Functional, Non-Clonal IgMa-Restricted B Cell Receptor Interactions with the HIV-1 Envelope gp41 Membrane Proximal External Region

The membrane proximal external region (MPER) of HIV-1 gp41 has several features that make it an attractive antibody-based vaccine target, but eliciting an effective gp41 MPER-specific protective antibody response remains elusive. One fundamental issue is whether the failure to make gp41 MPER-specifi...

Celý popis

Uložené v:

Podrobná bibliografia
Vydané v:	PloS one Ročník 4; číslo 10; s. e7215
Hlavní autori:	Verkoczy, Laurent, Moody, M. Anthony, Holl, T. Matt, Bouton-Verville, Hilary, Scearce, Richard M., Hutchinson, Jennifer, Alam, S. Munir, Kelsoe, Garnett, Haynes, Barton F.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States Public Library of Science 06.10.2009 Public Library of Science (PLoS)
Predmet:	Animals Antibodies Antibody response Antigens Avidity B-Cell Activation Factor Receptor - metabolism B-cell receptor B-Lymphocytes - virology Binding CD79 Antigens - metabolism Cell activation Cell Membrane - metabolism Epitopes - chemistry Female Glycoprotein gp41 HIV HIV Envelope Protein gp41 - metabolism HIV-1 - metabolism Human immunodeficiency virus Immunoglobulin M Immunoglobulins Immunological tolerance Immunology Immunology/Genetics of the Immune System Immunology/Immune Response Immunology/Immunity to Infections Immunology/Immunomodulation Immunology/Leukocyte Signaling and Gene Expression Lymphocyte Activation Lymphocyte receptors Lymphocytes B Mice Mice, Inbred BALB C Mice, Inbred C57BL Monoclonal antibodies Peptides Peritoneum Protein Binding Receptors Signal Transduction Signaling Spleen Spleen - metabolism Vaccines Virology North Carolina United States > US
ISSN:	1932-6203, 1932-6203
On-line prístup:	Získať plný text
Tagy:	Pridať tag Žiadne tagy, Buďte prvý, kto otaguje tento záznam!

Abstract	The membrane proximal external region (MPER) of HIV-1 gp41 has several features that make it an attractive antibody-based vaccine target, but eliciting an effective gp41 MPER-specific protective antibody response remains elusive. One fundamental issue is whether the failure to make gp41 MPER-specific broadly neutralizing antibodies like 2F5 and 4E10 is due to structural constraints with the gp41 MPER, or alternatively, if gp41 MPER epitope-specific B cells are lost to immunological tolerance. An equally important question is how B cells interact with, and respond to, the gp41 MPER epitope, including whether they engage this epitope in a non-canonical manner i.e., by non-paratopic recognition via B cell receptors (BCR). To begin understanding how B cells engage the gp41 MPER, we characterized B cell-gp41 MPER interactions in BALB/c and C57BL/6 mice. Surprisingly, we found that a significant (approximately 7%) fraction of splenic B cells from BALB/c, but not C57BL/6 mice, bound the gp41 MPER via their BCRs. This strain-specific binding was concentrated in IgM(hi) subsets, including marginal zone and peritoneal B1 B cells, and correlated with enriched fractions (approximately 15%) of gp41 MPER-specific IgM secreted by in vitro-activated splenic B cells. Analysis of Igh(a) (BALB/c) and Igh(b) (C57BL/6) congenic mice demonstrated that gp41 MPER binding was controlled by determinants of the Igh(a) locus. Mapping of MPER gp41 interactions with IgM(a) identified MPER residues distinct from those to which mAb 2F5 binds and demonstrated the requirement of Fc C(H) regions. Importantly, gp41 MPER ligation produced detectable BCR-proximal signaling events, suggesting that interactions between gp41 MPER and IgM(a) determinants may elicit partial B cell activation. These data suggest that low avidity, non-paratopic interactions between the gp41 MPER and membrane Ig on naïve B cells may interfere with or divert bnAb responses.
AbstractList	The membrane proximal external region (MPER) of HIV-1 gp41 has several features that make it an attractive antibody-based vaccine target, but eliciting an effective gp41 MPER-specific protective antibody response remains elusive. One fundamental issue is whether the failure to make gp41 MPER-specific broadly neutralizing antibodies like 2F5 and 4E10 is due to structural constraints with the gp41 MPER, or alternatively, if gp41 MPER epitope-specific B cells are lost to immunological tolerance. An equally important question is how B cells interact with, and respond to, the gp41 MPER epitope, including whether they engage this epitope in a non-canonical manner i.e., by non-paratopic recognition via B cell receptors (BCR). To begin understanding how B cells engage the gp41 MPER, we characterized B cell-gp41 MPER interactions in BALB/c and C57BL/6 mice. Surprisingly, we found that a significant (approximately 7%) fraction of splenic B cells from BALB/c, but not C57BL/6 mice, bound the gp41 MPER via their BCRs. This strain-specific binding was concentrated in IgM(hi) subsets, including marginal zone and peritoneal B1 B cells, and correlated with enriched fractions (approximately 15%) of gp41 MPER-specific IgM secreted by in vitro-activated splenic B cells. Analysis of Igh(a) (BALB/c) and Igh(b) (C57BL/6) congenic mice demonstrated that gp41 MPER binding was controlled by determinants of the Igh(a) locus. Mapping of MPER gp41 interactions with IgM(a) identified MPER residues distinct from those to which mAb 2F5 binds and demonstrated the requirement of Fc C(H) regions. Importantly, gp41 MPER ligation produced detectable BCR-proximal signaling events, suggesting that interactions between gp41 MPER and IgM(a) determinants may elicit partial B cell activation. These data suggest that low avidity, non-paratopic interactions between the gp41 MPER and membrane Ig on naïve B cells may interfere with or divert bnAb responses. The membrane proximal external region (MPER) of HIV-1 gp41 has several features that make it an attractive antibody-based vaccine target, but eliciting an effective gp41 MPER-specific protective antibody response remains elusive. One fundamental issue is whether the failure to make gp41 MPER-specific broadly neutralizing antibodies like 2F5 and 4E10 is due to structural constraints with the gp41 MPER, or alternatively, if gp41 MPER epitope-specific B cells are lost to immunological tolerance. An equally important question is how B cells interact with, and respond to, the gp41 MPER epitope, including whether they engage this epitope in a non-canonical manner i.e., by non-paratopic recognition via B cell receptors (BCR). To begin understanding how B cells engage the gp41 MPER, we characterized B cell-gp41 MPER interactions in BALB/c and C57BL/6 mice. Surprisingly, we found that a significant (approximately 7%) fraction of splenic B cells from BALB/c, but not C57BL/6 mice, bound the gp41 MPER via their BCRs. This