-heart-beating donors (NHBD) has been reintroduced into clinical practice to increase the donor pool; however, little is known...
SourceID proquest
pubmed
SourceType Aggregation Database
Index Database
StartPage 1386
SubjectTerms Adult
Antigens, CD - metabolism
Antigens, Differentiation, Myelomonocytic - metabolism
Biomarkers - analysis
Cadaver
Critical Care
Female
Heart Arrest
Humans
Immunohistochemistry
Inflammation - metabolism
Inflammation Mediators - metabolism
Intercellular Adhesion Molecule-1 - metabolism
Liver - metabolism
Liver Transplantation
Living Donors
Male
Middle Aged
Tissue Donors
Title Non-heart-beating versus cadaveric and living-donor livers: differences in inflammatory markers before transplantation
URI https://www.ncbi.nlm.nih.gov/pubmed/12717235
https://www.proquest.com/docview/73230118
Volume 75
WOSCitedRecordID wos000182573100064&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1La9wwEB7SJoRe-kj6SJ8KhN601Uq2ZZdCCaWhlyx7SGFvizySykJWTnc3C_33nZHXza09FIzBYIORPo-_eX0DcNY4x6wdJYEjyKLwSrbeVbJRSOwfrXXK52ETdjKpZ7Nmugefhl4YLqscbGI21L5DjpF_sEYzFuvPNz8lz4zi3OpugMY92DdEZBjTdnanFV4a2ytmFmNJnpg9hNM_ko3j0dW0ly7k1kwzYputR_VfeGb-31w8-r83fQwPdzxTnPfAeAJ7IR3B8XkiH3v5S7wXufIzh9SP4PByl2A_hu2kS5KHXG9ky2wy_RBct3G7Fui823J2R7jkxfWCAxHSd6lb8QXd81EMw1bI9IhFoiMS3JY5jS-WXAa0Wos2EEsOYpM11a9d3_iUnsL3i69XX77J3WgGicYWG8lKWY2OHjFUVdCoYh2rpg1FW7fWoycrkKXqlA-uCmONZelrX8aWCJ5RGPUzuJ-6FF6AqEI0UZtQtUUonNauUq4kq6ARUaEPJ_BuWOY5QZ_zGS6F7nY9Hxb6BJ73OzW_6RU65mNNXqo25ct_PvsKHuTyPFVIbV_DfqSPPryBA9xuFuvV24woOk-ml78B6E7XlQ
linkProvider ProQuest
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Non-heart-beating+versus+cadaveric+and+living-donor+livers%3A+differences+in+inflammatory+markers+before+transplantation&rft.jtitle=Transplantation&rft.au=Jassem%2C+Wayel&rft.au=Koo%2C+Dicken+D+H&rft.au=Muiesan%2C+Paolo&rft.au=Cerundolo%2C+Lucia&rft.date=2003-04-27&rft.issn=0041-1337&rft.volume=75&rft.issue=8&rft.spage=1386&rft_id=info:doi/10.1097%2F01.TP.0000062863.06782.89&rft_id=info%3Apmid%2F12717235&rft_id=info%3Apmid%2F12717235&rft.externalDocID=12717235
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0041-1337&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0041-1337&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0041-1337&client=summon