Suppression of 5-lipoxygenase gene is involved in triptolide-induced apoptosis in pancreatic tumor cell lines

Pancreatic adenocarcinoma is characterized by a poor prognosis and lack of response to conventional therapy. The purpose of this study was to investigate the effects of triptolide (TL) on proliferation and apoptosis of pancreatic cancer cells in vitro. We found that TL induced prominent growth inhib...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Biochimica et biophysica acta Ročník 1770; číslo 7; s. 1021 - 1027
Hlavní autoři:	Zhou, G.X., Ding, X.L., Huang, J.F., Zhang, H., Wu, S.B.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Netherlands Elsevier B.V 01.07.2007
Témata:	5-lipoxygenase Apoptosis - drug effects Arachidonate 5-Lipoxygenase - metabolism Bcl-2 Blotting, Western Caspase 3 - metabolism Caspase-3 Cell Line, Tumor Cell Proliferation - drug effects Diterpenes - pharmacology DNA Primers Enzyme-Linked Immunosorbent Assay Epoxy Compounds - pharmacology Flow Cytometry Gene Expression Regulation - drug effects Humans In Situ Nick-End Labeling Leukotriene B4 - metabolism Luciferases Pancreas - cytology Pancreas - drug effects Pancreatic tumor Phenanthrenes - pharmacology Proto-Oncogene Proteins c-bcl-2 - metabolism Reverse Transcriptase Polymerase Chain Reaction Triptolide Pancreatic tumor Bcl-2 Triptolide TL LTB4 5-lipoxygenase 5-LOX Caspase-3
ISSN:	0304-4165, 0006-3002, 1872-8006
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Abstract	Pancreatic adenocarcinoma is characterized by a poor prognosis and lack of response to conventional therapy. The purpose of this study was to investigate the effects of triptolide (TL) on proliferation and apoptosis of pancreatic cancer cells in vitro. We found that TL induced prominent growth inhibition and apoptosis in human pancreatic cell lines. In addition, TL treatment significantly down-regulated 5-lipoxygenase (5-LOX) expression, as well as downstream leukotriene B4 (LTB4) production, in these cell lines. Furthermore, overexpression of 5-LOX in SW1990 cell lines or exogenous LTB4 made them more resistant to TL-induced apoptosis, which was correlated with increased Bcl-2 expression. Taken together, these findings suggest that inhibition of the 5-LOX pathway of arachidonic acid metabolism is associated with the anti-proliferation activity of TL. We also provide evidence that TL has clinical therapeutic value for patients with pancreatic cancer.
AbstractList	Pancreatic adenocarcinoma is characterized by a poor prognosis and lack of response to conventional therapy. The purpose of this study was to investigate the effects of triptolide (TL) on proliferation and apoptosis of pancreatic cancer cells in vitro. We found that TL induced prominent growth inhibition and apoptosis in human pancreatic cell lines. In addition, TL treatment significantly down-regulated 5-lipoxygenase (5-LOX) expression, as well as downstream leukotriene B4 (LTB4) production, in these cell lines. Furthermore, overexpression of 5-LOX in SW1990 cell lines or exogenous LTB4 made them more resistant to TL-induced apoptosis, which was correlated with increased Bcl-2 expression. Taken together, these findings suggest that inhibition of the 5-LOX pathway of arachidonic acid metabolism is associated with the anti-proliferation activity of TL. We also provide evidence that TL has clinical therapeutic value for patients with pancreatic cancer. Pancreatic adenocarcinoma is characterized by a poor prognosis and lack of response to conventional therapy. The purpose of this study was to investigate the effects of triptolide (TL) on proliferation and apoptosis of pancreatic cancer cells in vitro. We found that TL induced prominent growth inhibition and apoptosis in human pancreatic cell lines. In addition, TL treatment significantly down-regulated 5-lipoxygenase (5-LOX) expression, as well as downstream leukotriene B4 (LTB4) production, in these cell lines. Furthermore, overexpression of 5-LOX in SW1990 cell lines or exogenous LTB4 made them more resistant to TL-induced apoptosis, which was correlated with increased Bcl-2 expression. Taken together, these findings suggest that inhibition of the 5-LOX pathway of arachidonic acid metabolism is associated with the anti-proliferation activity of TL. We also provide evidence that TL has clinical therapeutic value for patients with pancreatic cancer.Pancreatic adenocarcinoma is characterized by a poor prognosis and lack of response to conventional therapy. The purpose of this study was to investigate the effects of triptolide (TL) on proliferation and apoptosis of pancreatic cancer cells in vitro. We found that TL induced prominent growth inhibition and apoptosis in human pancreatic cell lines. In addition, TL treatment significantly down-regulated 5-lipoxygenase (5-LOX) expression, as well as downstream leukotriene B4 (LTB4) production, in these cell lines. Furthermore, overexpression of 5-LOX in SW1990 cell lines or exogenous LTB4 made them more resistant to TL-induced apoptosis, which was correlated with increased Bcl-2 expression. Taken together, these findings suggest that inhibition of the 5-LOX pathway of arachidonic acid metabolism is associated with the anti-proliferation activity of TL. We also provide evidence that TL has clinical therapeutic value for patients with pancreatic cancer.