strain-specific binding was concentrated in IgM(hi) subsets, including marginal zone and peritoneal B1 B cells, and correlated with enriched fractions (approximately 15%) of gp41 MPER-specific IgM secreted by in vitro-activated splenic B cells. Analysis of Igh(a) (BALB/c) and Igh(b) (C57BL/6) congenic mice demonstrated that gp41 MPER binding was controlled by determinants of the Igh(a) locus. Mapping of MPER gp41 interactions with IgM(a) identified MPER residues distinct from those to which mAb 2F5 binds and demonstrated the requirement of Fc C(H) regions. Importantly, gp41 MPER ligation produced detectable BCR-proximal signaling events, suggesting that interactions between gp41 MPER and IgM(a) determinants may elicit partial B cell activation. These data suggest that low avidity, non-paratopic interactions between the gp41 MPER and membrane Ig on naïve B cells may interfere with or divert bnAb responses.The membrane proximal external region (MPER) of HIV-1 gp41 has several features that make it an attractive antibody-based vaccine target, but eliciting an effective gp41 MPER-specific protective antibody response remains elusive. One fundamental issue is whether the failure to make gp41 MPER-specific broadly neutralizing antibodies like 2F5 and 4E10 is due to structural constraints with the gp41 MPER, or alternatively, if gp41 MPER epitope-specific B cells are lost to immunological tolerance. An equally important question is how B cells interact with, and respond to, the gp41 MPER epitope, including whether they engage this epitope in a non-canonical manner i.e., by non-paratopic recognition via B cell receptors (BCR). To begin understanding how B cells engage the gp41 MPER, we characterized B cell-gp41 MPER interactions in BALB/c and C57BL/6 mice. Surprisingly, we found that a significant (approximately 7%) fraction of splenic B cells from BALB/c, but not C57BL/6 mice, bound the gp41 MPER via their BCRs. This strain-specific binding was concentrated in IgM(hi) subsets, including marginal zone and peritoneal B1 B cells, and correlated with enriched fractions (approximately 15%) of gp41 MPER-specific IgM secreted by in vitro-activated splenic B cells. Analysis of Igh(a) (BALB/c) and Igh(b) (C57BL/6) congenic mice demonstrated that gp41 MPER binding was controlled by determinants of the Igh(a) locus. Mapping of MPER gp41 interactions with IgM(a) identified MPER residues distinct from those to which mAb 2F5 binds and demonstrated the requirement of Fc C(H) regions. Importantly, gp41 MPER ligation produced detectable BCR-proximal signaling events, suggesting that interactions between gp41 MPER and IgM(a) determinants may elicit partial B cell activation. These data suggest that low avidity, non-paratopic interactions between the gp41 MPER and membrane Ig on naïve B cells may interfere with or divert bnAb responses. The membrane proximal external region (MPER) of HIV-1 gp41 has several features that make it an attractive antibody-based vaccine target, but eliciting an effective gp41 MPER-specific protective antibody response remains elusive. One fundamental issue is whether the failure to make gp41 MPER-specific broadly neutralizing antibodies like 2F5 and 4E10 is due to structural constraints with the gp41 MPER, or alternatively, if gp41 MPER epitope-specific B cells are lost to immunological tolerance. An equally important question is how B cells interact with, and respond to, the gp41 MPER epitope, including whether they engage this epitope in a non-canonical manner i.e., by non-paratopic recognition via B cell receptors (BCR). To begin understanding how B cells engage the gp41 MPER, we characterized B cell-gp41 MPER interactions in BALB/c and C57BL/6 mice. Surprisingly, we found that a significant (∼7%) fraction of splenic B cells from BALB/c, but not C57BL/6 mice, bound the gp41 MPER via their BCRs. This strain-specific binding was concentrated in IgMhi subsets, including marginal zone and peritoneal B1 B cells, and correlated with enriched fractions (∼15%) of gp41 MPER-specific IgM secreted by in vitro-activated splenic B cells. Analysis of Igha (BALB/c) and Ighb (C57BL/6) congenic mice demonstrated that gp41 MPER binding was controlled by determinants of the Igha locus. Mapping of MPER gp41 interactions with IgMa identified MPER residues distinct from those to which mAb 2F5 binds and demonstrated the requirement of Fc CH regions. Importantly, gp41 MPER ligation produced detectable BCR-proximal signaling events, suggesting that interactions between gp41 MPER and IgMa determinants may elicit partial B cell activation. These data suggest that low avidity, non-paratopic interactions between the gp41 MPER and membrane Ig on naïve B cells may interfere with or divert bnAb responses.
Author	Verkoczy, Laurent Alam, S. Munir Kelsoe, Garnett Hutchinson, Jennifer Bouton-Verville, Hilary Holl, T. Matt Scearce, Richard M. Haynes, Barton F. Moody, M. Anthony
AuthorAffiliation	New York University School of Medicine, United States of America 1 Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, United States of America 2 Department of Immunology, Duke University Medical Center, Durham, North Carolina, United States of America
AuthorAffiliation_xml	– name: New York University School of Medicine, United States of America – name: 1 Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, United States of America – name: 2 Department of Immunology, Duke University Medical Center, Durham, North Carolina, United States of America
Author_xml	– sequence: 1 givenname: Laurent surname: Verkoczy fullname: Verkoczy, Laurent – sequence: 2 givenname: M. Anthony surname: Moody fullname: Moody, M. Anthony – sequence: 3 givenname: T. Matt surname: Holl fullname: Holl, T. Matt – sequence: 4 givenname: Hilary surname: Bouton-Verville fullname: Bouton-Verville, Hilary – sequence: 5 givenname: Richard M. surname: Scearce fullname: Scearce, Richard M. – sequence: 6 givenname: Jennifer surname: Hutchinson fullname: Hutchinson, Jennifer – sequence: 7 givenname: S. Munir surname: Alam fullname: Alam, S. Munir – sequence: 8 givenname: Garnett surname: Kelsoe fullname: Kelsoe, Garnett – sequence: 9 givenname: Barton F. surname: Haynes fullname: Haynes, Barton F.
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/19806186$$D View this record in MEDLINE/PubMed
BookMark	eNp9kstu1DAUhiNURC_wBggssWBDBt9iOyyQYDSlkVpAI2BrOc5JJqNMHOxMKVueHE8nrdoKsbJ1_P-fz-04OehdD0nynOAZYZK8Xbut7003G2J4hjGWlGSPkiOSM5oKitnBnfthchzCGuOMKSGeJIckV1gQJY6SP6fb3o6ti6Q36LPr03m3u6OiuTDpEsLoWztChT6iOXQdWoKFYXQeFf0I3lw7A_rVjis0rgCdFT9Sghb9JXRuANQMnKAL2JTe9IC-enfVbiJ7cRW9u0-W0ET_0-RxbboAz6bzJPl-uvg2P0vPv3wq5h_OU8skzlJTYSsNCMyNqCulSqpyC7XKpc3AKlpiSji3LNZYsxJnsUkGrKxJbhiRpWInycs9d-hc0FP7giY0pwxnWcajotgrKmfWevAxW_9bO9Pq64DzjTZ-bG0HmleVZYSUJReUS0lUKa0gvMKGG1xTGlnvp9-25QYqC_3oTXcPev-lb1e6cZeayowoIiLg9QTw7uc2TkJv2mDjEGIv3TZoyZjIVU5JVL56oPx3cS_uJnSbyc0uRMG7vcB6F4KHWtt2NLsJx_zaThOsd4t3A9e7xdPT4kUzf2C-5f_P9hflGd9s
