Endotoxin-polluted medium in a human in vitro fertilization program

Optimal quality control measures to obtain the highest possible success rate for an in vitro fertilization and embryo transfer program is important in any program. Poor results obtained during a cycle where no pregnancies were achieved necessitated an investigation into possible causes. A suspected...

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Vydáno v:Fertility and sterility Ročník 46; číslo 2; s. 273
Hlavní autoři: Snyman, E, Van der Merwe, J V
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 01.08.1986
Témata:
Culture Media - standards
Embryo, Mammalian - drug effects
Endotoxins - adverse effects
Female
Fertilization in Vitro - methods
Humans
Oocytes - drug effects
Pregnancy
Water
ISSN:0015-0282
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Abstract Optimal quality control measures to obtain the highest possible success rate for an in vitro fertilization and embryo transfer program is important in any program. Poor results obtained during a cycle where no pregnancies were achieved necessitated an investigation into possible causes. A suspected contamination of the multidistilled water utilized in medium preparation was confirmed. During a successful cycle with no endotoxins present, a pregnancy rate of 27.2% was achieved. This is higher than the 6.3% pregnancy rate achieved in another cycle where endotoxins were isolated in the media. This article illustrates cell morphologic and ultrastructural changes which could be ascribed to the presence of these endotoxins.
AbstractList Optimal quality control measures to obtain the highest possible success rate for an in vitro fertilization and embryo transfer program is important in any program. Poor results obtained during a cycle where no pregnancies were achieved necessitated an investigation into possible causes. A suspected contamination of the multidistilled water utilized in medium preparation was confirmed. During a successful cycle with no endotoxins present, a pregnancy rate of 27.2% was achieved. This is higher than the 6.3% pregnancy rate achieved in another cycle where endotoxins were isolated in the media. This article illustrates cell morphologic and ultrastructural changes which could be ascribed to the presence of these endotoxins.
Optimal quality control measures to obtain the highest possible success rate for an in vitro fertilization and embryo transfer program is important in any program. Poor results obtained during a cycle where no pregnancies were achieved necessitated an investigation into possible causes. A suspected contamination of the multidistilled water utilized in medium preparation was confirmed. During a successful cycle with no endotoxins present, a pregnancy rate of 27.2% was achieved. This is higher than the 6.3% pregnancy rate achieved in another cycle where endotoxins were isolated in the media. This article illustrates cell morphologic and ultrastructural changes which could be ascribed to the presence of these endotoxins.Optimal quality control measures to obtain the highest possible success rate for an in vitro fertilization and embryo transfer program is important in any program. Poor results obtained during a cycle where no pregnancies were achieved necessitated an investigation into possible causes. A suspected contamination of the multidistilled water utilized in medium preparation was confirmed. During a successful cycle with no endotoxins present, a pregnancy rate of 27.2% was achieved. This is higher than the 6.3% pregnancy rate achieved in another cycle where endotoxins were isolated in the media. This article illustrates cell morphologic and ultrastructural changes which could be ascribed to the presence of these endotoxins.
Author Snyman, E
Van der Merwe, J V
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StartPage 273
SubjectTerms Culture Media - standards
Embryo, Mammalian - drug effects
Endotoxins - adverse effects
Female
Fertilization in Vitro - methods
Humans
Oocytes - drug effects
Pregnancy
Water
Title Endotoxin-polluted medium in a human in vitro fertilization program
URI https://www.ncbi.nlm.nih.gov/pubmed/3732534
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