Discovery of an Ultra‐rapid and Sensitive Lysosomal Fluorescence Lipophagy Process

Non‐invasive dynamic tracking of lysosomes and their interactions with other organelles is important for the study of lysosomal function and related diseases. However, many fluorescent dyes developed so far to target lysosomes cannot be used to monitor these processes due to the high concentrations...

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Vydáno v:Angewandte Chemie International Edition Ročník 61; číslo 11; s. e202116439 - n/a
Hlavní autoři: Zhang, Hong, Shi, Lei, Li, Kun, Liu, Xin, Won, Miae, Liu, Yan‐Zhao, Choe, Youmi, Liu, Xin‐Yao, Liu, Yan‐Hong, Chen, Shan‐Yong, Yu, Kang‐Kang, Kim, Jong Seung, Yu, Xiao‐Qi
Médium: Journal Article
Jazyk:angličtina
Vydáno: WEINHEIM Wiley 07.03.2022
Wiley Subscription Services, Inc
Vydání:International ed. in English
Témata:
Autophagy
Chemistry
Chemistry, Multidisciplinary
Dyes
Fluorescence
Fluorescent dyes
Fluorescent indicators
Large Stokes Shift
Lipophagy
Low concentrations
Lysosomes
Near-Infrared
Organelles
Physical Sciences
Probes
Science & Technology
CELLS
ADIPOCYTES
DYES
Lipophagy
Large Stokes Shift
Lysosomes
AUTOPHAGY
DIFFERENTIATION
MODEL
Near-Infrared
PROBES
ISSN:1433-7851, 1521-3773, 1521-3773
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Shrnutí:Non‐invasive dynamic tracking of lysosomes and their interactions with other organelles is important for the study of lysosomal function and related diseases. However, many fluorescent dyes developed so far to target lysosomes cannot be used to monitor these processes due to the high concentrations required for imaging, long cell penetration times, and non‐ideal photostability. In this regard, we synthesized three lysosomal targeting probes with large Stokes shifts, good stability, and high brightness. The Q‐P‐ARh dye, developed by us for the first time, can stain lysosomes at ultra‐low concentrations (1.0 nM) without affecting the physiological functions of the lysosomes. More importantly, its excellent anti‐interference ability and ultrafast lysosomal staining ability (within 1.0 min) clearly monitored the entire dynamic process of lipophagy. Ultimately, this method can greatly contribute to the study of autophagy pathways. This novel fluorescence platform shows great promise for the development of biological probes for application in pathological environments. A series of brand‐new large Stokes shift and highly stable fluorescent dyes were constructed. In particular, the Q‐P‐ARh fluorescent dye as a near‐infrared emission lysosomal‐specific probe with ultra‐low concentration and ultra‐fast staining characteristics for the complete lipophagy process imaging is presented.
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ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.202116439