SHIELD: Skull-shaped hemispheric implants enabling large-scale electrophysiology datasets in the mouse brain
To understand the neural basis of behavior, it is essential to measure spiking dynamics across many interacting brain regions. Although new technologies, such as Neuropixels probes, facilitate multi-regional recordings, significant surgical and procedural hurdles remain for these experiments to achi...
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| Vydáno v: | Neuron (Cambridge, Mass.) Ročník 112; číslo 17; s. 2869 |
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| Hlavní autoři: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
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United States
04.09.2024
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| ISSN: | 1097-4199, 1097-4199 |
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| Abstract | To understand the neural basis of behavior, it is essential to measure spiking dynamics across many interacting brain regions. Although new technologies, such as Neuropixels probes, facilitate multi-regional recordings, significant surgical and procedural hurdles remain for these experiments to achieve their full potential. Here, we describe skull-shaped hemispheric implants enabling large-scale electrophysiology datasets (SHIELD). These 3D-printed skull-replacement implants feature customizable insertion holes, allowing dozens of cortical and subcortical structures to be recorded in a single mouse using repeated multi-probe insertions over many days. We demonstrate the procedure's high success rate, biocompatibility, lack of adverse effects on behavior, and compatibility with imaging and optogenetics. To showcase SHIELD's scientific utility, we use multi-probe recordings to reveal novel insights into how alpha rhythms organize spiking activity across visual and sensorimotor networks. Overall, this method enables powerful, large-scale electrophysiological experiments for the study of distributed neural computation. |
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| AbstractList | To understand the neural basis of behavior, it is essential to measure spiking dynamics across many interacting brain regions. Although new technologies, such as Neuropixels probes, facilitate multi-regional recordings, significant surgical and procedural hurdles remain for these experiments to achieve their full potential. Here, we describe skull-shaped hemispheric implants enabling large-scale electrophysiology datasets (SHIELD). These 3D-printed skull-replacement implants feature customizable insertion holes, allowing dozens of cortical and subcortical structures to be recorded in a single mouse using repeated multi-probe insertions over many days. We demonstrate the procedure's high success rate, biocompatibility, lack of adverse effects on behavior, and compatibility with imaging and optogenetics. To showcase SHIELD's scientific utility, we use multi-probe recordings to reveal novel insights into how alpha rhythms organize spiking activity across visual and sensorimotor networks. Overall, this method enables powerful, large-scale electrophysiological experiments for the study of distributed neural computation. To understand the neural basis of behavior, it is essential to measure spiking dynamics across many interacting brain regions. Although new technologies, such as Neuropixels probes, facilitate multi-regional recordings, significant surgical and procedural hurdles remain for these experiments to achieve their full potential. Here, we describe skull-shaped hemispheric implants enabling large-scale electrophysiology datasets (SHIELD). These 3D-printed skull-replacement implants feature customizable insertion holes, allowing dozens of cortical and subcortical structures to be recorded in a single mouse using repeated multi-probe insertions over many days. We demonstrate the procedure's high success rate, biocompatibility, lack of adverse effects on behavior, and compatibility with imaging and optogenetics. To showcase SHIELD's scientific utility, we use multi-probe recordings to reveal novel insights into how alpha rhythms organize spiking activity across visual and sensorimotor networks. Overall, this method enables powerful, large-scale electrophysiological experiments for the study of distributed neural computation.To understand the neural basis of behavior, it is essential to measure spiking dynamics across many interacting brain regions. Although new technologies, such as Neuropixels probes, facilitate multi-regional recordings, significant surgical and procedural hurdles remain for these experiments to achieve their full potential. Here, we describe skull-shaped hemispheric implants enabling large-scale electrophysiology datasets (SHIELD). These 3D-printed skull-replacement implants feature customizable insertion holes, allowing dozens of cortical and subcortical structures to be recorded in a single mouse using repeated multi-probe insertions over many days. We demonstrate the procedure's high success rate, biocompatibility, lack of adverse effects on behavior, and compatibility with imaging and optogenetics. To showcase SHIELD's scientific utility, we use multi-probe recordings to reveal novel insights into how alpha rhythms organize spiking activity across visual and sensorimotor networks. Overall, this method enables powerful, large-scale electrophysiological experiments for the study of distributed neural computation. |