Author	Huang, J.F. Wu, S.B. Zhang, H. Ding, X.L. Zhou, G.X.
Author_xml	– sequence: 1 givenname: G.X. surname: Zhou fullname: Zhou, G.X. email: zhouguoxiong@medmail.com.cn – sequence: 2 givenname: X.L. surname: Ding fullname: Ding, X.L. – sequence: 3 givenname: J.F. surname: Huang fullname: Huang, J.F. – sequence: 4 givenname: H. surname: Zhang fullname: Zhang, H. – sequence: 5 givenname: S.B. surname: Wu fullname: Wu, S.B.
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/17434678$$D View this record in MEDLINE/PubMed
BookMark	eNqFkUtr3TAQhUVJaW7S_oNStOrO7uhhye6iUEJfEMiiyVrI8rjoYkuuZF-Sf1_d3pRCF402Iw7fGYZzLshZiAEJec2gZsDUu33d9_YHhpoD6BpEDcCfkR1rNa9aAHVGdiBAVpKp5pxc5LyH8pqueUHOmZZCKt3uyPx9W5aEOfsYaBxpU01-ifcPZbHNSMtA6jP14RCnAw7lQ9fklzVOfsDKh2FzRbVLLFL-DdLFBpfQrt7RdZtjog6niU4-YH5Jno92yvjqcV6Su8-fbq--Vtc3X75dfbyunFCwVsKxRoySc9CdajrWWz42Ekc3ckQ1oG27prdOsh6AadbzThRVKOEU8A6ZuCRvT3uXFH9umFcz-3w8wwaMWzYapG6V7gr45hHc-hkHsyQ_2_Rg_gRUAHkCXIo5Jxz_ImCOPZi9OfVgjj0YEKb0UGzv_7E5v5ZIYliT9dNT5g8nM5aIDh6Tyc5jKEH7hG41Q_T_X_AL6NanAA
CitedBy_id	crossref_primary_10_1016_j_phymed_2012_02_014 crossref_primary_10_1016_j_jep_2014_06_006 crossref_primary_10_1111_j_1476_5381_2012_01969_x crossref_primary_10_1016_j_ejpb_2008_07_017 crossref_primary_10_1155_2013_172548 crossref_primary_10_3390_molecules28114316 crossref_primary_10_1016_j_biopha_2011_06_009 crossref_primary_10_1007_s12282_016_0723_2 crossref_primary_10_1016_j_heliyon_2024_e24335 crossref_primary_10_1007_s00280_011_1698_5 crossref_primary_10_1002_hsr2_2085 crossref_primary_10_1142_S0192415X13500432 crossref_primary_10_3892_or_2014_3650 crossref_primary_10_1186_1749_8546_6_27 crossref_primary_10_4103_1673_5374_191224 crossref_primary_10_1007_s00204_009_0416_0 crossref_primary_10_3389_fphar_2017_00490 crossref_primary_10_3389_fphar_2021_796300 crossref_primary_10_1371_journal_pone_0021583 crossref_primary_10_1016_j_fct_2015_10_007 crossref_primary_10_1292_jvms_11_0435 crossref_primary_10_3748_wjg_14_1504 crossref_primary_10_4049_jimmunol_1700609 crossref_primary_10_1016_j_ejmech_2019_05_032 crossref_primary_10_3727_096504013X13657689382932 crossref_primary_10_1016_j_taap_2009_05_011 crossref_primary_10_1016_j_intimp_2011_01_012 crossref_primary_10_1016_j_tips_2019_03_002 crossref_primary_10_1016_j_semcancer_2016_02_001 crossref_primary_10_1158_0008_5472_CAN_10_2858
Cites_doi	10.1016/j.chembiol.2005.09.009 10.1182/blood-2005-09-3898 10.1038/sj.onc.1204981 10.1074/jbc.274.19.13451 10.1016/j.bbrc.2004.04.201 10.1074/jbc.M009713200 10.1074/jbc.274.19.13443 10.1006/meth.2001.1262 10.1016/S0378-4274(01)00353-8 10.1016/S0304-3835(96)04554-5 10.3322/canjclin.53.1.5 10.1111/j.1365-2567.2005.02241.x 10.3109/10428190109064582 10.1038/emm.2002.64 10.3322/canjclin.46.1.5 10.1016/S0002-9440(10)64198-3 10.1006/bbrc.1999.1012 10.1016/S0006-2952(03)00282-X 10.1016/S0147-0272(96)80001-8 10.1016/S0162-3109(98)00036-8 10.1038/343282a0 10.1165/ajrcmb.26.4.4747
ContentType	Journal Article
Copyright	2007 Elsevier B.V.
