Doxycycline‐loaded carboxymethyl cellulose/sodium alginate/gelatin hydrogel: An approach for enhancing pressure ulcer healing in a rat model

Pressure ulcers, or bedsores, are created by areas of the skin under prolonged pressure and can lead to skin and underlying tissue damage. The present study evaluated the effects of carboxymethyl cellulose/sodium alginate/gelatin (CMC/Alg/Gel) hydrogel containing doxycycline (DOX) on improving the h...

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Published in:Journal of biomedical materials research. Part A Vol. 112; no. 12; pp. 2289 - 2300
Main Authors: Aldaghi, Niloofar, Kamalabadi‐Farahani, Mohammad, Alizadeh, Morteza, Salehi, Majid
Format: Journal Article
Language:English
Published: Hoboken, USA John Wiley & Sons, Inc 01.12.2024
Wiley Subscription Services, Inc
Subjects:
Alginates - chemistry
Alginates - pharmacology
Alginic acid
Animals
Antibiotics
bedsore
Biocompatibility
Carboxymethyl cellulose
Carboxymethylcellulose
Carboxymethylcellulose Sodium - chemistry
Cell viability
Cellulose
Damage assessment
Disease Models, Animal
Doxycycline
Doxycycline - pharmacology
Gelatin
Gelatin - chemistry
Gelatinase A
Gelatinase B
Gene expression
Growth factors
Healing
hydrogel
Hydrogels
Hydrogels - chemistry
Hydrogels - pharmacology
Male
mRNA
Pressure
Pressure effects
Pressure Ulcer - drug therapy
Pressure Ulcer - pathology
Pressure ulcers
Rats
Rats, Sprague-Dawley
Skin
Sodium
Sodium alginate
Ulcers
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A - metabolism
Weight loss
Wound healing
Wound Healing - drug effects
pressure ulcers
wound healing
bedsore
doxycycline
hydrogel
ISSN:1549-3296, 1552-4965, 1552-4965
Online Access:Get full text
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Summary:Pressure ulcers, or bedsores, are created by areas of the skin under prolonged pressure and can lead to skin and underlying tissue damage. The present study evaluated the effects of carboxymethyl cellulose/sodium alginate/gelatin (CMC/Alg/Gel) hydrogel containing doxycycline (DOX) on improving the healing process of pressure ulcers. The magnet was used to apply pressure on the dorsum skin rat to induce a pressure ulcer model. Then sterile gauze, CMC/Alg/Gel, and CMC/Alg/Gel/1% w/v DOX hydrogels were used to cover the wounds. Blood compatibility, weight loss, cytocompatibility, drug release rate, cell viability, wound closure, and re‐epithelialization were evaluated in all animals on the 14th day after treatment. In vivo results and histopathological evaluation showed 56.66% wound closure and the highest re‐epithelialization in the CMC/Alg/Gel/1% w/v DOX hydrogel group (14 days after treatment). Furthermore, real‐time PCR results indicated that the hydrogel containing DOX significantly decreased the expression of the MMP family consisting of MMP2 and MMP9 mRNA and also increased the expression of vascular endothelial growth factor VEGF mRNA. This study suggested that the addition of DOX, an antibiotic and MMP inhibitor, to hydrogels may be effective in the healing process of pressure ulcers.
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ISSN:1549-3296
1552-4965
1552-4965
DOI:10.1002/jbm.a.37778