| Author | Gillis, Ryan Siegle, Joshua H Farrell, Colin Williford, Allison Ouellette, Ben Belski, Hannah Lynch, Galen F Sridhar, Arjun Ha, Vivian Ramirez, Tamina K Grasso, Conor Groblewski, Peter A Browning, Yoni Cabasco, Hannah Loeffler, Henry Mochizuki, Chris Suarez, Lucas Bennett, Corbett Caldejon, Shiella Olsen, Shawn R Durand, Severine Guthrie, Alan McBride, Ethan G Johnson, Tye Smith, Heston Wilkes, Joshua Hardcastle, Ben Gale, Samuel Sullivan, David Howard, Robert Han, Warren Cahoon, Alex Lakunina, Anna Swapp, Jackie |
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Electronic address: corbettb@alleninstitute.org – sequence: 2 givenname: Ben surname: Ouellette fullname: Ouellette, Ben organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 3 givenname: Tamina K surname: Ramirez fullname: Ramirez, Tamina K organization: Allen Institute, Seattle, WA 98109, USA – sequence: 4 givenname: Alex surname: Cahoon fullname: Cahoon, Alex organization: Allen Institute, Seattle, WA 98109, USA – sequence: 5 givenname: Hannah surname: Cabasco fullname: Cabasco, Hannah organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 6 givenname: Yoni surname: Browning fullname: Browning, Yoni organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 7 givenname: Anna surname: Lakunina fullname: Lakunina, Anna organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 8 givenname: Galen F surname: Lynch fullname: Lynch, Galen F organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 9 givenname: Ethan G surname: McBride fullname: McBride, Ethan G organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 10 givenname: Hannah surname: Belski fullname: Belski, Hannah organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 11 givenname: Ryan surname: Gillis fullname: Gillis, Ryan organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 12 givenname: Conor surname: Grasso fullname: Grasso, Conor organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 13 givenname: Robert surname: Howard fullname: Howard, Robert organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 14 givenname: Tye surname: Johnson fullname: Johnson, Tye organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 15 givenname: Henry surname: Loeffler fullname: Loeffler, Henry organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 16 givenname: Heston surname: Smith fullname: Smith, Heston organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 17 givenname: David surname: Sullivan fullname: Sullivan, David organization: Allen Institute, Seattle, WA 98109, USA – sequence: 18 givenname: Allison surname: Williford fullname: Williford, Allison organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 19 givenname: Shiella surname: Caldejon fullname: Caldejon, Shiella organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 20 givenname: Severine surname: Durand fullname: Durand, Severine organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 21 givenname: Samuel surname: Gale fullname: Gale, Samuel organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 22 givenname: Alan surname: Guthrie fullname: Guthrie, Alan organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 23 givenname: Vivian surname: Ha fullname: Ha, Vivian organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 24 givenname: Warren surname: Han fullname: Han, Warren organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 25 givenname: Ben surname: Hardcastle fullname: Hardcastle, Ben organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 26 givenname: Chris surname: Mochizuki fullname: Mochizuki, Chris organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 27 givenname: Arjun surname: Sridhar fullname: Sridhar, Arjun organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 28 givenname: Lucas surname: Suarez fullname: Suarez, Lucas organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 29 givenname: Jackie surname: Swapp fullname: Swapp, Jackie organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 30 givenname: Joshua surname: Wilkes fullname: Wilkes, Joshua organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 31 givenname: Joshua H surname: Siegle fullname: Siegle, Joshua H organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA – sequence: 32 givenname: Colin surname: Farrell fullname: Farrell, Colin organization: Allen Institute, Seattle, WA 98109, USA – sequence: 33 givenname: Peter A surname: Groblewski fullname: Groblewski, Peter A email: peterg@alleninstitute.org organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA. Electronic address: peterg@alleninstitute.org – sequence: 34 givenname: Shawn R surname: Olsen fullname: Olsen, Shawn R email: shawno@alleninstitute.org organization: Allen Institute for Neural Dynamics, Seattle, WA 98109, USA. Electronic address: shawno@alleninstitute.org |
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| Keywords | mouse electrophysiology multi-regional recordings cranial implant craniotomy silicon probes surgical methods Neuropixels |
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| SubjectTerms | Action Potentials - physiology Animals Brain - physiology Electrodes, Implanted Electrophysiological Phenomena - physiology Electrophysiology - methods Male Mice Mice, Inbred C57BL Optogenetics - methods Printing, Three-Dimensional Skull - surgery |
| Title | SHIELD: Skull-shaped hemispheric implants enabling large-scale electrophysiology datasets in the mouse brain |
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