Penetration depth of sodium hypochlorite into dentinal tubules influenced by different agitation systems

Objectives: To compare the effect of different agitation systems on the penetration depth of sodium hypochlorite into dentinal tubules: An in vitro study. Background: Studies have found the presence of bacteria in dentinal tubule up to 200 μ. Passive irrigation using NaOCl may not adequately elimina...

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Published in:Endodontology : journal of Indian Endodontic Society Vol. 33; no. 2; pp. 81 - 85
Main Authors: Karad, Keyur, Kolhe, Swapnil, Gulve, Meenal, Aher, Gayatri, Kolhe, Priyanka
Format: Journal Article
Language:English
Published: Wolters Kluwer - Medknow Publications 01.04.2021
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ISSN:0970-7212
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Abstract Objectives: To compare the effect of different agitation systems on the penetration depth of sodium hypochlorite into dentinal tubules: An in vitro study. Background: Studies have found the presence of bacteria in dentinal tubule up to 200 μ. Passive irrigation using NaOCl may not adequately eliminate these bacteria. Hence, it is mandatory to use agitation systems during irrigation and disinfection of root canal. Materials and Method: Eighty extracted teeth with single canals were used. Standardized access cavity preparation and working length determination were done using visual method. All canals were instrumented till #30, 6%. All samples were immersed in crystal violet dye. The samples were randomly divided into four groups depending on the type of agitation system used (n = 20): Group A: manual dynamic agitation (MDA), Group B: EndoActivator (EA), Group C: EndoUltra (EU), and Group D: EndoVac (EV). Each agitation system was used with 5 ml of 5.25% NaOCl, and each tooth was sectioned into two halves. These sections were analyzed under a stereomicroscope, and NaOCl penetration depth was measured at coronal, middle, and apical third levels. Statistical Analysis Used: Statistical analysis was performed using one-way ANOVA and Tukey's post hoc test. Results: At all levels of examination, EU significantly showed a maximum penetration depth of NaOCl throughout the length of root canal, followed by EA, MDA, and EV. Conclusion: Ultrasonic agitation using EU is the most effective method to disinfect the dentinal tubules throughout the length of root canal than EA and MDA. EV showed the least penetration depth of sodium hypochlorite.
AbstractList Objectives: To compare the effect of different agitation systems on the penetration depth of sodium hypochlorite into dentinal tubules: An in vitro study. Background: Studies have found the presence of bacteria in dentinal tubule up to 200 μ. Passive irrigation using NaOCl may not adequately eliminate these bacteria. Hence, it is mandatory to use agitation systems during irrigation and disinfection of root canal. Materials and Method: Eighty extracted teeth with single canals were used. Standardized access cavity preparation and working length determination were done using visual method. All canals were instrumented till #30, 6%. All samples were immersed in crystal violet dye. The samples were randomly divided into four groups depending on the type of agitation system used (n = 20): Group A: manual dynamic agitation (MDA), Group B: EndoActivator (EA), Group C: EndoUltra (EU), and Group D: EndoVac (EV). Each agitation system was used with 5 ml of 5.25% NaOCl, and each tooth was sectioned into two halves. These sections were analyzed under a stereomicroscope, and NaOCl penetration depth was measured at coronal, middle, and apical third levels. Statistical Analysis Used: Statistical analysis was performed using one-way ANOVA and Tukey's post hoc test. Results: At all levels of examination, EU significantly showed a maximum penetration depth of NaOCl throughout the length of root canal, followed by EA, MDA, and EV. Conclusion: Ultrasonic agitation using EU is the most effective method to disinfect the dentinal tubules throughout the length of root canal than EA and MDA. EV showed the least penetration depth of sodium hypochlorite.
Objectives: To compare the effect of different agitation systems on the penetration depth of sodium hypochlorite into dentinal tubules: An in vitro study. Background: Studies have found the presence of bacteria in dentinal tubule up to 200 μ. Passive irrigation using NaOCl may not adequately eliminate these bacteria. Hence, it is mandatory to use agitation systems during irrigation and disinfection of root canal. Materials and Method: Eighty extracted teeth with single canals were used. Standardized access cavity preparation and working length determination were done using visual method. All canals were instrumented till #30, 6%. All samples were immersed in crystal violet dye. The samples were randomly divided into four groups depending on the type of agitation system used (n = 20): Group A: manual dynamic agitation (MDA), Group B: EndoActivator (EA), Group C: EndoUltra (EU), and Group D: EndoVac (EV). Each agitation system was used with 5 ml of 5.25% NaOCl, and each tooth was sectioned into two halves. These sections were analyzed under a stereomicroscope, and NaOCl penetration depth was measured at coronal, middle, and apical third levels. Statistical Analysis Used: Statistical analysis was performed using one-way ANOVA and Tukey's post hoc test. Results: At all levels of examination, EU significantly showed a maximum penetration depth of NaOCl throughout the length of root canal, followed by EA, MDA, and EV. Conclusion: Ultrasonic agitation using EU is the most effective method to disinfect the dentinal tubules throughout the length of root canal than EA and MDA. EV showed the least penetration depth of sodium hypochlorite.
Audience Academic
Author Karad, Keyur
Kolhe, Swapnil
Kolhe, Priyanka
Gulve, Meenal
Aher, Gayatri
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Cites_doi 10.4103/JCD.JCD_387_18
10.1016/S0099-2399(06)80508-X
10.1007/s00784-016-2013-y
10.1016/S0099-2399(75)80097-5
10.1046/j.1365-2591.2003.00646.x
10.4103/JCD.JCD_286_16
10.1016/S0099-2399(06)81284-7
10.4103/JCD.JCD_290_16
10.17796/1053-4625-42.5.6
10.1177/00220345700490063601
10.1016/S0099-2399(83)80162-9
10.1590/0103-6440201600726
10.1016/S0099-2399(82)80073-3
10.1111/j.1365-2591.1993.tb00766.x
10.1111/j.1365-2591.1989.tb00501.x
10.17126/joralres.2017.089
10.4103/JCD.JCD_328_17
10.4103/JCD.JCD_390_19
10.1016/j.joen.2009.03.010
10.31080/ASDS.2020.04.0983
10.4103/1658-5984.155451
10.1016/j.joen.2010.02.005
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sodium hypochlorite
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References Ciucchi (R16-5-20240829) 1989; 22
Foster (R13-5-20240829) 1993; 19
Eick (R6-5-20240829) 1970; 49
Cesario (R1-5-20240829) 2018; 21
Baker (R11-5-20240829) 1975; 1
Chow (R10-5-20240829) 1983; 9
Akcay (R19-5-20240829) 2017; 21
Souza (R3-5-20240829) 2019; 22
Nangia (R2-5-20240829) 2020; 23
Plotino (R20-5-20240829) 2016; 27
Macías (R21-5-20240829) 2018; 7
Venumbaka (R25-5-20240829) 2018; 42
Wu (R5-5-20240829) 2003; 36
Lussi (R7-5-20240829) 1993; 19
Zou (R18-5-20240829) 2010; 36
Goldman (R9-5-20240829) 1982; 8
Chatterjee (R23-5-20240829) 2020; 5
Cheung (R12-5-20240829) 1993; 26
Agrawal (R24-5-20240829) 2020; 4
Gu (R17-5-20240829) 2009; 35
Kumar (R4-5-20240829) 2018; 21
Jaiswal (R8-5-20240829) 2018; 21
Nair (R15-5-20240829) 2011; 5
Mohammadi (R22-5-20240829) 2015; 10
References_xml – volume: 22
  start-page: 155
  year: 2019
  ident: R3-5-20240829
  article-title: Efficacy of passive ultrasonic irrigation, continuous ultrasonic irrigation versus irrigation with reciprocating activation device in penetration into main and simulated lateral canals
  publication-title: J Conserv Dent
  doi: 10.4103/JCD.JCD_387_18
– volume: 19
  start-page: 136
  year: 1993
  ident: R13-5-20240829
  article-title: Effect of smear layer removal on the diffusion of calcium hydroxide through radicular dentin
  publication-title: J Endod
  doi: 10.1016/S0099-2399(06)80508-X
– volume: 5
  start-page: 1
  year: 2011
  ident: R15-5-20240829
  article-title: Advances in root canal disinfection
  publication-title: J Pharm Biomed Sci
– volume: 21
  start-page: 2205
  year: 2017
  ident: R19-5-20240829
  article-title: Effect of photon-initiated photoacoustic streaming, passive ultrasonic, and sonic irrigation techniques on dentinal tubule penetration of irrigation solution: A confocal microscopic study
  publication-title: Clin Oral Investig
  doi: 10.1007/s00784-016-2013-y
– volume: 1
  start-page: 127
  year: 1975
  ident: R11-5-20240829
  article-title: Scanning electron microscopic study of the efficacy of various irrigating solutions
  publication-title: J Endod
  doi: 10.1016/S0099-2399(75)80097-5
– volume: 36
  start-page: 218
  year: 2003
  ident: R5-5-20240829
  article-title: The capability of two hand instrumentation techniques to remove the inner layer of dentine in oval canals
  publication-title: Int Endod J
  doi: 10.1046/j.1365-2591.2003.00646.x
– volume: 21
  start-page: 383
  year: 2018
  ident: R1-5-20240829
  article-title: Comparisons by microcomputed tomography of the efficiency of different irrigation techniques for removing dentinal debris from artificial grooves
  publication-title: J Conserv Dent
  doi: 10.4103/JCD.JCD_286_16
– volume: 19
  start-page: 549
  year: 1993
  ident: R7-5-20240829
  article-title: A novel noninstrumented technique for cleansing the root canal system
  publication-title: J Endod
  doi: 10.1016/S0099-2399(06)81284-7
– volume: 21
  start-page: 419
  year: 2018
  ident: R8-5-20240829
  article-title: Comparative analysis of smear layer removal by conventional endodontic irrigants with a newly experimented irrigant-fumaric acid: A scanning electron microscopic study
  publication-title: J Conserv Dent
  doi: 10.4103/JCD.JCD_290_16
– volume: 42