Copyright_xml	– notice: 2007 Elsevier B.V.
DBID	AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8
DOI	10.1016/j.bbagen.2007.03.002
DatabaseName	CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic
DatabaseTitleList	MEDLINE - Academic MEDLINE
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Chemistry Biology
EISSN	1872-8006
EndPage	1027
ExternalDocumentID	17434678 10_1016_j_bbagen_2007_03_002 S030441650700061X
Genre	Research Support, Non-U.S. Gov't Journal Article Comparative Study
GroupedDBID	--- --K --M .~1 0R~ 1B1 1RT 1~. 1~5 23N 3O- 4.4 457 4G. 53G 5GY 5RE 5VS 7-5 71M 8P~ 9JM AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AAXUO ABEFU ABFNM ABGSF ABMAC ABUDA ABXDB ABYKQ ACDAQ ACIUM ACRLP ADBBV ADEZE ADMUD ADUVX AEBSH AEHWI AEKER AFKWA AFTJW AFXIZ AGHFR AGRDE AGUBO AGYEJ AHHHB AIEXJ AIKHN AITUG AJBFU AJOXV ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC CS3 DOVZS EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HLW HVGLF HZ~ IHE J1W KOM LX3 M41 MO0 N9A O-L O9- OAUVE OHT OZT P-8 P-9 PC. Q38 R2- ROL RPZ SBG SCC SDF SDG SDP SES SEW SPCBC SSU SSZ T5K UQL WH7 WUQ XJT XPP ~G- 9DU AAHBH AATTM AAXKI AAYWO AAYXX ABWVN ACLOT ACRPL ACVFH ADCNI ADNMO AEIPS AEUPX AFJKZ AFPUW AGQPQ AIGII AIIUN AKBMS AKRWK AKYEP ANKPU APXCP CITATION EFKBS ~HD -~X .55 .GJ AAYJJ ABJNI AFFNX AI. CGR CUY CVF ECM EIF F5P H~9 K-O MVM NPM PKN RIG TWZ UHS VH1 X7M Y6R YYP ZE2 ZGI ~KM 7X8
ID	FETCH-LOGICAL-c360t-3c153f4220796591ba2f54efcf2ee6dea895bac41b00171b2936de363c6029e13
ISICitedReferencesCount	38
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000246828700004&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	0304-4165 0006-3002
IngestDate	Thu Oct 02 07:55:20 EDT 2025 Wed Feb 19 01:43:17 EST 2025 Sat Nov 29 01:37:50 EST 2025 Tue Nov 18 21:51:58 EST 2025 Fri Feb 23 02:32:37 EST 2024
IsPeerReviewed	true
IsScholarly	true
Issue	7
Keywords	Pancreatic tumor Bcl-2 Triptolide TL LTB4 5-lipoxygenase 5-LOX Caspase-3
Language	English
License	https://www.elsevier.com/tdm/userlicense/1.0
LinkModel	OpenURL
MergedId	FETCHMERGED-LOGICAL-c360t-3c153f4220796591ba2f54efcf2ee6dea895bac41b00171b2936de363c6029e13
Notes	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
PMID	17434678
PQID	70478679
PQPubID	23479
PageCount	7
ParticipantIDs	proquest_miscellaneous_70478679 pubmed_primary_17434678 crossref_primary_10_1016_j_bbagen_2007_03_002 crossref_citationtrail_10_1016_j_bbagen_2007_03_002 elsevier_sciencedirect_doi_10_1016_j_bbagen_2007_03_002
PublicationCentury	2000
PublicationDate	2007-07-01
PublicationDateYYYYMMDD	2007-07-01
PublicationDate_xml	– month: 07 year: 2007 text: 2007-07-01 day: 01
PublicationDecade	2000
PublicationPlace	Netherlands
PublicationPlace_xml	– name: Netherlands
PublicationTitle	Biochimica et biophysica acta
PublicationTitleAlternate	Biochim Biophys Acta
PublicationYear	2007
Publisher	Elsevier B.V
Publisher_xml	– name: Elsevier B.V
References	Choi, Kim, Kim, Lee, Kwon, Suh, Park, Kwon (bib28) 2003; 66 Livak, Schmittgen (bib23) 2001; 25 Jozefowski, Biedron, Bobek, Marcinkiewicz (bib27) 2005; 116 Kiviharju, Lecane, Sellers, Peehl (bib10) 2002; 8 Lee, Chang, Qiu, Kao, Rosen (bib12) 1999; 274 Yang, Liu, Tolosa, Yang, Li (bib11) 1998; 40 Tao, Sun, Dong, Xiao, Hu, Shi, Zhu, Dai, Zhang (bib5) 1989; 102 Chen (bib6) 2001; 42 Qin, Zhu, Yang, Han, Wang (bib4) 1983; 3 Shureiqi, Lippman (bib18) 2001; 61 Qiu, Zhao, Aoki, Shi, Uyei, Nazarian, Ng, Kao (bib25) 1999; 274 Jiang, Wong, Lin, Zhu, Kung, Jiang, Yang, Lam (bib15) 2001; 20 Hennig, Ding, Tong, Schneider, Standop, Friess, Buchler, Pour, Adrian (bib20) 2002; 161 Ding, Iversen, Cluck, Knezetic, Adrian (bib21) 1999; 261 Howard (bib1) 1996; 20 Yang, Chen, Guo, Xu, Wang, Pei, Yang, Underhill, Zhang (bib9) 2003; 2 Lee, Park, Jee, Rosen (bib13) 2002; 34 Parker, Tong, Bolden, Wingo (bib2) 1996; 46 