CitedBy_id	crossref_primary_10_1016_j_jviromet_2010_10_022 crossref_primary_10_1111_j_1747_0285_2012_01423_x crossref_primary_10_1084_jem_20110363 crossref_primary_10_4049_jimmunol_2100172 crossref_primary_10_1371_journal_pone_0023532 crossref_primary_10_4049_jimmunol_1302829 crossref_primary_10_1126_scitranslmed_aaf0618 crossref_primary_10_1016_j_coi_2011_04_003 crossref_primary_10_1371_journal_pone_0085098 crossref_primary_10_1111_imr_12504 crossref_primary_10_1007_s00018_021_04009_z crossref_primary_10_4049_jimmunol_1101633 crossref_primary_10_4049_jimmunol_1300770 crossref_primary_10_1126_sciimmunol_aag0851 crossref_primary_10_4049_jimmunol_1302511 crossref_primary_10_1371_journal_ppat_1005042 crossref_primary_10_4049_jimmunol_1300971
Cites_doi	10.1016/S0264-410X(00)00267-X 10.1016/j.immuni.2007.11.018 10.1111/j.1365-3083.1993.tb01734.x 10.3109/08830189709116524 10.1038/31514 10.1128/JVI.75.18.8434-8439.2001 10.1099/0022-1317-77-9-2001 10.1084/jem.190.1.75 10.1128/JVI.75.24.12198-12208.2001 10.1007/s00281-004-0190-2 10.1089/aid.2006.22.998 10.3233/HAB-2005-143-403 10.1093/intimm/12.3.305 10.4049/jimmunol.154.12.6437 10.1146/annurev.biochem.70.1.777 10.1038/nature01470 10.1038/301720a0 10.1111/j.1600-065X.2000.imr017515.x 10.1089/08892220152741450 10.1073/pnas.0630530100 10.1126/science.274.5284.94 10.1073/pnas.84.5.1379 10.1128/JVI.71.4.2674-2684.1997 10.1038/nature01188 10.1038/387426a0 10.1016/S0022-1759(02)00499-4 10.1038/72318 10.1002/eji.1830191122 10.1038/nri799 10.1016/j.jinf.2004.04.007 10.1038/nri1853 10.1073/pnas.94.4.1356 10.1074/jbc.M205862200 10.1016/S0264-410X(99)00125-5 10.1128/JVI.02588-06 10.1128/JVI.75.22.10892-10905.2001 10.1016/j.immuni.2007.12.005 10.4049/jimmunol.164.10.5482 10.1089/088922200750054756 10.1128/JVI.79.2.1154-1163.2005 10.1034/j.1399-3011.2002.02988.x 10.1016/S1074-7613(00)80157-0 10.3233/HAB-2005-143-402 10.1073/pnas.0409217102 10.1084/jem.192.1.87 10.1146/annurev.iy.13.040195.002331 10.1016/j.virol.2006.04.043 10.1016/S0952-7915(00)00204-1 10.1016/S1074-7613(01)00135-2 10.1128/JVI.00927-07 10.4049/jimmunol.167.12.6834 10.1126/science.1111781 10.1016/j.virol.2005.08.042 10.1038/ni0304-233 10.4049/jimmunol.178.6.3593 10.4049/jimmunol.172.2.762 10.1128/JVI.79.2.1252-1261.2005 10.1126/science.177.4044.178 10.1128/JVI.73.5.4009-4018.1999 10.4049/jimmunol.178.7.4424 10.1128/JVI.67.11.6642-6647.1993 10.1073/pnas.94.5.1955 10.4049/jimmunol.137.1.28 10.4049/jimmunol.176.7.3931 10.1128/JVI.75.22.10906-10911.2001 10.1086/516443 10.1016/j.immuni.2004.12.011 10.1128/JVI.69.9.5723-5733.1995 10.1084/jem.188.8.1453 10.4049/jimmunol.161.12.6681 10.1128/MMBR.00020-07 10.1038/31405 10.1016/S0022-2836(03)00611-9 10.1128/JVI.78.19.10724-10737.2004 10.1586/14760584.5.4.579 10.1089/aid.1994.10.359
ContentType	Journal Article
Copyright	2009 Verkoczy et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Verkoczy et al. 2009
Copyright_xml	– notice: 2009 Verkoczy et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: Verkoczy et al. 2009
DBID	AAYXX CITATION CGR CUY CVF ECM EIF NPM 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS PTHSS PYCSY RC3 7X8 5PM DOA
DOI	10.1371/journal.pone.0007215
DatabaseName	CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Materials Science & Engineering ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland Advanced Technologies & Computer Science Collection Agricultural & Environmental Science Database ProQuest Central Essentials Biological Science Database ProQuest Central Technology Collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One ProQuest Materials Science Collection ProQuest Central Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection ProQuest Biological Science Collection Agricultural Science Database Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database (subscripiton) Materials Science Collection ProQuest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China Engineering collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) Directory of Open Access Journals
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central ProQuest Health & Medical Research Collection Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic
DatabaseTitleList	MEDLINE MEDLINE - Academic Agricultural Science Database
Database_xml	– sequence: 1 dbid: DOA name: DOAJ Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Sciences (General)
DocumentTitleAlternate	IgMa-Restricted HIV-1 Binding
EISSN	1932-6203
ExternalDocumentID	1292305554 oai_doaj_org_article_4ddc311bb46247718b7c614d0a4a0f22 PMC2751816 2898410951 19806186 10_1371_journal_pone_0007215
Genre	Research Support, Non-U.S. Gov't Journal Article
GeographicLocations	North Carolina United States--US
GeographicLocations_xml	– name: North Carolina – name: United States--US
GroupedDBID	--- 123 29O 2WC 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ AAUCC AAWOE AAYXX ABDBF ABIVO ABJCF ABUWG ACCTH ACGFO ACIHN ACIWK ACPRK ACUHS ADBBV ADRAZ AEAQA AENEX AEUYN AFFHD AFKRA AFPKN AFRAH AHMBA ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAWUL BBNVY BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CITATION CS3 D1I D1J D1K DIK DU5 E3Z EAP EAS EBD EMOBN ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IGS IHR IHW INH INR IOV IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ O5R O5S OK1 OVT P2P P62 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PPXIY PQGLB PQQKQ PROAC PSQYO PTHSS PYCSY RNS RPM SV3 TR2 UKHRP WOQ WOW ~02 ~KM 3V. ALIPV BBORY CGR CUY CVF ECM EIF NPM 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K DWQXO FR3 GNUQQ H94 K9. KL. M7N P64 PKEHL PQEST PQUKI PRINS RC3 7X8 ESTFP PUEGO 5PM - 02 AAPBV ABPTK ADACO BBAFP KM
ID	FETCH-LOGICAL-c3705-ad0c7ae604a6fd88b289cef897c5ec82b02144c3538f3b05137aec7f19a317b83
IEDL.DBID	7RV
ISSN	1932-6203
IngestDate	Fri Nov 26 17:12:06 EST 2021 Fri Oct 03 12:52:50 EDT 2025 Tue Nov 04 01:50:40 EST 2025 Mon Sep 08 09:24:10 EDT 2025 Sat Nov 29 14:25:22 EST 2025 Wed Feb 19 02:33:34 EST 2025 Tue Nov 18 22:28:54 EST 2025 Sat Nov 29 03:07:56 EST 2025
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	10
Language	English
License	This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. Creative Commons Attribution License
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c3705-ad0c7ae604a6fd88b289cef897c5ec82b02144c3538f3b05137aec7f19a317b83
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Conceived and designed the experiments: LKV GHK BFH. Performed the experiments: TMH HBV RMS JH SMA. Analyzed the data: LKV TMH HBV SMA GHK BFH. Contributed reagents/materials/analysis tools: MAM BFH. Wrote the paper: LKV BFH. Played a key role in revising the paper: GHK.
