Detection of human 11β-hydroxysteroid dehydrogenase isoforms using reverse-transcriptase-polymerase chain reaction and localization of the type 2 isoform to renal collecting ducts

11β-Hydroxysteroid dehydrogenase (11β-HSD), responsible for the interconversion of hormonally active cortisol to inactive cortisone, dictates specificity for the mineralocorticoid receptor (MR) in the distal nephron and colon. Two isoforms of human 11β-HSD have been cloned, an NADP(H)-dependent (typ...

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Bibliographic Details
Published in:	Molecular and cellular endocrinology Vol. 110; no. 1; pp. R7 - R12
Main Authors:	Whorwood, C.B., Mason, J.I., Ricketts, M.L., Howie, A.J., Stewart, P.M.
Format:	Journal Article
Language:	English
Published:	Ireland Elsevier Ireland Ltd 28.04.1995
Subjects:	11-beta-Hydroxysteroid Dehydrogenases 11β-Hydroxysteroid dehydrogenase Cortisol Glucocorticoid Humans Hydroxysteroid Dehydrogenases - analysis Hydroxysteroid Dehydrogenases - genetics In Situ Hybridization Isoenzymes - analysis Isoenzymes - genetics Kidney Cortex - enzymology Kidney Medulla - enzymology Kidney Tubules, Collecting - enzymology Metabolism Mineralocorticoid NAD - pharmacology Organ Specificity Polymerase Chain Reaction Receptors RNA Probes RNA, Antisense RNA, Messenger - analysis RNA-Directed DNA Polymerase Tissue Distribution Receptors Cortisol Metabolism Mineralocorticoid Glucocorticoid 11β-Hydroxysteroid dehydrogenase
ISSN:	0303-7207, 1872-8057
Online Access:	Get full text
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