Molecular cloning of the bovine prolactin receptor and distribution of prolactin and growth hormone receptor transcripts in fetal and utero-placental tissues

We have isolated a bovine prolactin (bPRL) receptor cDNA from an endometrial cDNA library, which predicts a 557 amino acid transmembrane protein similar to the long forms of other characterized prolactin receptors. The predicted cytoplasmic domain is slightly truncated primarily by a stop codon loca...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Molecular and cellular endocrinology Ročník 89; číslo 1-2; s. 47
Hlavní autoři: Scott, P, Kessler, M A, Schuler, L A
Médium: Journal Article
Jazyk:angličtina
Vydáno: Ireland 01.11.1992
Témata:
Amino Acid Sequence
Animals
Base Sequence
Cattle - embryology
Cattle - genetics
Cattle - metabolism
Cell Line
Cercopithecus aethiops
Cloning, Molecular
DNA - genetics
Female
Fetus - chemistry
Molecular Sequence Data
Multigene Family
Organ Specificity
Placenta - chemistry
Placental Lactogen - metabolism
Polymerase Chain Reaction
Pregnancy
Pregnancy Proteins - analysis
Pregnancy, Animal - metabolism
Prolactin - metabolism
Receptors, Cell Surface - genetics
Receptors, Peptide
Receptors, Prolactin - analysis
Receptors, Prolactin - genetics
Receptors, Somatotropin - analysis
Recombinant Fusion Proteins - biosynthesis
Transcription, Genetic
Uterus - chemistry
ISSN:0303-7207
On-line přístup:Zjistit podrobnosti o přístupu
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:We have isolated a bovine prolactin (bPRL) receptor cDNA from an endometrial cDNA library, which predicts a 557 amino acid transmembrane protein similar to the long forms of other characterized prolactin receptors. The predicted cytoplasmic domain is slightly truncated primarily by a stop codon located 36 codons 5' from the stop utilized in the human hepatic transcript. When expressed in COS cells, this cDNA was shown to encode a protein which bound bovine placental lactogen (bPL) and bPRL with nearly equal affinity (KD for bPL, 2.03 x 10(-10) M; bPRL, 3.07 x 10(-10) M). Northern analysis demonstrated multiple transcripts, with maternal liver, corpus luteum, intestine, endometrium and fetal liver containing a major transcript of about 3.8 kb, and maternal corpus luteum and endometrium, a second sized transcript of apparently equal abundance of 4.4 kb. This difference did not appear to be within the coding region. Primer extension analysis of maternal hepatic and endometrial transcripts revealed considerable heterogeneity. Examination of the distribution of prolactin and growth hormone receptor transcripts at mid-pregnancy by semi-quantitative reverse transcriptase polymerase chain reaction showed that both are widespread in bovine fetal and placental tissues. This isolation of bovine prolactin receptor cDNA, and description of receptor distribution will facilitate study of the action of the placental and pituitary members of this gene family during pregnancy.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0303-7207
DOI:10.1016/0303-7207(92)90210-W