Whole-brain mapping of effective connectivity by fMRI with cortex-wide patterned optogenetics

Functional magnetic resonance imaging (fMRI) with optogenetic neural manipulation is a powerful tool that enables brain-wide mapping of effective functional networks. To achieve flexible manipulation of neural excitation throughout the mouse cortex, we incorporated spatiotemporal programmable optoge...

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Veröffentlicht in:Neuron (Cambridge, Mass.) Jg. 111; H. 11; S. 1732
Hauptverfasser: Kim, Seonghoon, Moon, Hyun Seok, Vo, Thanh Tan, Kim, Chang-Ho, Im, Geun Ho, Lee, Sungho, Choi, Myunghwan, Kim, Seong-Gi
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 07.06.2023
Schlagworte:
fMRI
patterned optogenetics
functional connectivity
cerebral blood volume
effective connectivity
optogenetics
fiber bundle
optogenetic fMRI
digital micromirror device
anesthesia
ISSN:1097-4199, 1097-4199
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Zusammenfassung:Functional magnetic resonance imaging (fMRI) with optogenetic neural manipulation is a powerful tool that enables brain-wide mapping of effective functional networks. To achieve flexible manipulation of neural excitation throughout the mouse cortex, we incorporated spatiotemporal programmable optogenetic stimuli generated by a digital micromirror device into an MRI scanner via an optical fiber bundle. This approach offered versatility in space and time in planning the photostimulation pattern, combined with in situ optical imaging and cell-type-specific or circuit-specific genetic targeting in individual mice. Brain-wide effective connectivity obtained by fMRI with optogenetic stimulation of atlas-based cortical regions is generally congruent with anatomically defined axonal tracing data but is affected by the types of anesthetics that act selectively on specific connections. fMRI combined with flexible optogenetics opens a new path to investigate dynamic changes in functional brain states in the same animal through high-throughput brain-wide effective connectivity mapping.
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ISSN:1097-4199
1097-4199
DOI:10.1016/j.neuron.2023.03.002