Oral or parenteral administration of replication-deficient adenoviruses expressing the measles virus haemagglutinin and fusion proteins: protective immune responses in rodents

The genes encoding the measles virus (MV) haemagglutinin (H) and fusion (F) proteins were placed under the control of the human cytomegalovirus immediate early promoter in a replication-deficient adenovirus vector. Immunofluorescence and radioimmune precipitation demonstrated the synthesis of each p...

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Bibliographic Details
Published in:Journal of general virology Vol. 79 ( Pt 5); p. 1027
Main Authors: Fooks, A R, Jeevarajah, D, Lee, J, Warnes, A, Niewiesk, S, ter Meulen, V, Stephenson, J R, Clegg, J C
Format: Journal Article
Language:English
Published: England 01.05.1998
Subjects:
Adenoviridae - physiology
Administration, Oral
Animals
Antibodies, Viral - immunology
Cell Line
Cell Line, Transformed
Defective Viruses - physiology
Female
Genetic Vectors
Hemagglutinins, Viral - genetics
Hemagglutinins, Viral - immunology
Humans
Injections, Intraperitoneal
Measles - prevention & control
Measles Vaccine - immunology
Measles virus - immunology
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Rats
Sigmodontinae
Viral Fusion Proteins - genetics
Viral Fusion Proteins - immunology
Virus Replication
ISSN:0022-1317
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Summary:The genes encoding the measles virus (MV) haemagglutinin (H) and fusion (F) proteins were placed under the control of the human cytomegalovirus immediate early promoter in a replication-deficient adenovirus vector. Immunofluorescence and radioimmune precipitation demonstrated the synthesis of each protein and biological activity was confirmed by the detection of haemadsorption and fusion activities in infected cells. Oral as well as parenteral administration of the H-expressing recombinant adenovirus elicited a significant protective response in mice challenged with MV. While the F-expressing adenovirus failed to protect mice, cotton rats immunized with either the H- or F-expressing recombinant showed reduced MV replication in the lungs. Antibodies elicited in mice following immunization with either recombinant had no in vitro neutralizing activity, suggesting a protective mechanism involving a cell-mediated immune response. This study demonstrates the feasibility of using oral administration of adenovirus recombinants to induce protective responses to heterologous proteins.
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ISSN:0022-1317
DOI:10.1099/0022-1317-79-5-1027