Chang, Kang, Lee, Wei, Anderson, Gotmare, Ross, Rosen (bib14) 2001; 276 Liu, Chen, Chen, Zhang, Li, Lu, Ma, Cao (bib22) 2004; 319 Wei, Adachi (bib8) 1991; 12 Jemal, Murray, Samuels, Ghafoor, Ward, Thun (bib3) 2003; 53 Chan, Cheng, Sin, Xie (bib16) 2001; 122 Carter, Mak, Schober, McQueen, Harris, Estrov, Evans, Andreeff (bib17) 2006; 108 Leuenroth, Crews (bib24) 2005; 12 Dixon, Diehl, Opas, Rands, Vickers, Evans, Gillard, Miller (bib19) 1990; 343 Silverman, Le, Baron, Hallock, Hjoberg, Shikanai, Storm van's, Auron, Lu (bib26) 2002; 26 Shamon, Pezzuto, Graves, Mehta, Wangcharoentrakul, Sangsuwan, Chaichana, Tuchinda, Cleason, Reutrakul (bib7) 1997; 112 Chang (10.1016/j.bbagen.2007.03.002_bib14) 2001; 276 Leuenroth (10.1016/j.bbagen.2007.03.002_bib24) 2005; 12 Lee (10.1016/j.bbagen.2007.03.002_bib12) 1999; 274 Jozefowski (10.1016/j.bbagen.2007.03.002_bib27) 2005; 116 Dixon (10.1016/j.bbagen.2007.03.002_bib19) 1990; 343 Qiu (10.1016/j.bbagen.2007.03.002_bib25) 1999; 274 Parker (10.1016/j.bbagen.2007.03.002_bib2) 1996; 46 Kiviharju (10.1016/j.bbagen.2007.03.002_bib10) 2002; 8 Livak (10.1016/j.bbagen.2007.03.002_bib23) 2001; 25 Chen (10.1016/j.bbagen.2007.03.002_bib6) 2001; 42 Chan (10.1016/j.bbagen.2007.03.002_bib16) 2001; 122 Choi (10.1016/j.bbagen.2007.03.002_bib28) 2003; 66 Jiang (10.1016/j.bbagen.2007.03.002_bib15) 2001; 20 Liu (10.1016/j.bbagen.2007.03.002_bib22) 2004; 319 Howard (10.1016/j.bbagen.2007.03.002_bib1) 1996; 20 Jemal (10.1016/j.bbagen.2007.03.002_bib3) 2003; 53 Wei (10.1016/j.bbagen.2007.03.002_bib8) 1991; 12 Lee (10.1016/j.bbagen.2007.03.002_bib13) 2002; 34 Hennig (10.1016/j.bbagen.2007.03.002_bib20) 2002; 161 Yang (10.1016/j.bbagen.2007.03.002_bib11) 1998; 40 Shamon (10.1016/j.bbagen.2007.03.002_bib7) 1997; 112 Qin (10.1016/j.bbagen.2007.03.002_bib4) 1983; 3 Silverman (10.1016/j.bbagen.2007.03.002_bib26) 2002; 26 Shureiqi (10.1016/j.bbagen.2007.03.002_bib18) 2001; 61 Carter (10.1016/j.bbagen.2007.03.002_bib17) 2006; 108 Tao (10.1016/j.bbagen.2007.03.002_bib5) 1989; 102 Yang (10.1016/j.bbagen.2007.03.002_bib9) 2003; 2 Ding (10.1016/j.bbagen.2007.03.002_bib21) 1999; 261
References_xml	– volume: 25 start-page: 402 year: 2001 end-page: 408 ident: bib23 article-title: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T) Method publication-title: Methods – volume: 2 start-page: 65 year: 2003 end-page: 72 ident: bib9 article-title: Triptolide inhibits the growth and metastasis of solid tumors publication-title: Mol. Cancer Ther. – volume: 34 start-page: 462 year: 2002 end-page: 468 ident: bib13 article-title: Triptolide sensitizes lung cancer cells to TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis by inhibition of NF-kappaB activation publication-title: Exp. Mol. Med. – volume: 66 start-page: 273 year: 2003 end-page: 280 ident: bib28 article-title: Immunosuppressant PG490 (triptolide) induces apoptosis through the activation of caspase-3 and down-regulation of XIAP in U937 cells publication-title: Biochem. Pharmacol. – volume: 20 start-page: 281 year: 1996 end-page: 328 ident: bib1 article-title: Pancreatic adenocarcinoma publication-title: Curr. Probl. Cancer – volume: 3 start-page: 131 year: 1983 end-page: 132 ident: bib4 article-title: Clinical observations on publication-title: J. Tradit. Chin. Med. – volume: 274 start-page: 13443 year: 1999 end-page: 13450 ident: bib25 article-title: Immunosuppressant PG490 (triptolide) inhibits T-cell interleukin-2 expression at the level of purine-box/nuclear factor of activated T-cells and NF-kappaB transcriptional activation publication-title: J. Biol. Chem. – volume: 276 start-page: 2221 year: 2001 end-page: 2227 ident: bib14 article-title: Triptolide and chemotherapy cooperate in tumor cell apoptosis. A role for the p53 pathway publication-title: J. Biol. Chem. – volume: 343 start-page: 