OpenAccessLink	https://www.proquest.com/docview/1292305554?pq-origsite=%requestingapplication%
PMID	19806186
PQID	1292305554
PQPubID	1436336
ParticipantIDs	plos_journals_1292305554 doaj_primary_oai_doaj_org_article_4ddc311bb46247718b7c614d0a4a0f22 pubmedcentral_primary_oai_pubmedcentral_nih_gov_2751816 proquest_miscellaneous_733698921 proquest_journals_1292305554 pubmed_primary_19806186 crossref_citationtrail_10_1371_journal_pone_0007215 crossref_primary_10_1371_journal_pone_0007215
PublicationCentury	2000
PublicationDate	20091006
PublicationDateYYYYMMDD	2009-10-06
PublicationDate_xml	– month: 10 year: 2009 text: 20091006 day: 6
PublicationDecade	2000
PublicationPlace	United States
PublicationPlace_xml	– name: United States – name: San Francisco – name: San Francisco, USA
PublicationTitle	PloS one
PublicationTitleAlternate	PLoS One
PublicationYear	2009
Publisher	Public Library of Science Public Library of Science (PLoS)
Publisher_xml	– name: Public Library of Science – name: Public Library of Science (PLoS)
References	MB Zwick (ref20) 2005; 79 F Martin (ref73) 2000; 12 MA Moody (ref39) 2008 F Martin (ref46) 2001; 13 F Loder (ref72) 1999; 190 MB Zwick (ref21) 2001; 75 B Blomberg (ref66) 1972; 177 JG Joyce (ref25) 2002; 277 RW Scamurra (ref60) 2000; 164 PL Earl (ref28) 1997; 71 T Muster (ref17) 1993; 67 DM Eckert (ref29) 2001; 70 B Gaynor (ref81) 1997; 94 CE Parker (ref43) 2001; 75 JR Mascola (ref13) 2000; 6 Y Ueda (ref44) 2007; 178 SM Alam (ref40) 2008; 82 MB Zwick (ref22) 2001; 75 J Newman (ref47) 2003; 272 GP Silverman (ref64) 1998; 192 G Barbato (ref14) 2003; 330 S Muller (ref59) 1993; 38 RM Cardoso (ref16) 2005; 22 A Buchacher (ref15) 1994; 10 E Coeffier (ref23) 2000; 19 M Montero (ref50) 2008; 72 JD Nelson (ref4) 2007; 81 CA Pennell (ref65) 1989; 19 DD Richman (ref10) 2003; 100 BF Haynes (ref32) 2005; 308 L Gorelik (ref75) 2004; 172 X Wei (ref7) 2003; 422 B He (ref38) 2006; 176 S Muller (ref57) 1997; 14 G Morahan (ref63) 1983; 301 A Cariappa (ref71) 2001; 14 BF Haynes (ref11) 2006; 5 C Marusic (ref27) 2001; 75 EK Bikoff (ref69) 1991; 21 S Karray (ref55) 1997; 94 PD Kwong (ref6) 1998; 393 S Sanchez-Martinez (ref33) 2006; 22 JS Rice (ref48) 2005; 102 S Karray (ref54) 1998; 161 DR Burton (ref9) 2004; 5 JD Altman (ref41) 1996; 274 F Martin (ref51) 2002; 2 H Acha-Orbea (ref67) 1995; 13 Q Chang (ref58) 2004; 49 L Eckhart (ref24) 1996; 77 (Pt 9) P Roben (ref62) 1995; 154 K Hayakawa (ref76) 1987; 84 JR Mascola (ref12) 1999; 73 F Gao (ref3) 2005; 79 X Liang (ref26) 1999; 17 F Martin (ref45) 2000; 175 R Bures (ref1) 2000; 16 MP D'Souza (ref2) 1997; 175 H-X Liao (ref77) 2006; 353 G Stiegler (ref19) 2001; 17 BF Haynes (ref34) 2005; 14 PD Kwong (ref5) 2002; 420 R Wyatt (ref8) 1998; 393 SM Alam (ref31) 2007; 178 D Allman (ref74) 2001; 167 EK Bikoff (ref70) 1986; 137 GJ Silverman (ref37) 2006; 6 Z-Y Sun (ref36) 2008; 28 CS Goodyear (ref52) 2005; 26 A Forsgren (ref61) 1966; 97 W Weissenhorn (ref30) 1997; 387 Zolla-Pazner (ref49) 2005; 14 G Ofek (ref18) 2004; 78 Y Tian (ref42) 2002; 59 MN Neshat (ref56) 2000; 12 V Kouskoff (ref68) 1998; 188 R Wyatt (ref78) 1995; 69 BF Haynes (ref80) 2006; 345 L Berberian (ref53) 1994; 7 B Korber (ref79) 1998 BF Haynes (ref35) 2008; 28
References_xml	– volume: 19 start-page: 684 year: 2000 ident: ref23 article-title: Antigenicity and immunogenicity of the HIV-1 gp41 epitope ELDKWA inserted into permissive sites of the MalE protein. publication-title: Vaccine doi: 10.1016/S0264-410X(00)00267-X – volume: 28 start-page: 52 year: 2008 ident: ref36 article-title: HIV-1 broadly neutralizing antibody extracts its epitope from a kinked MPER segment on the viral membrane. publication-title: Immunity doi: 10.1016/j.immuni.2007.11.018 – volume: 38 start-page: 327 year: 1993 ident: ref59 article-title: B-cell abnormalities in AIDS: stable and clonally-restricted antibody response in HIV-1 infection. publication-title: Scand J Immunol doi: 10.1111/j.1365-3083.1993.tb01734.x – volume: 97 start-page: 822 year: 1966 ident: ref61 article-title: Protein A from S.aureus. I. Pseudoimmune reaction with human gamma globulin. publication-title: JImmunol – volume: 14 start-page: 339 year: 1997 ident: ref57 article-title: B cell superantigens in HIV-1 infection. publication-title: Int Rev Immunol doi: 10.3109/08830189709116524 – volume: 393 start-page: 705 year: 1998 ident: ref8 article-title: The antigenic structure of the HIV gp120 envelope glycoprotein. publication-title: Nature doi: 10.1038/31514 – volume: 75 start-page: 8434 year: 2001 ident: ref27 article-title: Chimeric plant virus particles as immunogens for inducing murine and human immune responses against human immunodeficiency virus type 1. publication-title: J Virol doi: 10.1128/JVI.75.18.8434-8439.2001 – volume: 77 (Pt 9) start-page: 2001 year: 1996 ident: ref24 article-title: Immunogenic presentation of a conserved gp41 epitope of human immunodeficiency virus type 1 on recombinant surface antigen of hepatitis B virus. publication-title: J Gen Virol doi: 10.1099/0022-1317-77-9-2001 – volume: 190 start-page: 75 year: 1999 ident: ref72 article-title: B cell devlopment in the spleen takes place in discrete steps and is determined by the quality of B cell receptor-derived signals. publication-title: J Exp Med doi: 10.1084/jem.190.1.75 – volume: 75 start-page: 12198 year: 2001 ident: ref22 article-title: Neutralization synergy of human immunodeficiency virus type 1 primary isolates by cocktails of broadly neutralizing antibodies. publication-title: J Virol doi: 10.1128/JVI.75.24.12198-12208.2001 – volume: 26 start-page: 463 year: 2005 ident: ref52 article-title: B cell superantigens: a microbe's answer to innate-like B cells and natural antibodies. publication-title: Springer Semin Immunopathol doi: 10.1007/s00281-004-0190-2 – volume: 22 start-page: 998 year: 2006 ident: ref33 article-title: Membrane association and epitope recognition by HIV-1 neutralizing anti-gp41 2F5 and 4E10 antibodies. publication-title: AIDS Res Hum Retroviruses doi: 10.1089/aid.2006.22.998 – volume: 14 start-page: 69 year: 2005 ident: ref49 article-title: Improving on nature: focusing the immune response on the V3 loop. publication-title: Hum Antibodies doi: 10.3233/HAB-2005-143-403 – volume: 12 start-page: 305 year: 2000 ident: ref56 article-title: Mapping the B cell superantigen binding site for HIV-1 gp120 on a V(H)3 Ig. publication-title: Int Immunol doi: 10.1093/intimm/12.3.305 – volume: 154 start-page: 6437 year: 1995 ident: ref62 article-title: VH3 antibodies bind domain D of staphyloccocal protein A. publication-title: JImmunol doi: 10.4049/jimmunol.154.12.6437 – volume: 70 start-page: 777 year: 2001 ident: ref29 article-title: Mechanisms of viral membrane fusion and its inhibition. publication-title: Annu Rev Biochem doi: 10.1146/annurev.biochem.70.1.777 – volume: 7 start-page: 641 year: 1994 ident: ref53 article-title: Effects of HIV infection on VH3 (D12 idiotope) B cells in vivo. publication-title: J Acquir Immune Defic Syndr – volume: 422 start-page: 307 year: 2003 ident: ref7 article-title: Antibody neutralization and escape by HIV-1. publication-title: Nature doi: 10.1038/nature01470 – volume: 301 start-page: 720 year: 1983 ident: ref63 article-title: An idiotypic determinant formed by both immunoglobulin constant and variable regions. publication-title: Nature doi: 10.1038/301720a0 – volume: 175 start-page: 70 year: 2000 ident: ref45 article-title: B-cell subsets and the mature preimmune repertoire. Marginal