Kinetic analysis of pulmonary neutrophil retention in vivo using the multiple-indicator-dilution technique

Multiple-indicator-dilution experiments were performed in the lungs of 13 anesthetized dogs by simultaneous bolus injection of 111In-labeled neutrophils, 51Cr-labeled red blood cells, and Evans blue-labeled albumin. Concomitant counts of unlabeled neutrophils were similar in pulmonary artery and aor...

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Veröffentlicht in:Journal of applied physiology (1985) Jg. 95; H. 1; S. 279
Hauptverfasser: Schwab, Andreas J, Salamand, Agnes, Merhi, Yahye, Simard, Andre, Dupuis, Jocelyn
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.07.2003
Schlagworte:
Albumins - metabolism
Algorithms
Animals
Dogs
Dye Dilution Technique
Hemodynamics - physiology
Indium Radioisotopes
Kinetics
Leukocyte Count
Lung - cytology
Models, Biological
Models, Statistical
Neutrophils - physiology
Pulmonary Circulation - physiology
Pulmonary Gas Exchange - physiology
ISSN:8750-7587
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Zusammenfassung:Multiple-indicator-dilution experiments were performed in the lungs of 13 anesthetized dogs by simultaneous bolus injection of 111In-labeled neutrophils, 51Cr-labeled red blood cells, and Evans blue-labeled albumin. Concomitant counts of unlabeled neutrophils were similar in pulmonary artery and aortic blood samples, demonstrating a dynamic balance across the lungs in the physiological state. Outflow profiles of labeled neutrophils were analyzed on the basis of a recirculatory pharmacokinetic model of labeled albumin. The outflow profiles of the recovered neutrophils were composed of a throughput component of circulating neutrophils and a component of reversibly marginated neutrophils. They were interpreted by a model incorporating neutrophil margination (transfer coefficient = 0.195 +/- 0.081 s-1), rapid demargination (0.054 +/- 0.027 s-1), and transfer to a slow marginated pool (0.023 +/- 0.018 s-1). It will be interesting to apply the analysis in future studies aimed at determining whether it could be a useful research tool to investigate the interactions between the pulmonary endothelium and neutrophils in physiological and diseased states.
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ISSN:8750-7587
DOI:10.1152/japplphysiol.00783.2001