Design and characterization of multifaceted lyophilized liposomal wafers with promising wound healing potential

Various dressings are available to heal chronic wounds which many times fail to achieve the expected results. To overcome some of their drawbacks, formulation of a novel dressing; lyophilized liposomal wafers having better wound healing potential has been proposed in the present study. The drug inco...

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Bibliographic Details
Published in:Journal of liposome research Vol. 28; no. 3; pp. 193 - 208
Main Authors: Avachat, Amelia M., Takudage, Pooja J.
Format: Journal Article
Language:English
Published: England Taylor & Francis 03.07.2018
Subjects:
Animals
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Chitosan
Chitosan - chemistry
Cholesterol - chemistry
chronic wound
Drug Liberation
Female
Freeze Drying
gatifloxacin (GTX)
Gatifloxacin - chemistry
Gatifloxacin - pharmacology
Lipids - chemistry
liposomal gel
Liposomes - chemistry
lyophilized liposomal wafers
Male
Mechanical Phenomena
Particle Size
Rats, Wistar
Surface Properties
Viscosity
Wound Healing - drug effects
gatifloxacin (GTX)
Chitosan
lyophilized liposomal wafers
chronic wound
liposomal gel
ISSN:0898-2104, 1532-2394, 1532-2394
Online Access:Get full text
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Summary:Various dressings are available to heal chronic wounds which many times fail to achieve the expected results. To overcome some of their drawbacks, formulation of a novel dressing; lyophilized liposomal wafers having better wound healing potential has been proposed in the present study. The drug incorporated in the formulation is gatifloxacin (GTX) which is a fourth-generation fluoroquinolone antibiotic having in vitro activity against both Gram-negative and Gram-positive bacteria. The formulation was designed in three stages where at first liposomes were prepared, the liposomes were converted to gel using chitosan and lastly this gel was lyophilized to form liposomal wafers. Liposomes were prepared by varying the concentration of lipid and cholesterol and evaluated for particle size, entrapment efficiency, in vitro cumulative release, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Liposomes were converted to liposomal gel using chitosan and evaluated for texture, clarity, viscosity, spreadibility and in vitro drug release. Finally, this liposomal batch was subjected to lyophilization to convert it to liposomal wafers and subjected to SEM, differential scanning calorimetric, X-ray diffraction and drug release studies. The in vivo studies were carried out on Wistar rats where wound healing potential of the wafers was confirmed by histopathological evaluation.
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ISSN:0898-2104
1532-2394
1532-2394
DOI:10.1080/08982104.2017.1335319