zone and B1 B cells as part of a “natural immune memory”. publication-title: Immunol Rev doi: 10.1111/j.1600-065X.2000.imr017515.x – volume: 17 start-page: 1757 year: 2001 ident: ref19 article-title: A potent cross-clade neutralizing human monoclonal antibody against a novel epitope on gp41 of human immunodeficiency virus type 1. publication-title: AIDS Res Hum Retroviruses doi: 10.1089/08892220152741450 – volume: 100 start-page: 4144 year: 2003 ident: ref10 article-title: Rapid evolution of the neutralizing antibody response to HIV type 1 infection. publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0630530100 – volume: 274 start-page: 94 year: 1996 ident: ref41 article-title: Phenotypic analysis of antigen-specific T lymphocytes. publication-title: Science doi: 10.1126/science.274.5284.94 – volume: 84 start-page: 1379 year: 1987 ident: ref76 article-title: Isolation of high-affinity memory B cells: phycoerythrin as a probe for antigen-binding cells. publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.84.5.1379 – volume: 71 start-page: 2674 year: 1997 ident: ref28 article-title: Epitope map of human immunodeficiency virus type 1 gp41 derived from 47 monoclonal antibodies produced by immunization with oligomeric envelope protein. publication-title: J Virol doi: 10.1128/JVI.71.4.2674-2684.1997 – volume: 420 start-page: 678 year: 2002 ident: ref5 article-title: HIV-1 evades antibody-mediated neutralization through conformational masking of receptor-binding sites. publication-title: Nature doi: 10.1038/nature01188 – volume: 387 start-page: 426 year: 1997 ident: ref30 article-title: Atomic structure of the ectodomain from HIV-1 gp41. publication-title: Nature doi: 10.1038/387426a0 – volume: 272 start-page: 177 year: 2003 ident: ref47 article-title: Identification of an antigen-specific B cell population. publication-title: J Immunol Methods doi: 10.1016/S0022-1759(02)00499-4 – volume: 6 start-page: 207 year: 2000 ident: ref13 article-title: Protection of macaques against vaginal transmission of a pathogenic HIV-1/SIV chimeric virus by passive infusion of neutralizing antibodies. publication-title: Nat Med doi: 10.1038/72318 – volume: 19 start-page: 2115 year: 1989 ident: ref65 article-title: Organization and expression of VH gene families preferentially expressed by Ly-1+ (CD5) B B cells. publication-title: Eur J immunol doi: 10.1002/eji.1830191122 – volume: 2 start-page: 323 year: 2002 ident: ref51 article-title: Marginal Zone B cells. publication-title: Nature Immunol. Rev doi: 10.1038/nri799 – volume: 49 start-page: 274 year: 2004 ident: ref58 article-title: VH3 gene expression in children with HIV infection. publication-title: J Infect doi: 10.1016/j.jinf.2004.04.007 – volume: 6 start-page: 465 year: 2006 ident: ref37 article-title: Confounding B-cell defences: lessons from a staphylococcal superantigen. publication-title: Nat Rev Immunol doi: 10.1038/nri1853 – volume: 94 start-page: 1356 year: 1997 ident: ref55 article-title: Identification of the B cell superantigen-binding site of HIV-1 gp120. publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.94.4.1356 – volume: 277 start-page: 45811 year: 2002 ident: ref25 article-title: Enhancement of alpha-helicity in the HIV-1 inhibitory peptide DP178 leads to an increased affinity for human monoclonal antibody 2F5 but does not elicit neutralizing responses in vitro. Implications for vaccine design. publication-title: J Biol Chem doi: 10.1074/jbc.M205862200 – volume: 17 start-page: 2862 year: 1999 ident: ref26 article-title: Epitope insertion into variable loops of HIV-1 gp120 as a potential means to improve immunogenicity of viral envelope protein. publication-title: Vaccine doi: 10.1016/S0264-410X(99)00125-5 – volume: 81 start-page: 4033 year: 2007 ident: ref4 article-title: An affinity-enhanced neutralizing antibody against the membrane-proximal external region of human immunodeficiency virus type 1 gp41 recognizes an epitope between those of 2F5 and 4E10. publication-title: J Virol doi: 10.1128/JVI.02588-06 – volume: 75 start-page: 10892 year: 2001 ident: ref21 article-title: Broadly neutralizing antibodies targeted to the membrane-proximal external region of human immunodeficiency virus type 1 glycoprotein gp41. publication-title: J Virol doi: 10.1128/JVI.75.22.10892-10905.2001 – volume: 28 start-page: 10 year: 2008 ident: ref35 article-title: HIV-1 Hides an Achilles' Heel in Virion Lipids. publication-title: Immunity doi: 10.1016/j.immuni.2007.12.005 – volume: 164 start-page: 5482 year: 2000 ident: ref60 article-title: Impact of HIV-1 infection on VH3 gene repertoire of naive human B cells. publication-title: J Immunol doi: 10.4049/jimmunol.164.10.5482 – volume: 16 start-page: 2019 year: 2000 ident: ref1 article-title: Immunization with recombinant canarypox vectors expressing membrane-anchored glycoprotein 120 followed by glycoprotein 160 boosting fails to generate antibodies that neutralize R5 primary isolates of human immunodeficiency virus type 1. publication-title: AIDS Res Hum Retroviruses doi: 10.1089/088922200750054756 – volume: 79 start-page: 1154 year: 2005 ident: ref3 article-title: Antigenicity and immunogenicity of a synthetic human immunodeficiency virus type 1 group m consensus envelope glycoprotein. publication-title: J Virol doi: 10.1128/JVI.79.2.1154-1163.2005 – volume: 59 start-page: 264 year: 2002 ident: ref42 article-title: Structure-affinity relationships in the gp41 ELDKWA epitope for the HIV-1 neutralizing monoclonal antibody 2F5: effects of side-chain and nackbone modifications and conformational constraints. publication-title: J Pept Res doi: 10.1034/j.1399-3011.2002.02988.x – volume: 12 start-page: 39 year: 2000 ident: ref73 article-title: Positive selection from newly formed to marginal zone B cells depends on the rate of clonal production, CD19, and Btk. publication-title: Immunity doi: 10.1016/S1074-7613(00)80157-0 – volume: 14 start-page: 59 year: 2005 ident: ref34 article-title: Antibody polyspecificity and neutralization of HIV-1: a hypothesis. publication-title: Hum Antibodies doi: 10.3233/HAB-2005-143-402 – volume: 102 start-page: 1608 year: 2005 ident: ref48 article-title: Receptor editing in peripheral B cell tolerance. publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0409217102 – volume: 192 start-page: 87 year: 1998 ident: ref64 article-title: A B cell superantigen-induced persistent “Hole” in the B-1 repertoire. publication-title: J Exp Med doi: 10.1084/jem.192.1.87 – volume: 13 start-page: 459 year: 1995 ident: ref67 article-title: Superantigens of mouse mammary tumor virus. publication-title: Annual Rev Immunol doi: 10.1146/annurev.iy.13.040195.002331 – volume: 353 start-page: 268 year: 2006 ident: ref77 article-title: A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses. publication-title: Virology doi: 10.1016/j.virol.2006.04.043 – volume: 13 start-page: 195 year: 2001 ident: ref46 article-title: B1 cells: similarities and differences with other B cell subsets. publication-title: Curr Opin Immunol doi: 10.1016/S0952-7915(00)00204-1 – volume: 14 start-page: 603 year: 2001 ident: ref71 article-title: The follicular versus marginal zone B lymphocyte fate decision is regulated by Aiolos, Btk, and CD21. publication-title: Immunity doi: 10.1016/S1074-7613(01)00135-2 – volume: 82 start-page: 115 year: 2008 ident: ref40 article-title: Human immunodeficiency virus type 1 gp41 antibodies that mask membrane proximal region