282 year: 1990 end-page: 284 ident: bib19 article-title: Requirement of a 5-lipoxygenase-activating protein for leukotriene synthesis publication-title: Nature – volume: 26 start-page: 475 year: 2002 end-page: 483 ident: bib26 article-title: Cloning and functional analysis of the mouse 5-lipoxygenase promoter publication-title: Am. J. Respir. Cell Mol. Biol. – volume: 42 start-page: 253 year: 2001 end-page: 265 ident: bib6 article-title: Triptolide, a novel immunosuppressive and anti-inflammatory agent purified from a Chinese herb publication-title: Leuk. Lymphoma – volume: 319 start-page: 980 year: 2004 end-page: 986 ident: bib22 article-title: Triptolide (PG-490) induces apoptosis of dendritic cells through sequential p38 MAP kinase phosphorylation and caspase 3 activation publication-title: Biochem. Biophys. Res. Commun. – volume: 102 start-page: 327 year: 1989 end-page: 332 ident: bib5 article-title: A prospective, controlled, double-blind, cross-over study of publication-title: Chin. Med. J. (Engl.) – volume: 112 start-page: 113 year: 1997 end-page: 117 ident: bib7 article-title: Evaluation of the mutagenic, cytotoxic, and antitumor potential of triptolide, a highly oxygenated diterpene isolated from publication-title: Cancer Lett. – volume: 108 start-page: 630 year: 2006 end-page: 637 ident: bib17 article-title: Triptolide induces caspase-dependent cell death mediated via the mitochondrial pathway in leukemic cells publication-title: Blood – volume: 12 start-page: 406 year: 1991 end-page: 410 ident: bib8 article-title: Inhibitory effect of triptolide on colony formation of breast and stomach cancer cell lines publication-title: Zhongguo Yao Li Xue. Bao. – volume: 274 start-page: 13451 year: 1999 end-page: 13455 ident: bib12 article-title: PG490 (triptolide) cooperates with tumor necrosis factor-alpha to induce apoptosis in tumor cells publication-title: J. Biol. Chem. – volume: 161 start-page: 421 year: 2002 end-page: 428 ident: bib20 article-title: 5-Lipoxygenase and leukotriene B(4) receptor are expressed in human pancreatic cancers but not in pancreatic ducts in normal tissue publication-title: Am. J. Pathol. – volume: 261 start-page: 218 year: 1999 end-page: 223 ident: bib21 article-title: Lipoxygenase inhibitors abolish proliferation of human pancreatic cancer cells publication-title: Biochem. Biophys. Res. Commun. – volume: 40 start-page: 139 year: 1998 end-page: 149 ident: bib11 article-title: Triptolide induces apoptotic death of T lymphocyte publication-title: Immunopharmacology – volume: 53 start-page: 5 year: 2003 end-page: 26 ident: bib3 article-title: Cancer statistics, 2003 CA Cancer publication-title: J. Clin. – volume: 20 start-page: 8009 year: 2001 end-page: 8018 ident: bib15 article-title: Functional p53 is required for triptolide-induced apoptosis and AP-1 and nuclear factor-kappaB activation in gastric cancer cells publication-title: Oncogene – volume: 122 start-page: 81 year: 2001 end-page: 87 ident: bib16 article-title: Triptolide induced cytotoxic effects on human promyelocytic leukemia, T cell lymphoma and human hepatocellular carcinoma cell lines publication-title: Toxicol. Lett. – volume: 116 start-page: 418 year: 2005 end-page: 428 ident: bib27 article-title: Leukotrienes modulate cytokine release from dendritic cells publication-title: Immunology – volume: 8 start-page: 2666 year: 2002 end-page: 2674 ident: bib10 article-title: Antiproliferative and proapoptotic activities of triptolide (PG490), a natural product entering clinical trials, on primary cultures of human prostatic epithelial cells publication-title: Clin. Cancer Res. – volume: 12 start-page: 1259 year: 2005 end-page: 1268 ident: bib24 article-title: Studies on calcium dependence reveal multiple modes of action for triptolide publication-title: Chem. Biol. – volume: 46 start-page: 5 year: 1996 end-page: 27 ident: bib2 article-title: Cancer statistics, 1996 CA Cancer publication-title: J. Clin. – volume: 61 start-page: 6307 year: 2001 end-page: 6312 ident: bib18 article-title: Lipoxygenase modulation to reverse carcinogenesis publication-title: Cancer Res. – volume: 12 start-page: 406 year: 1991 ident: 10.1016/j.bbagen.2007.03.002_bib8 article-title: Inhibitory effect of triptolide on colony formation of breast and stomach cancer cell lines publication-title: Zhongguo Yao Li Xue. Bao. – volume: 8 start-page: 2666 year: 2002 ident: 10.1016/j.bbagen.2007.03.002_bib10 article-title: Antiproliferative and proapoptotic activities of triptolide (PG490), a natural product entering clinical trials, on primary cultures of human prostatic epithelial cells publication-title: Clin. Cancer Res. – volume: 102 start-page: 327 year: 1989 ident: 10.1016/j.bbagen.2007.03.002_bib5 article-title: A prospective, controlled, double-blind, cross-over study of Tripterygium wilfordii hook F in treatment of rheumatoid arthritis publication-title: Chin. Med. J. (Engl.) – volume: 3 start-page: 131 year: 1983 ident: 10.1016/j.bbagen.2007.03.002_bib4 article-title: Clinical observations on Tripterygium wilfordii in treatment of 26 cases of discoid lupus erythematosus publication-title: J. Tradit. Chin. Med. – volume: 12 start-page: 1259 year: 2005 ident: 10.1016/j.bbagen.2007.03.002_bib24 article-title: Studies on calcium dependence reveal multiple modes of action for triptolide publication-title: Chem. Biol. doi: 10.1016/j.chembiol.2005.09.009 – volume: 108 start-page: 630 year: 2006 ident: 10.1016/j.bbagen.2007.03.002_bib17 article-title: Triptolide induces caspase-dependent cell death mediated via the mitochondrial pathway in leukemic cells publication-title: Blood doi: 10.1182/blood-2005-09-3898 – volume: 20 start-page: 8009 year: 2001 ident: 10.1016/j.bbagen.2007.03.002_bib15 article-title: Functional p53 is required for triptolide-induced apoptosis and AP-1 and nuclear factor-kappaB activation in gastric cancer cells publication-title: Oncogene doi: 10.1038/sj.onc.1204981 – volume: 274 start-page: 13451 year: 1999 ident: 10.1016/j.bbagen.2007.03.002_bib12 article-title: PG490 (triptolide) cooperates with tumor necrosis factor-alpha to induce apoptosis in tumor cells publication-title: J. Biol. Chem. doi: 10.1074/jbc.274.19.13451 – volume: 319 start-page: 980 year: 2004 ident: 10.1016/j.bbagen.2007.03.002_bib22 article-title: Triptolide (PG-490) induces apoptosis of dendritic cells through sequential p38 MAP kinase phosphorylation and caspase 3 activation publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/j.bbrc.2004.04.201 – volume: 276 start-page: 2221 year: 2001 ident: 10.1016/j.bbagen.2007.03.002_bib14 article-title: Triptolide and chemotherapy cooperate in tumor cell apoptosis. A role for the p53 pathway publication-title: J. Biol. Chem. doi: 10.1074/jbc.M009713200 – volume: 274 start-page: 13443 year: 1999 ident: 10.1016/j.bbagen.2007.03.002_bib25 article-title: Immunosuppressant PG490 (triptolide) inhibits T-cell interleukin-2 expression at the level of purine-box/nuclear factor of activated T-cells and NF-kappaB transcriptional activation publication-title: J. Biol. Chem. doi: 10.1074/jbc.274.19.13443 – volume: 25 start-page: 402 year: 2001 ident: 10.1016/j.bbagen.2007.03.002_bib23 article-title: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T) Method publication-title: Methods doi: 10.1006/meth.2001.1262 – volume: 122 start-page: 81 year: 2001 ident: 10.1016/j.bbagen.2007.03.002_bib16 article-title: Triptolide induced cytotoxic effects on human promyelocytic leukemia, T cell lymphoma and human hepatocellular carcinoma cell lines publication-title: Toxicol. Lett. doi: 10.1016/S0378-4274(01)00353-8 – volume: 112 start-page: 113 year: 1997 ident: 10.1016/j.bbagen.2007.03.002_bib7 article-title: Evaluation of the mutagenic, cytotoxic, and antitumor potential of triptolide, a highly oxygenated diterpene isolated from Tripterygium wilfordii publication-title: Cancer Lett. doi: 10.1016/S0304-3835(96)04554-5 – volume: 53 start-page: 5 year: 2003 ident: 10.1016/j.bbagen.2007.03.002_bib3 article-title: Cancer statistics, 2003 CA Cancer publication-title: J. Clin. doi: 10.3322/canjclin.53.1.5 – volume: 2 start-page: 65 year: 2003 ident: 10.1016/j.bbagen.2007.03.002_bib9 article-title: Triptolide inhibits the growth and metastasis of solid tumors publication-title: Mol. Cancer Ther. – volume: 116 start-page: 418 year: 2005 ident: 10.1016/j.bbagen.2007.03.002_bib27 article-title: Leukotrienes modulate cytokine release from dendritic cells publication-title: Immunology doi: 10.1111/j.1365-2567.2005.02241.x – volume: 42 start-page: 253 year: 2001 ident: 10.1016/j.bbagen.2007.03.002_bib6 article-title: Triptolide, a novel immunosuppressive and anti-inflammatory agent purified from a Chinese herb Tripterygium wilfordii Hook F publication-title: Leuk. Lymphoma doi: 10.3109/10428190109064582 – volume: 34 start-page: 462 year: 2002 ident: 10.1016/j.bbagen.2007.03.002_bib13 article-title: Triptolide sensitizes lung cancer cells to TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis by inhibition of NF-kappaB activation publication-title: Exp. Mol. Med. doi: 10.1038/emm.2002.64 – volume: 46 start-page: 5 year: 1996 ident: 10.1016/j.bbagen.2007.03.002_bib2 article-title: Cancer statistics, 1996 CA Cancer publication-title: J. Clin. doi: 10.3322/canjclin.46.1.5 – volume: 161 start-page: 421 year: 2002 ident: 10.1016/j.bbagen.2007.03.002_bib20 article-title: 5-Lipoxygenase and leukotriene B(4) receptor are expressed in human pancreatic cancers but not in pancreatic ducts in normal tissue publication-title: Am. J. Pathol. doi: 10.1016/S0002-9440(10)64198-3 – volume: 261 start-page: 218 year: 1999 ident: 10.1016/j.bbagen.2007.03.002_bib21 article-title: Lipoxygenase inhibitors abolish proliferation of human pancreatic cancer cells publication-title: Biochem. Biophys. Res. Commun. doi: 10.1006/bbrc.1999.1012 – volume: 66 start-page: 273 year: 2003 ident: 10.1016/j.bbagen.2007.03.002_bib28 article-title: Immunosuppressant PG490 (triptolide) induces apoptosis through the activation of caspase-3 and down-regulation of XIAP in U937 cells publication-title: Biochem. Pharmacol. doi: 10.1016/S0006-2952(03)00282-X – volume: 20 start-page: 281 year: 1996 ident: 10.1016/j.bbagen.2007.03.002_bib1 article-title: Pancreatic adenocarcinoma publication-title: Curr. Probl. Cancer doi: 10.1016/S0147-0272(96)80001-8 – volume: 40 start-page: 139 year: 1998 ident: 10.1016/j.bbagen.2007.03.002_bib11 article-title: Triptolide induces apoptotic death of T lymphocyte publication-title: Immunopharmacology doi: 10.1016/S0162-3109(98)00036-8 – volume: 61 start-page: 6307 year: 2001 ident: 10.1016/j.bbagen.2007.03.002_bib18 article-title: Lipoxygenase modulation to reverse carcinogenesis publication-title: Cancer Res. – volume: 343 start-page: 282 year: 1990 ident: 10.1016/j.bbagen.2007.03.002_bib19 article-title: Requirement of a 5-lipoxygenase-activating protein for leukotriene synthesis publication-title: Nature doi: 10.1038/343282a0 – volume: 26 start-page: 475 year: 2002 ident: 10.1016/j.bbagen.2007.03.002_bib26 article-title: Cloning and functional analysis of the mouse 5-lipoxygenase promoter publication-title: Am. J. Respir. Cell Mol. Biol. doi: 10.1165/ajrcmb.26.4.4747
SSID	ssj0000595 ssj0025309
Score	2.0499892
Snippet	Pancreatic adenocarcinoma is characterized by a poor prognosis and lack of response to conventional therapy. The purpose of this study was to investigate the...
