Identification and risk assessment of human and veterinary antibiotics in the wastewater treatment plants and the adjacent sea in Tunisia

In the following study, we came up with and validated a prompt, sensitive and precise method for the simultaneous determination of 56 antimicrobial drugs (tetracyclines, sulfonamides, β-lactams, macrolides and quinolones) using the ultra-performance liquid chromatography-tandem mass spectrometry (UP...

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Bibliographic Details
Published in:Water science and technology Vol. 76; no. 11; pp. 3000 - 3021
Main Authors: Tahrani, Leyla, Van Loco, Joris, Anthonissen, Roel, Verschaeve, Luc, Ben Mansour, Hedi, Reyns, Tim
Format: Journal Article
Language:English
Published: England IWA Publishing 01.12.2017
Subjects:
Animals
Anti-Bacterial Agents - chemistry
Antibiotics
Assaying
Batteries
Chromatography
Chromatography, Liquid - methods
Drugs
Ecosystems
Genotoxicity
Hepatocellular carcinoma
Hepatocytes
Human performance
Humans
Hypoxis
Liquid chromatography
Liver
Liver cancer
Mass spectrometry
Mass spectroscopy
Mediterranean Sea
Metabolites
Methods
Neoplasms
Organic compounds
Pharmaceutical industry
Power plants
Quinolones
Risk Assessment
Salmonella
Salmonella Typhimurium
Scientific imaging
Sulfonamides
Tandem Mass Spectrometry - methods
Tetracyclines
Toxicity
Tunisia
Veterinary Drugs
Veterinary medicine
Waste Disposal Facilities
Waste Disposal, Fluid
Waste Water - analysis
Wastewater
Wastewater treatment
Wastewater treatment plants
Water Pollutants, Chemical - chemistry
Water treatment
ISSN:0273-1223, 1996-9732
Online Access:Get full text
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Summary:In the following study, we came up with and validated a prompt, sensitive and precise method for the simultaneous determination of 56 antimicrobial drugs (tetracyclines, sulfonamides, β-lactams, macrolides and quinolones) using the ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). This method was implemented with success to determine antibiotics in samples collected from four wastewater treatment plants and five coasts in Tunisia. Results showed the presence of high concentrations of antibiotics that ranged from 0.1 to 646 ng mL−1, which can induce many negative impacts on health and the environment. For this reason, we have opted to evaluate toxicity of wastewater samples using a battery of biotests. In fact, genotoxicity was assessed using three tests: Vitotox, comet and micronucleus assays. The input and output of wastewater treatment plants induced a strongly genotoxic effect on the Salmonella typhimurium TA104 prokaryotic Vitotox assay. This result was confirmed using the comet and the micronucleus assays performed on the human liver hepatocellular carcinoma cells. The genotoxic power of the tested wastewater treatment plants' samples could be attributed to the presence of the higher quantities of antibiotics that are detected in these samples and to the antibiotic and organic compound cocktails.
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ISSN:0273-1223
1996-9732
DOI:10.2166/wst.2017.465