Aptamer-Protein Interactions: From Regulation to Biomolecular Detection
Serving as the basis of cell life, interactions between nucleic acids and proteins play essential roles in fundamental cellular processes. Aptamers are unique single-stranded oligonucleotides generated by in vitro evolution methods, possessing the ability to interact with proteins specifically. Alte...
Saved in:
| Published in: | Chemical reviews Vol. 123; no. 22; p. 12471 |
|---|---|
| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
22.11.2023
|
| Subjects: | |
| ISSN: | 1520-6890, 1520-6890 |
| Online Access: | Get more information |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Serving as the basis of cell life, interactions between nucleic acids and proteins play essential roles in fundamental cellular processes. Aptamers are unique single-stranded oligonucleotides generated by in vitro evolution methods, possessing the ability to interact with proteins specifically. Altering the structure of aptamers will largely modulate their interactions with proteins and further affect related cellular behaviors. Recently, with the in-depth research of aptamer-protein interactions, the analytical assays based on their interactions have been widely developed and become a powerful tool for biomolecular detection. There are some insightful reviews on aptamers applied in protein detection, while few systematic discussions are from the perspective of regulating aptamer-protein interactions. Herein, we comprehensively introduce the methods for regulating aptamer-protein interactions and elaborate on the detection techniques for analyzing aptamer-protein interactions. Additionally, this review provides a broad summary of analytical assays based on the regulation of aptamer-protein interactions for detecting biomolecules. Finally, we present our perspectives regarding the opportunities and challenges of analytical assays for biological analysis, aiming to provide guidance for disease mechanism research and drug discovery. |
|---|---|
| AbstractList | Serving as the basis of cell life, interactions between nucleic acids and proteins play essential roles in fundamental cellular processes. Aptamers are unique single-stranded oligonucleotides generated by in vitro evolution methods, possessing the ability to interact with proteins specifically. Altering the structure of aptamers will largely modulate their interactions with proteins and further affect related cellular behaviors. Recently, with the in-depth research of aptamer-protein interactions, the analytical assays based on their interactions have been widely developed and become a powerful tool for biomolecular detection. There are some insightful reviews on aptamers applied in protein detection, while few systematic discussions are from the perspective of regulating aptamer-protein interactions. Herein, we comprehensively introduce the methods for regulating aptamer-protein interactions and elaborate on the detection techniques for analyzing aptamer-protein interactions. Additionally, this review provides a broad summary of analytical assays based on the regulation of aptamer-protein interactions for detecting biomolecules. Finally, we present our perspectives regarding the opportunities and challenges of analytical assays for biological analysis, aiming to provide guidance for disease mechanism research and drug discovery. Serving as the basis of cell life, interactions between nucleic acids and proteins play essential roles in fundamental cellular processes. Aptamers are unique single-stranded oligonucleotides generated by in vitro evolution methods, possessing the ability to interact with proteins specifically. Altering the structure of aptamers will largely modulate their interactions with proteins and further affect related