epitopes: antibody binding kinetics, induction, and potential for regulation in acute infection. publication-title: J Virol doi: 10.1128/JVI.00927-07 – volume: 167 start-page: 6834 year: 2001 ident: ref74 article-title: Resolution of three nonproliferative immature splenic B cell subsets reveals multiple selection points during peripheral B cell maturation. publication-title: J Immunol doi: 10.4049/jimmunol.167.12.6834 – volume: 308 start-page: 1906 year: 2005 ident: ref32 article-title: Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodies. publication-title: Science doi: 10.1126/science.1111781 – volume: 345 start-page: 44 year: 2006 ident: ref80 article-title: Analysis of HIV-1 subtype B third variable region peptide motifs for induction of neutralizing antibodies against HIV-1 primary isolates. publication-title: Virology doi: 10.1016/j.virol.2005.08.042 – volume: 5 start-page: 233 year: 2004 ident: ref9 article-title: HIV vaccine design and the neutralizing antibody problem. publication-title: Nat Immunol doi: 10.1038/ni0304-233 – volume: 178 start-page: 3593 year: 2007 ident: ref44 article-title: T-independent activation-induced cytidine deaminase expression, class-switch recombination, and antibody production by immature/transitional 1 B cells. publication-title: J Immunol doi: 10.4049/jimmunol.178.6.3593 – year: 2008 ident: ref39 article-title: Envelope epitope tetramers for identification and isolation of HIV-1 epitope-specific B lymphocytes. Nature Biotechnology. publication-title: Submitted – volume: 172 start-page: 762 year: 2004 ident: ref75 article-title: Cutting edge: BAFF regulates CD21/35 and CD23 expression independent of its B cell survival function. publication-title: J Immunol doi: 10.4049/jimmunol.172.2.762 – volume: 79 start-page: 1252 year: 2005 ident: ref20 article-title: Anti-human immunodeficiency virus type 1 (HIV-1) antibodies 2F5 and 4E10 require surprisingly few crucial residues in the membrane-proximal external region of glycoprotein gp41 to neutralize HIV-1. publication-title: J Virol doi: 10.1128/JVI.79.2.1252-1261.2005 – volume: 177 start-page: 178 year: 1972 ident: ref66 article-title: Genetics of the antibody response to dextran in mice. publication-title: Science doi: 10.1126/science.177.4044.178 – volume: 73 start-page: 4009 year: 1999 ident: ref12 article-title: Protection of Macaques against pathogenic simian/human immunodeficiency virus 89.6PD by passive transfer of neutralizing antibodies. publication-title: J Virol doi: 10.1128/JVI.73.5.4009-4018.1999 – volume: 178 start-page: 4424 year: 2007 ident: ref31 article-title: The role of antibody polyspecificity and lipid reactivity in binding of broadly neutralizing anti-HIV-1 envelope human monoclonal antibodies 2F5 and 4E10 to glycoprotein 41 membrane proximal envelope epitopes. publication-title: J Immunol doi: 10.4049/jimmunol.178.7.4424 – volume: 21 start-page: 1411 year: 1991 ident: ref69 article-title: Class II-restricted IgG2ab-specific T cells recognize a signal-minus form of the V-CH3b antigen. publication-title: JImmunol – volume: 67 start-page: 6642 year: 1993 ident: ref17 article-title: A conserved neutralizing epitope on gp41 of human immunodeficiency virus type 1. publication-title: J Virol doi: 10.1128/JVI.67.11.6642-6647.1993 – volume: 94 start-page: 1555 year: 1997 ident: ref81 article-title: Peptide inhibition of glomerular deposition of an anti-DNA antibody. publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.94.5.1955 – volume: 137 start-page: 28 year: 1986 ident: ref70 article-title: T cell clones specific for IgG2a of the a allotype:direct evidence for presentation of endogenous antigen. publication-title: J Immunol doi: 10.4049/jimmunol.137.1.28 – volume: 176 start-page: 3931 year: 2006 ident: ref38 article-title: HIV-1 envelope triggers polyclonal Ig class switch recombination through a CD40-independent mechanism involving BAFF and C-type lectin receptors. publication-title: J Immunol doi: 10.4049/jimmunol.176.7.3931 – volume: 75 start-page: 10906 year: 2001 ident: ref43 article-title: Fine definition of the gp41 epitope of the gp41 glycoprotein of human immunodeficiency virus type 1 for the neutralizing monoclonal 2F5. publication-title: J Virol doi: 10.1128/JVI.75.22.10906-10911.2001 – volume: 175 start-page: 1056 year: 1997 ident: ref2 article-title: Evaluation of monoclonal antibodies to human immunodeficiency virus type 1 primary isolates by neutralization assays: performance criteria for selecting candidate antibodies for clinical trials. AIDS Clinical Trials Group Antibody Selection Working Group. publication-title: J Infect Dis doi: 10.1086/516443 – volume: 22 start-page: 163 year: 2005 ident: ref16 article-title: Broadly neutralizing anti-HIV antibody 4E10 recognizes a helical conformation of a highly conserved fusion-associated motif in gp41. publication-title: Immunity doi: 10.1016/j.immuni.2004.12.011 – volume: 69 start-page: 5723 year: 1995 ident: ref78 article-title: Involvement of the V1/V2 variable loop structure in the exposure of human immunodeficiency virus type 1 gp120 envelopes by receptor binding. publication-title: J Virol doi: 10.1128/JVI.69.9.5723-5733.1995 – volume: 188 start-page: 1453 year: 1998 ident: ref68 article-title: Antigens varying in affinity for the B cell Receptor induce differential B lymphocyte responses. publication-title: J Exp Med doi: 10.1084/jem.188.8.1453 – volume: 161 start-page: 6681 year: 1998 ident: ref54 article-title: Structural basis of the gp120 superantigen-binding site on human immunoglobulins. publication-title: J Immunol doi: 10.4049/jimmunol.161.12.6681 – volume: 72 start-page: 54 year: 2008 ident: ref50 article-title: The membrane proximal external region of the human immunodeficiency virus type 1 envelope: dominant site of antibody neutralization and target for vaccine design. publication-title: Microbiol Mol Biol Rev doi: 10.1128/MMBR.00020-07 – volume: 393 start-page: 648 year: 1998 ident: ref6 article-title: Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody. publication-title: Nature doi: 10.1038/31405 – volume: 330 start-page: 1101 year: 2003 ident: ref14 article-title: Structural analysis of the epitope of the anti-HIV antibody 2F5 sheds light into its mechanism of neutralization and HIV fusion. publication-title: J Mol Biol doi: 10.1016/S0022-2836(03)00611-9 – volume: 78 start-page: 10724 year: 2004 ident: ref18 article-title: Structure and mechanistic analysis of the anti-human immunodeficiency virus type 1 antibody 2F5 in complex with its gp41 epitope. publication-title: J Virol doi: 10.1128/JVI.78.19.10724-10737.2004 – start-page: 102 year: 1998 ident: ref79 article-title: Numbering Positions in HIV Relative to HXB2CG. – volume: 5 start-page: 579 year: 2006 ident: ref11 article-title: Aiming to induce broadly reactive neutralizing antibody responses with HIV-1 vaccine candidates. publication-title: Expert Rev Vaccines doi: 10.1586/14760584.5.4.579 – volume: 10 start-page: 359 year: 1994 ident: ref15 article-title: Generation of human monoclonal antibodies against HIV-1 proteins; electrofusion and Epstein-Barr virus transformation for peripheral blood lymphocyte immortalization. publication-title: AIDS Res Hum Retroviruses doi: 10.1089/aid.1994.10.359
SSID	ssj0053866
Score	1.9248359
Snippet	The membrane proximal external region (MPER) of HIV-1 gp41 has several features that make it an attractive antibody-based vaccine target, but eliciting an...