SourceID	proquest pubmed crossref elsevier
SourceType	Aggregation Database Index Database Enrichment Source Publisher
StartPage	1021
SubjectTerms	5-lipoxygenase Apoptosis - drug effects Arachidonate 5-Lipoxygenase - metabolism Bcl-2 Blotting, Western Caspase 3 - metabolism Caspase-3 Cell Line, Tumor Cell Proliferation - drug effects Diterpenes - pharmacology DNA Primers Enzyme-Linked Immunosorbent Assay Epoxy Compounds - pharmacology Flow Cytometry Gene Expression Regulation - drug effects Humans In Situ Nick-End Labeling Leukotriene B4 - metabolism Luciferases Pancreas - cytology Pancreas - drug effects Pancreatic tumor Phenanthrenes - pharmacology Proto-Oncogene Proteins c-bcl-2 - metabolism Reverse Transcriptase Polymerase Chain Reaction Triptolide
Title	Suppression of 5-lipoxygenase gene is involved in triptolide-induced apoptosis in pancreatic tumor cell lines
URI	https://dx.doi.org/10.1016/j.bbagen.2007.03.002 https://www.ncbi.nlm.nih.gov/pubmed/17434678 https://www.proquest.com/docview/70478679
Volume	1770
WOSCitedRecordID	wos000246828700004&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVESC databaseName: Elsevier SD Freedom Collection Journals 2021 customDbUrl: eissn: 1872-8006 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000595 issn: 0304-4165 databaseCode: AIEXJ dateStart: 19950118 isFulltext: true titleUrlDefault: https://www.sciencedirect.com providerName: Elsevier
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwELbKFgQXBIXC8ig-cIsS5W3nWFWtSgWrHooUcYnsrK2m2iarzWa1_Vv8QsZ2HruUUnrgEiXeJPJmvng-T8bzIfQ5YnEydafCzimjdugxYdOQJXYexB6XVESCci02QSYTmqbJ-c7Oz24tzGpGypKu18n8v5oa2sDYaunsA8zd3xQaYB-MDlswO2z_yfBKp9Mkt2omGNmzYl6tb-B8cFhKMVkoEfOihHFpJaY6z1ENHNWsgGcJM_RGZQSweQVNdWGyzAEZmlvm1rK5rhaWivZbip_WW9-Eiyq_LFT5AUssLV5UJmrCVL0O5nQFrq264Sr203P5H5dVo6PzTur0tLpVWkmdr84AvDa0feacOMPFbeOpsxW_IH2ua79uyw0BJEYxoh-TiZETadFHNoZYpUW-4a7hkPzRFZioxJXDOZxWtsUqVTlbf3B93ef-3zxin6fYpcBdZeYuSrSTZG6Q6fqluz6JEjpCu4dfjtOzwf9HWuun_1_dgk2dVXi7N3cRorsmPJr4XLxAz9sZCz40SHuJdkS5h54YDdObPfT0qJMMfIWuN7CHK4m3sYcV9nBR4w57sINvYw_32FO_D9jDGntYYQ9r7L1G30-OL45O7VbPQ7357tIOcnCvMvR9l6gylh5nvoxCIXPpCxFPBaNJxFkeejoy4XFgotAaxEEeu34ivGAfjcqqFG8RTmDWEEzdUJKIhcSnnHoyoS6nAZMylPkYBd1DzfK22L3SXJllfzPpGNn9VXNT7OWe80lnr6wlrIaIZgDCe6781Jk3AxupR8dKUTV1RlS5rJgkY_TGWH3oCZB9oDX03QN7-R49G967D2i0XDTiI3qcr5ZFvThAj0hKD1oMw9Hk_Nsv5K_P5A
linkProvider	Elsevier
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Suppression+of+5-lipoxygenase+gene+is+involved+in+triptolide-induced+apoptosis+in+pancreatic+tumor+cell+lines&rft.jtitle=Biochimica+et+biophysica+acta.+General+subjects&rft.au=Zhou%2C+G.X.&rft.au=Ding%2C+X.L.&rft.au=Huang%2C+J.F.&rft.au=Zhang%2C+H.&rft.date=2007-07-01&rft.issn=0304-4165&rft.volume=1770&rft.issue=7&rft.spage=1021&rft.epage=1027&rft_id=info:doi/10.1016%2Fj.bbagen.2007.03.002&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_j_bbagen_2007_03_002
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0304-4165&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0304-4165&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0304-4165&client=summon