cellular behaviors. Recently, with the in-depth research of aptamer-protein interactions, the analytical assays based on their interactions have been widely developed and become a powerful tool for biomolecular detection. There are some insightful reviews on aptamers applied in protein detection, while few systematic discussions are from the perspective of regulating aptamer-protein interactions. Herein, we comprehensively introduce the methods for regulating aptamer-protein interactions and elaborate on the detection techniques for analyzing aptamer-protein interactions. Additionally, this review provides a broad summary of analytical assays based on the regulation of aptamer-protein interactions for detecting biomolecules. Finally, we present our perspectives regarding the opportunities and challenges of analytical assays for biological analysis, aiming to provide guidance for disease mechanism research and drug discovery.Serving as the basis of cell life, interactions between nucleic acids and proteins play essential roles in fundamental cellular processes. Aptamers are unique single-stranded oligonucleotides generated by in vitro evolution methods, possessing the ability to interact with proteins specifically. Altering the structure of aptamers will largely modulate their interactions with proteins and further affect related cellular behaviors. Recently, with the in-depth research of aptamer-protein interactions, the analytical assays based on their interactions have been widely developed and become a powerful tool for biomolecular detection. There are some insightful reviews on aptamers applied in protein detection, while few systematic discussions are from the perspective of regulating aptamer-protein interactions. Herein, we comprehensively introduce the methods for regulating aptamer-protein interactions and elaborate on the detection techniques for analyzing aptamer-protein interactions. Additionally, this review provides a broad summary of analytical assays based on the regulation of aptamer-protein interactions for detecting biomolecules. Finally, we present our perspectives regarding the opportunities and challenges of analytical assays for biological analysis, aiming to provide guidance for disease mechanism research and drug discovery. |
| Author | Wei, Junyuan Yuan, Quan Hou, Xinru Ji, Cailing Zhang, Lei Tan, Jie Tan, Weihong |
| Author_xml | – sequence: 1 givenname: Cailing surname: Ji fullname: Ji, Cailing organization: Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China – sequence: 2 givenname: Junyuan surname: Wei fullname: Wei, Junyuan organization: Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China – sequence: 3 givenname: Lei surname: Zhang fullname: Zhang, Lei organization: Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China – sequence: 4 givenname: Xinru surname: Hou fullname: Hou, Xinru organization: Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China – sequence: 5 givenname: Jie orcidid: 0000-0002-0909-2904 surname: Tan fullname: Tan, Jie organization: Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China – sequence: 6 givenname: Quan orcidid: 0000-0002-3085-431X surname: Yuan fullname: Yuan, Quan organization: Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China – sequence: 7 givenname: Weihong orcidid: 0000-0002-8066-1524 surname: Tan fullname: Tan, Weihong organization: The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/37931070$$D View this record in MEDLINE/PubMed |
| BookMark | eNpNj81Kw0AYRQep2B99AkGydJM6mf-4q1VroaCIrsPkyxeNJJk6MxF8e6tWcHUvh8OFOyWj3vVIyGlG5xll2YWFMIdX7Dx-zDlQyrU-IJNMMpoqk9PRvz4m0xDeKKVSMn1ExlznPKOaTshqsY22Q58-eBex6ZN1H9FbiI3rw2Vy612XPOLL0NpvkkSXXDWucy3CDvnkGiP-uMfksLZtwJN9zsjz7c3T8i7d3K_Wy8UmBa5UTEHXQjMrJLASSsUEo7w0plIVMtAaoEaweS51rYRSSgttuKisoVJYIytgM3L-u7v17n3AEIuuCYBta3t0QyiYMSrnWqh8p57t1aHssCq2vums_yz-zrMv_K5gHQ |
