Generation of human oogonia from induced pluripotent stem cells in vitro

Human in vitro gametogenesis may transform reproductive medicine. Human pluripotent stem cells (hPSCs) have been induced into primordial germ cell-like cells (hPGCLCs); however, further differentiation to a mature germ cell has not been achieved. Here, we show that hPGCLCs differentiate progressivel...

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Published in:Science (American Association for the Advancement of Science) Vol. 362; no. 6412; p. 356
Main Authors: Yamashiro, Chika, Sasaki, Kotaro, Yabuta, Yukihiro, Kojima, Yoji, Nakamura, Tomonori, Okamoto, Ikuhiro, Yokobayashi, Shihori, Murase, Yusuke, Ishikura, Yukiko, Shirane, Kenjiro, Sasaki, Hiroyuki, Yamamoto, Takuya, Saitou, Mitinori
Format: Journal Article
Language:English
Published: United States 19.10.2018
Subjects:
Cellular Reprogramming Techniques - methods
DNA Methylation
Epigenesis, Genetic
Female
Humans
Induced Pluripotent Stem Cells - cytology
Oogenesis
Oogonia - cytology
Ovary - growth & development
ISSN:1095-9203, 1095-9203
Online Access:Get more information
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Summary:Human in vitro gametogenesis may transform reproductive medicine. Human pluripotent stem cells (hPSCs) have been induced into primordial germ cell-like cells (hPGCLCs); however, further differentiation to a mature germ cell has not been achieved. Here, we show that hPGCLCs differentiate progressively into oogonia-like cells during a long-term in vitro culture (approximately 4 months) in xenogeneic reconstituted ovaries with mouse embryonic ovarian somatic cells. The hPGCLC-derived oogonia display hallmarks of epigenetic reprogramming-genome-wide DNA demethylation, imprint erasure, and extinguishment of aberrant DNA methylation in hPSCs-and acquire an immediate precursory state for meiotic recombination. Furthermore, the inactive X chromosome shows a progressive demethylation and reactivation, albeit partially. These findings establish the germline competence of hPSCs and provide a critical step toward human in vitro gametogenesis.
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ISSN:1095-9203
1095-9203
DOI:10.1126/science.aat1674