Endotoxin-polluted medium in a human in vitro fertilization program

Optimal quality control measures to obtain the highest possible success rate for an in vitro fertilization and embryo transfer program is important in any program. Poor results obtained during a cycle where no pregnancies were achieved necessitated an investigation into possible causes. A suspected...

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Bibliographic Details
Published in:	Fertility and sterility Vol. 46; no. 2; p. 273
Main Authors:	Snyman, E, Van der Merwe, J V
Format:	Journal Article
Language:	English
Published:	United States 01.08.1986
Subjects:	Culture Media - standards Embryo, Mammalian - drug effects Endotoxins - adverse effects Female Fertilization in Vitro - methods Humans Oocytes - drug effects Pregnancy Water
ISSN:	0015-0282
Online Access:	Get more information
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Summary:	Optimal quality control measures to obtain the highest possible success rate for an in vitro fertilization and embryo transfer program is important in any program. Poor results obtained during a cycle where no pregnancies were achieved necessitated an investigation into possible causes. A suspected contamination of the multidistilled water utilized in medium preparation was confirmed. During a successful cycle with no endotoxins present, a pregnancy rate of 27.2% was achieved. This is higher than the 6.3% pregnancy rate achieved in another cycle where endotoxins were isolated in the media. This article illustrates cell morphologic and ultrastructural changes which could be ascribed to the presence of these endotoxins.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0015-0282
DOI:	10.1016/S0015-0282(16)49525-7