| CitedBy_id | crossref_primary_10_1021_acs_analchem_5c03351 crossref_primary_10_1021_jacs_4c03129 crossref_primary_10_1360_CSB_2025_0510 crossref_primary_10_1016_j_foodcont_2025_111300 crossref_primary_10_1016_j_ijbiomac_2024_133935 crossref_primary_10_1016_j_ymthe_2025_02_047 crossref_primary_10_1021_acsanm_4c07300 crossref_primary_10_1002_chem_202501034 crossref_primary_10_1016_j_jfca_2025_107902 crossref_primary_10_1016_j_jpba_2025_117055 crossref_primary_10_1002_smtd_202401835 crossref_primary_10_1021_acs_analchem_4c05482 crossref_primary_10_1016_j_microc_2025_113979 crossref_primary_10_1142_S0219720025500106 crossref_primary_10_1016_j_cej_2025_161838 crossref_primary_10_1016_j_microc_2024_111995 crossref_primary_10_1016_j_trac_2025_118349 crossref_primary_10_1080_10408347_2023_2298328 crossref_primary_10_1016_j_snb_2024_135529 crossref_primary_10_1016_j_sbi_2024_102846 crossref_primary_10_1002_aelm_202500010 crossref_primary_10_1039_D4SC08004A crossref_primary_10_1016_j_talanta_2025_128370 crossref_primary_10_1021_acs_nanolett_5c02356 crossref_primary_10_1002_mco2_70180 crossref_primary_10_1016_j_bios_2024_116998 crossref_primary_10_1016_j_bios_2025_117704 crossref_primary_10_1016_j_lmd_2024_100011 crossref_primary_10_1038_s41551_025_01503_8 crossref_primary_10_1080_17576180_2024_2368340 crossref_primary_10_1002_adfm_202425035 crossref_primary_10_1016_j_talanta_2025_127809 crossref_primary_10_3390_bios15090629 crossref_primary_10_3390_molecules29133013 crossref_primary_10_1021_acsnano_5c12854 crossref_primary_10_1186_s12951_025_03627_0 crossref_primary_10_1002_cbic_202400599 crossref_primary_10_1016_j_jelechem_2024_118194 crossref_primary_10_1016_j_ijbiomac_2024_138265 crossref_primary_10_1038_s41570_025_00721_7 crossref_primary_10_1039_D4NH00652F crossref_primary_10_3390_ijms25168888 crossref_primary_10_1016_j_actbio_2024_10_022 crossref_primary_10_1016_j_talanta_2024_127199 crossref_primary_10_1002_advs_202410642 crossref_primary_10_1016_j_cej_2025_163703 crossref_primary_10_1007_s44211_025_00828_3 crossref_primary_10_1002_cac2_12518 crossref_primary_10_1016_j_ijbiomac_2025_144318 crossref_primary_10_1016_j_ijbiomac_2025_145968 crossref_primary_10_1186_s12870_025_06896_x crossref_primary_10_1007_s00216_025_06091_9 crossref_primary_10_1021_acs_molpharmaceut_5c00352 crossref_primary_10_1002_mabi_202400327 crossref_primary_10_1016_j_talanta_2024_126143 crossref_primary_10_1016_j_biotechadv_2024_108491 crossref_primary_10_1002_anie_202503818 crossref_primary_10_1016_j_jpha_2025_101369 crossref_primary_10_3390_ijms242216318 crossref_primary_10_1016_j_ijbiomac_2024_138099 crossref_primary_10_3390_molecules29102215 crossref_primary_10_1002_advs_202500433 crossref_primary_10_1080_1061186X_2024_2373306 crossref_primary_10_1021_acs_analchem_5c01627 crossref_primary_10_1016_j_cej_2025_163557 crossref_primary_10_1038_s41392_024_02112_8 crossref_primary_10_1063_1674_0068_cjcp2312146 crossref_primary_10_1038_s41467_025_63040_x crossref_primary_10_1016_j_chroma_2024_465141 crossref_primary_10_1002_advs_202511099 crossref_primary_10_1002_ange_202503818 crossref_primary_10_1016_j_eurpolymj_2024_113708 crossref_primary_10_1016_j_cej_2024_158630 crossref_primary_10_1016_j_saa_2024_125056 crossref_primary_10_1016_j_ccr_2024_216245 crossref_primary_10_1021_acs_analchem_4c03307 crossref_primary_10_1021_acs_analchem_5c01915 crossref_primary_10_1016_j_snb_2025_138384 crossref_primary_10_1021_jacs_4c06189 crossref_primary_10_1002_cjoc_70094 crossref_primary_10_1039_D4QM00731J crossref_primary_10_1021_acs_analchem_5c03778 crossref_primary_10_1038_s42004_025_01629_5 crossref_primary_10_1016_j_talanta_2024_126044 crossref_primary_10_1016_j_ejps_2024_106793 crossref_primary_10_1016_j_trac_2025_118354 |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1021/acs.chemrev.3c00377 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Chemistry |