SourceID	plos doaj pubmedcentral proquest pubmed crossref
SourceType	Open Website Open Access Repository Aggregation Database Index Database Enrichment Source
StartPage	e7215
SubjectTerms	Animals Antibodies Antibody response Antigens Avidity B-Cell Activation Factor Receptor - metabolism B-cell receptor B-Lymphocytes - virology Binding CD79 Antigens - metabolism Cell activation Cell Membrane - metabolism Epitopes - chemistry Female Glycoprotein gp41 HIV HIV Envelope Protein gp41 - metabolism HIV-1 - metabolism Human immunodeficiency virus Immunoglobulin M Immunoglobulins Immunological tolerance Immunology Immunology/Genetics of the Immune System Immunology/Immune Response Immunology/Immunity to Infections Immunology/Immunomodulation Immunology/Leukocyte Signaling and Gene Expression Lymphocyte Activation Lymphocyte receptors Lymphocytes B Mice Mice, Inbred BALB C Mice, Inbred C57BL Monoclonal antibodies Peptides Peritoneum Protein Binding Receptors Signal Transduction Signaling Spleen Spleen - metabolism Vaccines Virology
SummonAdditionalLinks	– databaseName: Directory of Open Access Journals dbid: DOA link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELZQxYELorwaKMgHDiBhGseJH0eKWLUSVByg6i1ybKestJtdbVp-AL-cGdtZuqhSL9yi2HmM5-EZe_wNIW-cstxaG5iyTc3qxnuwg14x7qURTkFIFI-PnX9RZ2f64sJ8u1HqC3PCEjxwGrij2nsnOO-6Wla1AkvaKQdTii9tbcu-ita3VGYKppINBi2WMh-UE4ofZb58WK-GgIiFEPY0OxNRxOtHfNPFarzN1_w3ZfLGHDR7RB5m55F-TD-9T-6F4THZz-o50rcZQ_rdE_J7BvNVWuZ7TyHCZ26B1_T08qtlWI4DzB_4mvSY4so9BbMX1hB9U0SP2KSzDiPFNVoKDiI9OT1nnIYh5hcFermuOV2GJQTaQ6CYCDNfwrsnQGmKxR5Ww1PyY_b5-6cTlsstMCdU2TDrS-BckGVtZe-17iAWc6HXRrkmOF11EV7NCRjcXnSgzAJ6O9VzY8EJ6bR4RvaAoHBAqAAnEV7X-xKY1nNtvJZaCq_xrKvluiBiGvvWZSxyLImxaOMGm4KYJI1oixxrM8cKwrZPrRMWxx39j5Gt276IpB1vgHy1Wb7au-SrIAcoFNMHxhYcpCrCpNUFOZwE5fZmum0GnUV2AldW12OLEJRGm4oX5HkSq7_0GF1iCYOCqB2B2yFit2WY_4yw4BXuoHH54n9Q_ZI8iNtmmCkhD8ne1eY6vCL33a-r-bh5HXXtD-NiMLk priority: 102 providerName: Directory of Open Access Journals – databaseName: Public Library of Science (PLoS) Journals Open Access dbid: FPL link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3Nb9MwFLdgcOACjK9lDOQDB5AwxLFjO0dWtdqkraoQTLtFju1slbKkajbEeX85z47b0WkT4hbFjuO8j_j3_OyfEfpgpKZaa0ekzjnhubXwH7SSUCsKZiSERGH72MmRnE7V6WkxuwkUb2XwmaRfo0y_LLrWebZBCFnyh-hRxoTwwdZkdrT684LvChG3x9335MbwE1j6Patp0_V3IczbCyX_Gnkmz_63z8_R04gx8bfBKLbRA9e-QNvRi3v8MVJNf3qJricwrA2zgZ_xtGvJqPHX-PDsWJPvzp_pYQCS4n08ck2DAWO6BQTpOMwjDlsieuyncjHgSHxweEIoHrdhGZLDZwtO8bG7gHi8dXi27H7PL6DtceSdhtb8WuhX6Odk_GN0QOKpDMQwmeZE2xQU7ETKtaitUhWEbMbVqpAmd0ZlVWBhMwy0UbMKfJ5BbSNrWmjAKpVir9FWC3LZQZgBloTmapuKjNdUFVYJJZhVfkuspipBbKWs0kTKcn9yRlOGPJyE0GWQaOkFXUZBJ4isn1oMlB3_qL_v7WBd1xNuhxug0TL6b8mtNYzSquLQVQkDeiUNIBubaq7TOssStOOtaPWCvgQclQU2NZ6gvZVl3V2M18Xg2j5fA1rprvrSM1UWqshogt4MdnjzPYVK_UkHCZIbFrrxEZsl7fw8sIdnPtFGxe79HX6LnoScmV8mIfbQ1uXyyr1Dj82vy3m_fB9c7g9BaiwZ priority: 102 providerName: Public Library of Science
Title	Functional, Non-Clonal IgMa-Restricted B Cell Receptor Interactions with the HIV-1 Envelope gp41 Membrane Proximal External Region
URI	https://www.ncbi.nlm.nih.gov/pubmed/19806186 https://www.proquest.com/docview/1292305554 https://www.proquest.com/docview/733698921 https://pubmed.ncbi.nlm.nih.gov/PMC2751816 https://doaj.org/article/4ddc311bb46247718b7c614d0a4a0f22 http://dx.doi.org/10.1371/journal.pone.0007215
Volume	4
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVAON databaseName: DOAJ Open Access Journals customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: DOA dateStart: 20060101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M~E dateStart: 20060101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Advanced Technologies & Aerospace Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: P5Z dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/hightechjournals providerName: ProQuest – providerCode: PRVPQU databaseName: Agricultural Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M0K dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/agriculturejournals providerName: ProQuest – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7P dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Engineering Database (subscription) customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7S dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com providerName: ProQuest – providerCode: PRVPQU databaseName: Environmental Science Database (subscripiton) customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PATMY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/environmentalscience providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7X7 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: Materials Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: KB. dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/materialsscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Nursing & allied health premium. customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7RV dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/nahs providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: BENPR dateStart: 20061201 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Public Health Database (Proquest) customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 8C1 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/publichealth providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PIMPY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVATS databaseName: Public Library of Science (PLoS) Journals Open Access customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: FPL dateStart: 20060101 isFulltext: true titleUrlDefault: http://www.plos.org/publications/ providerName: Public Library of Science
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3Nb9MwFLdg48AFGB9bYFQ-cACJbHGcxM4J0arVqq1VVKAqXCLHdkqlLinNhjjzl_PsuB1FExy4PFW148Z979nvw_49hF5JJogQQvtMxJEfxUrBOqiYT1SSUsnAJbLXx6YXbDzms1mauYBb445VbtZEu1CrWpoY-SnsS6FFp4rerb75pmqUya66Ehp30T4xtjHIM5tMNysx6HKSuOtylJFTx52TVV1pg1sIzk-8sx1Z1H6Dcrqsm9sszj8PTv62Ew0e_u8cHqEHzgbF71uhOUB3dPUYHTgtb_BrB0X95gn6OYBtr40WvsXjuvJ7S_MZD-cj4U-0qfkhwWTFXdzTyyUGG1SvwInHNs7YXplosAn1YrAz8dlw6hPcr-wxJY3nq4jgkb4Ef73SOFvXPxaXMHbf4VLDaOas9FP0adD_2DvzXdUGX1IWxL5QAQiAToJIJKXivACXTuqSp0zGWvKwsChtkgJ3SlrAmkCht2QlSQXYMgWnz9BeBRw6QpiCrQnDlSpIwqgkPFU84QlV3FyZFYR7iG6Yl0sHaW4qayxzm6dj4Nq0_2huWJ47lnvI3z61aiE9_tG_a-Ri29cActsv6vU8d_qdR0pJSkhRRPCqDDb8gkmwfFQgIhGUYeihIyNVmx9o8htZ8NDxRlpub8bbZlB9k88BrtTXTW6QLFOehsRDh61c3swn5YGphOAhtiOxO5PYbakWXy26eGgScSR5_ve3eoHu27yaOUqRHKO9q_W1fonuye9Xi2bdsWpo6IxZyoHyHumg_W5_nE06NvIBdJBdAD3vngAdBeeGsszSD0Cz-As8kQ1H2edf_pdKgg
linkProvider	ProQuest
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V3Nb9MwFLfGQIILML4WGOADSCBhFsdJ7BwQYqXVqrVVhcY0cQmO7ZRKXVKajo8rfxB_I89O0lE0wWkHblHsOI7ze8_v2c-_h9ATxSWVUhrCZRSSMNIa9KDmhOo4YYqDS-SOjx0N-Ggkjo-T8Qb62Z6FsWGVrU50ilqXyq6R78K8FDh2qvD1_DOxWaPs7mqbQqOGxYH5_hVctupV_y3836dB0OsedvZJk1WAKMb9iEjtQwdN7IcyzrUQGbgcyuQi4SoySgSZYxFTDDRBzjLALIPaiuc0kTDXZoJBu5fQ5TAMfCtF4-hDq_nhiThujucxTncbNLycl4WxPIngbEVr05_LEmBZVWdldZ6F-2eg5m8zX-_G_zZmN9H1xsbGb2qh2EIbpriFthotVuFnDdX289voRw-m9Xo19AUelQXpzOw17k-GkrwzNqeJApMc7-GOmc0w2NhmviwX2K2j1kdCKmyXsjHY0Xi_f0Qo7hYuDMvgyTykeGhOMjAHDB4vym_TE2i72_BuQ2s2FvwOen8hY3EXbRaAiG2EGdjS0Fyu_TgIcyoSLWIRMy3skWBJhYdYC5ZUNZTtNnPILHX7kBxct3pEUwuxtIGYh8jqqXlNWfKP-nsWh6u6lnDc3SgXk7TRX2motWKUZlkIXeVg0GRcgWWnfRlKPw8CD21bFLcvqNIz7Hlop0Xn-cV4VQyqze5XwV8pT6vUMnUmIgmoh-7VcnD2PYnwbaYHD_E1CVn7iPWSYvrJsacHdqORxvf_3qvH6Or-4XCQDvqjgwfomttDtGEj8Q7aXC5OzUN0RX1ZTqvFI6cCMPp40fLzC1Mqml8
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Nb9MwGLbGQGgXYHxsgQE-gAQSoXGcxM4BIda1WrWtqhBME5fg2E6p1CWl6fi48rP4dbx2nI6iCU47cItix3Gcx--H_fp5EXoimSBCCO0zEUd-FCsFclAxn6gkpZKBS2SPjx0fsuGQn5ykozX0sz0LY8IqW5loBbWqpFkj74BeCi07VdQpXFjEaK__evbZNxmkzE5rm06jgciB_v4V3Lf61WAP_vXTMOz33nX3fZdhwJeUBbEvVACd1UkQiaRQnOfgfkhd8JTJWEse5pZRTFKQCgXNAb8UaktWkFSA3s05hXavoKsMfEwTTjiKP7RaAJ5IEndUjzLScch4OatKbTgTwfGKV1ShzRhgGFanVX2Rtftn0OZvWrB_838ev1vohrO98ZtmsmyiNV3eRptOutX4maPgfn4H_eiDum9WSV_gYVX63am5xoPxkfDfapPrRIKpjndxV0-nGGxvPVtUc2zXV5ujIjU2S9wY7Gu8Pzj2Ce6VNjxL4_EsIvhIn-ZgJmg8mlffJqfQds_xcUNrJkb8Lnp_KWNxD62XgI5thCnY2NBcoYIkjArCU8UTnlDFzVFhQbiHaAucTDoqd5NRZJrZ_UkGLl0zopmBW-bg5iF_-dSsoTL5R_1dg8llXUNEbm9U83Hm5FoWKSUpIXkeQVcZGDo5k2DxqUBEIijC0EPbBtHtC-rsHIce2mmRenExXhaDyDP7WPBXqrM6MwyeKU9D4qGtZk6cf0_KA5MBwkNsZbasfMRqSTn5ZFnVQ7MBSZL7f-_VY3Qdpk12OBgePEAbdmvRRJMkO2h9MT_TD9E1-WUxqeePrDTA6ONlT59fsw2jKQ
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Functional%2C+Non-Clonal+IgMa-Restricted+B+Cell+Receptor+Interactions+with+the+HIV-1+Envelope+gp41+Membrane+Proximal+External+Region&rft.jtitle=PloS+one&rft.au=Verkoczy%2C+Laurent&rft.au=Moody%2C+M.+Anthony&rft.au=Holl%2C+T.+Matt&rft.au=Bouton-Verville%2C+Hilary&rft.date=2009-10-06&rft.issn=1932-6203&rft.eissn=1932-6203&rft.volume=4&rft.issue=10&rft.spage=e7215&rft_id=info:doi/10.1371%2Fjournal.pone.0007215&rft.externalDBID=n%2Fa&rft.externalDocID=10_1371_journal_pone_0007215
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon