Merkel cell polyomavirus DNA sequences in the blood of healthy population of Pakistan

This study aimed at detecting and quantifying Merkel cell polyomavirus (MCPyV) viral loads in the peripheral blood of healthy Pakistani individuals. A total of 221 whole blood samples obtained from healthy individuals were examined by qPCR. MCPyV was detected in the peripheral blood of 31.2% healthy...

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Vydáno v:Future microbiology Ročník 14; číslo 7; s. 599 - 608
Hlavní autoři: Shahzad, Naveed, Hussain, Iqra, Gilani, Usman S, Tayyeb, Asima, Aslam, Muhammad A, Khurshid, Muhammad, Hassan, Umair, Tasneem, Fareeda, Umer, Muhammd, Rashid, Naeem
Médium: Journal Article
Jazyk:angličtina
Vydáno: England Future Medicine Ltd 01.05.2019
Témata:
Age groups
Antigens
Blood & organ donations
Blood cells
Deoxyribonucleic acid
DNA
Genetic testing
Hepatitis
Immunocompetence
Immunocompetent individuals
Infections
MCPyV
Nucleotide sequence
Pakistan
Peripheral blood
Population
Punjab
qPCR
Skin cancer
Tumors
Urine
Viruses
Pakistan
Punjab
qPCR
Pakistan
MCPyV
Immunocompetent individuals
ISSN:1746-0913, 1746-0921, 1746-0921
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Abstract This study aimed at detecting and quantifying Merkel cell polyomavirus (MCPyV) viral loads in the peripheral blood of healthy Pakistani individuals. A total of 221 whole blood samples obtained from healthy individuals were examined by qPCR. MCPyV was detected in the peripheral blood of 31.2% healthy individuals. The rate of MCPyV positivity decreased from young (36%), to middle (33.7%) and elder (25.3%) age groups. Our data revealed higher prevalence of MCPyV in women (43.93%) than men (25.80%). The MCPyV viral load was calculated in the range of 0.06 -11 copies/ng of isolated DNA. The MCPyV viral load increased from young (median = 3.35) to elder (median = 5.66) age groups. The MCPyV circulate at a higher frequency by residing dormant in certain blood cells, which might act as potential vehicles for the spread of MCPyV infection among general population.
AbstractList Aim: This study aimed at detecting and quantifying Merkel cell polyomavirus (MCPyV) viral loads in the peripheral blood of healthy Pakistani individuals. Patients & methods: A total of 221 whole blood samples obtained from healthy individuals were examined by qPCR. Results & conclusion: MCPyV was detected in the peripheral blood of 31.2% healthy individuals. The rate of MCPyV positivity decreased from young (36%), to middle (33.7%) and elder (25.3%) age groups. Our data revealed higher prevalence of MCPyV in women (43.93%) than men (25.80%). The MCPyV viral load was calculated in the range of 0.06 –11 copies/ng of isolated DNA. The MCPyV viral load increased from young (median = 3.35) to elder (median = 5.66) age groups. The MCPyV circulate at a higher frequency by residing dormant in certain blood cells, which might act as potential vehicles for the spread of MCPyV infection among general population.
This study aimed at detecting and quantifying Merkel cell polyomavirus (MCPyV) viral loads in the peripheral blood of healthy Pakistani individuals. A total of 221 whole blood samples obtained from healthy individuals were examined by qPCR. MCPyV was detected in the peripheral blood of 31.2% healthy individuals. The rate of MCPyV positivity decreased from young (36%), to middle (33.7%) and elder (25.3%) age groups. Our data revealed higher prevalence of MCPyV in women (43.93%) than men (25.80%). The MCPyV viral load was calculated in the range of 0.06 -11 copies/ng of isolated DNA. The MCPyV viral load increased from young (median = 3.35) to elder (median = 5.66) age groups. The MCPyV circulate at a higher frequency by residing dormant in certain blood cells, which might act as potential vehicles for the spread of MCPyV infection among general population.
This study aimed at detecting and quantifying Merkel cell polyomavirus (MCPyV) viral loads in the peripheral blood of healthy Pakistani individuals. A total of 221 whole blood samples obtained from healthy individuals were examined by qPCR. MCPyV was detected in the peripheral blood of 31.2% healthy individuals. The rate of MCPyV positivity decreased from young (36%), to middle (33.7%) and elder (25.3%) age groups. Our data revealed higher prevalence of MCPyV in women (43.93%) than men (25.80%). The MCPyV viral load was calculated in the range of 0.06 -11 copies/ng of isolated DNA. The MCPyV viral load increased from young (median = 3.35) to elder (median = 5.66) age groups. The MCPyV circulate at a higher frequency by residing dormant in certain blood cells, which might act as potential vehicles for the spread of MCPyV infection among general population.
Aim: This study aimed at detecting and quantifying Merkel cell polyomavirus (MCPyV) viral loads in the peripheral blood of healthy Pakistani individuals. Patients & methods: A total of 221 whole blood samples obtained from healthy individuals were examined by qPCR. Results & conclusion: MCPyV was detected in the peripheral blood of 31.2% healthy individuals. The rate of MCPyV positivity decreased from young (36%), to middle (33.7%) and elder (25.3%) age groups. Our data revealed higher prevalence of MCPyV in women (43.93%) than men (25.80%). The MCPyV viral load was calculated in the range of 0.06 -11 copies/ng of isolated DNA. The MCPyV viral load increased from young (median = 3.35) to elder (median = 5.66) age groups. The MCPyV circulate at a higher frequency by residing dormant in certain blood cells, which might act as potential vehicles for the spread of MCPyV infection among general population.Aim: This study aimed at detecting and quantifying Merkel cell polyomavirus (MCPyV) viral loads in the peripheral blood of healthy Pakistani individuals. Patients & methods: A total of 221 whole blood samples obtained from healthy individuals were examined by qPCR. Results & conclusion: MCPyV was detected in the peripheral blood of 31.2% healthy individuals. The rate of MCPyV positivity decreased from young (36%), to middle (33.7%) and elder (25.3%) age groups. Our data revealed higher prevalence of MCPyV in women (43.93%) than men (25.80%). The MCPyV viral load was calculated in the range of 0.06 -11 copies/ng of isolated DNA. The MCPyV viral load increased from young (median = 3.35) to elder (median = 5.66) age groups. The MCPyV circulate at a higher frequency by residing dormant in certain blood cells, which might act as potential vehicles for the spread of MCPyV infection among general population.
Author Umer, Muhammd
Rashid, Naeem
Hussain, Iqra
Aslam, Muhammad A
Khurshid, Muhammad
Tasneem, Fareeda
Hassan, Umair
Shahzad, Naveed
Gilani, Usman S
Tayyeb, Asima
AuthorAffiliation 2Institute of Microbiology, University of Agriculture Faisalabad, Pakistan
1School of Biological Sciences, University of the Punjab, Lahore, Pakistan
4Department of Zoology, University of the Punjab, Lahore, Pakistan
5Queensland Micro- & Nanotechnology Centre (QMNC), Griffith University, Nathan Campus, QLD 4111, Australia
3Institute of Biochemistry & Biotechnology, University of the Punjab, Lahore, Pakistan
AuthorAffiliation_xml – name: 1School of Biological Sciences, University of the Punjab, Lahore, Pakistan
– name: 2Institute of Microbiology, University of Agriculture Faisalabad, Pakistan
– name: 4Department of Zoology, University of the Punjab, Lahore, Pakistan
– name: 3Institute of Biochemistry & Biotechnology, University of the Punjab, Lahore, Pakistan
– name: 5Queensland Micro- & Nanotechnology Centre (QMNC), Griffith University, Nathan Campus, QLD 4111, Australia
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/30864465$$D View this record in MEDLINE/PubMed
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CitedBy_id crossref_primary_10_1016_j_meegid_2020_104563
crossref_primary_10_3389_fimmu_2021_738486
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Snippet This study aimed at detecting and quantifying Merkel cell polyomavirus (MCPyV) viral loads in the peripheral blood of healthy Pakistani individuals. A total of...
Aim: This study aimed at detecting and quantifying Merkel cell polyomavirus (MCPyV) viral loads in the peripheral blood of healthy Pakistani individuals....
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SubjectTerms Age groups
Antigens
Blood & organ donations
Blood cells
Deoxyribonucleic acid
DNA
Genetic testing
Hepatitis
Immunocompetence
Immunocompetent individuals
Infections
MCPyV
Nucleotide sequence
Pakistan
Peripheral blood
Population
Punjab
qPCR
Skin cancer
Tumors
Urine
Viruses
Title Merkel cell polyomavirus DNA sequences in the blood of healthy population of Pakistan
URI http://dx.doi.org/10.2217/fmb-2018-0314
https://www.ncbi.nlm.nih.gov/pubmed/30864465
https://www.proquest.com/docview/2276745740
https://www.proquest.com/docview/2191010856
Volume 14
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