Monitoring Real-time Temperature Dynamics of a Short RNA Hairpin Using Förster Resonance Energy Transfer and Circular Dichroism

RNA secondary structures are highly dynamic and subject to prompt changes in response to the environment. Temperature in particular has a strong impact on RNA structural conformation, and temperature-sensitive RNA hairpin structures have been exploited by multiple organisms to modify the rate of tra...

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Bibliographic Details

Published in:	Bio-protocol Vol. 11; no. 6; p. e3950
Main Authors:	Balcerowicz, Martin, Di Antonio, Marco, Chung, Betty
Format:	Journal Article
Language:	English
Published:	United States Bio-Protocol 20.03.2021 Bio-protocol LLC
Subjects:	Biology Methods Temperature FRET RNA structure CD spectroscopy
ISSN:	2331-8325, 2331-8325
Online Access:	Get full text
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