| EISSN | 1520-6890 |
| ExternalDocumentID | 37931070 |
| Genre | Research Support, Non-U.S. Gov't Journal Article Review |
| GroupedDBID | --- -DZ -~X .DC .K2 29B 4.4 53G 55A 5GY 5RE 5VS 6J9 7~N 85S AABXI AAHBH ABJNI ABMVS ABPPZ ABQRX ABUCX ACGFO ACGFS ACGOD ACIWK ACJ ACNCT ACS ADHLV AEESW AENEX AFEFF AFXLT AGXLV AHGAQ ALMA_UNASSIGNED_HOLDINGS AQSVZ BAANH BKOMP CGR CS3 CUPRZ CUY CVF D0L DU5 EBS ECM ED~ EIF F5P GGK GNL IH9 IHE JG~ LG6 NPM P2P PQQKQ ROL RWL TAE TN5 UI2 UKR UPT VF5 VG9 VQA W1F WH7 XSW YZZ ~02 7X8 ABBLG ABLBI |
| ID | FETCH-LOGICAL-c366t-c7f472a45c2bcb624203b88d6de2c77ccfeca9957f64666747834da8054a85dc2 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 106 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=001108422200001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1520-6890 |
| IngestDate | Fri Jul 11 16:46:22 EDT 2025 Thu Jan 02 22:40:32 EST 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 22 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c366t-c7f472a45c2bcb624203b88d6de2c77ccfeca9957f64666747834da8054a85dc2 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 ObjectType-Review-3 content type line 23 |
| ORCID | 0000-0002-0909-2904 0000-0002-3085-431X 0000-0002-8066-1524 |
| PMID | 37931070 |
| PQID | 2886937469 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_2886937469 pubmed_primary_37931070 |
| PublicationCentury | 2000 |
| PublicationDate | 2023-11-22 |
| PublicationDateYYYYMMDD | 2023-11-22 |
| PublicationDate_xml | – month: 11 year: 2023 text: 2023-11-22 day: 22 |
| PublicationDecade | 2020 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Chemical reviews |
| PublicationTitleAlternate | Chem Rev |
| PublicationYear | 2023 |
| SSID | ssj0005527 |
| Score | 2.6796865 |
| SecondaryResourceType | review_article |
| Snippet | Serving as the basis of cell life, interactions between nucleic acids and proteins play essential roles in fundamental cellular processes. Aptamers are unique... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 12471 |
| SubjectTerms | Aptamers, Nucleotide - chemistry Aptamers, Nucleotide - metabolism Nucleic Acids Proteins SELEX Aptamer Technique - methods |
| Title | Aptamer-Protein Interactions: From Regulation to Biomolecular Detection |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/37931070 https://www.proquest.com/docview/2886937469 |
| Volume | 123 |
| WOSCitedRecordID | wos001108422200001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LS8QwEA7qCnrx_VhfRPBatpukSepFdHX1oMsiCntbkjTBPbRdt9Xf76QP9iQIXnooFNrpZOabSeb7ELpyhoZO9K2fzOUBMzYKNPOqqdznz0QDQq9US57FaCQnk3jcNNyK5lhlGxOrQJ3kxvfIe0RKDqkUqrmb-WfgVaP87mojobGKOhSgjPdqMVmyhUe1ZCukKCiRZBy2rEOk31MGfumHTb3QCzWehUX8jjGrXDPc_u9b7qCtBmXi29otdtGKzfbQxqAVd9tHj7fzUqV2EYw9T8Msw1VnsB5yKK7xcJGn-LWWqYc7uMzx3SxPWyldfG_L6ghXdoDehw9vg6eg0VQIDOW8DIxwTBDFIkO00X44JKRayoQnlhghjHHWqDiOhOMMKhvPrk9ZoiQgOyWjxJBDtJblmT1GWCexBjgmWBI65jRRkdIx49orVEEVQrvosrXRFL7Ob0SozOZfxXRppS46qg09ndfkGlMKAQNK0vDkD0-fok2v_u5HAwk5Qx0HK9aeo3XzXc6KxUXlDHAdjV9-AAHOv60 |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Aptamer-Protein+Interactions%3A+From+Regulation+to+Biomolecular+Detection&rft.jtitle=Chemical+reviews&rft.au=Ji%2C+Cailing&rft.au=Wei%2C+Junyuan&rft.au=Zhang%2C+Lei&rft.au=Hou%2C+Xinru&rft.date=2023-11-22&rft.eissn=1520-6890&rft.volume=123&rft.issue=22&rft.spage=12471&rft_id=info:doi/10.1021%2Facs.chemrev.3c00377&rft_id=info%3Apmid%2F37931070&rft_id=info%3Apmid%2F37931070&rft.externalDocID=37931070 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1520-6890&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1520-6890&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1520-6890&client=summon |