  start-page: 355
  year: 2018
  ident: R25-5-20240829
  article-title: Comparative evaluation of endovac and conventional irrigating syringe on apical extrusion in primary molars. An in vitro study
  publication-title: J Clin Pediatr Dent
  doi: 10.17796/1053-4625-42.5.6
– volume: 49
  start-page: 1359
  year: 1970
  ident: R6-5-20240829
  article-title: Scanning electron microscopy of cut tooth surfaces and identification of debris by use of the electron microprobe
  publication-title: J Dent Res
  doi: 10.1177/00220345700490063601
– volume: 9
  start-page: 475
  year: 1983
  ident: R10-5-20240829
  article-title: Mechanical effectiveness of root canal irrigation
  publication-title: J Endod
  doi: 10.1016/S0099-2399(83)80162-9
– volume: 27
  start-page: 3
  year: 2016
  ident: R20-5-20240829
  article-title: New technologies to improve root canal disinfection
  publication-title: Braz Dent J
  doi: 10.1590/0103-6440201600726
– volume: 8
  start-page: 487
  year: 1982
  ident: R9-5-20240829
  article-title: The efficacy of several endodontic irrigating solutions: A scanning electron microscopic study: Part 2
  publication-title: J Endod
  doi: 10.1016/S0099-2399(82)80073-3
– volume: 26
  start-page: 334
  year: 1993
  ident: R12-5-20240829
  article-title: In vitro cleaning ability of root canal irrigants with and without endosonics
  publication-title: Int Endod J
  doi: 10.1111/j.1365-2591.1993.tb00766.x
– volume: 22
  start-page: 21
  year: 1989
  ident: R16-5-20240829
  article-title: The effectiveness of different endodontic irrigation procedures on the removal of the smear layer: A scanning electron microscopic study
  publication-title: Int Endod J
  doi: 10.1111/j.1365-2591.1989.tb00501.x
– volume: 7
  start-page: 24
  year: 2018
  ident: R21-5-20240829
  article-title: Effect of sonic versus ultrasonic activation on aqueous solution penetration in root canal dentin
  publication-title: J Oral Res
  doi: 10.17126/joralres.2017.089
– volume: 21
  start-page: 438
  year: 2018
  ident: R4-5-20240829
  article-title: An in vitro comparison of the antimicrobial efficacy of positive pressure and negative pressure irrigation techniques in root canals infected with Enterococcus faecalis
  publication-title: J Conserv Dent
  doi: 10.4103/JCD.JCD_328_17
– volume: 10
  start-page: 216
  year: 2015
  ident: R22-5-20240829
  article-title: Impact of ultrasonic activation on the effectiveness of sodium hypochlorite: A review
  publication-title: Iran Endod J
– volume: 23
  start-page: 185
  year: 2020
  ident: R2-5-20240829
  article-title: Evaluation of apical extrusion and cone-beam computed tomography assessment of irrigant penetration in oval-shaped canals, using XP Endo Finisher and EndoActivator
  publication-title: J Conserv Dent
  doi: 10.4103/JCD.JCD_390_19
– volume: 35
  start-page: 791
  year: 2009
  ident: R17-5-20240829
  article-title: Review of contemporary irrigant agitation techniques and devices
  publication-title: J Endod
  doi: 10.1016/j.joen.2009.03.010
– volume: 4
  start-page: 57
  year: 2020
  ident: R24-5-20240829
  article-title: Ability of sodium hypochlorite to clean dentinal tubules by manual or sonic activation at varying temperature: A confocal laser scanning microscopic study
  publication-title: Acta Sci Dent Sci
  doi: 10.31080/ASDS.2020.04.0983
– volume: 5
  start-page: 125
  year: 2020
  ident: R23-5-20240829
  article-title: Effect of sonic agitation, manual dynamic agitation on removal of Enterococcus faecalis biofilm
  publication-title: Saudi Endod J
  doi: 10.4103/1658-5984.155451
– volume: 36
  start-page: 793
  year: 2010
  ident: R18-5-20240829
  article-title: Penetration of sodium hypochlorite into dentin
  publication-title: J Endod
  doi: 10.1016/j.joen.2010.02.005
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SubjectTerms endoactivator
endoultra
endovac
Ethylenediaminetetraacetic acid
Root canal therapy
Sodium hypochlorite
Title Penetration depth of sodium hypochlorite into dentinal tubules influenced by different agitation systems
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