Proinflammatory cytokine profiling of tears from dry eye patients by means of antibody microarrays

In the pathogenesis of keratoconjunctivitis sicca, immune processes are thought to play an important role. However, the exact details of the pathomechanisms are still unknown. In this study, the expression patterns of proinflammatory cytokines in the tears of patients with different subtypes of dry...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Investigative ophthalmology & visual science Jg. 52; H. 10; S. 7725
Hauptverfasser: Boehm, Nils, Riechardt, Aline I, Wiegand, Michaela, Pfeiffer, Norbert, Grus, Franz H
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 29.09.2011
Schlagworte:
Adolescent
Adult
Aged
Child
Child, Preschool
Dry Eye Syndromes - classification
Dry Eye Syndromes - metabolism
Eye Proteins - metabolism
Female
Humans
Infant
Interferon-gamma - metabolism
Interleukin-1beta - metabolism
Interleukin-6 - metabolism
Male
Middle Aged
Protein Array Analysis
Tears - chemistry
Tears - metabolism
Tumor Necrosis Factor-alpha - metabolism
Young Adult
ISSN:1552-5783, 1552-5783
Online-Zugang:Weitere Angaben
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Abstract In the pathogenesis of keratoconjunctivitis sicca, immune processes are thought to play an important role. However, the exact details of the pathomechanisms are still unknown. In this study, the expression patterns of proinflammatory cytokines in the tears of patients with different subtypes of dry eye were analyzed. One hundred forty-three subjects subdivided into healthy controls (CTRL, n = 38), patients with aqueous-deficient dry eye (DRYaq, n = 35), patients with changes of the lipid layer (DRYlip, n = 36), and patients with a combination of both (DRYaplip, n = 34) were examined. Expression patterns of proteins (e.g., IL-1β, IL-6, ITNF-α, and IFN-γ) were examined using an advanced antibody microarray approach. Several highly significant differences in the cytokine levels of dry eye patients compared with healthy controls were detected. Patients with DRYaq or those with DRYaplip showed elevated levels for most of the tested proteins. For example, IL-1β was found to be elevated 2.4-fold in DRYaq patients and 2.75-fold in DRYaqlip patients (both P < 8.00E-6). The detected amounts of protein in DRYlip patients and in healthy controls showed only minimal differences (fold increase/decrease for all proteins >1.2; P > 5.00E-1). The similarity between the profiles of healthy controls and DRYlip patients justifies the assumption that the pathomechanism of this dry eye subtype is based on mechanisms other than inflammation, whereas it seems to be the case for DRYaq patients.
AbstractList In the pathogenesis of keratoconjunctivitis sicca, immune processes are thought to play an important role. However, the exact details of the pathomechanisms are still unknown. In this study, the expression patterns of proinflammatory cytokines in the tears of patients with different subtypes of dry eye were analyzed.PURPOSEIn the pathogenesis of keratoconjunctivitis sicca, immune processes are thought to play an important role. However, the exact details of the pathomechanisms are still unknown. In this study, the expression patterns of proinflammatory cytokines in the tears of patients with different subtypes of dry eye were analyzed.One hundred forty-three subjects subdivided into healthy controls (CTRL, n = 38), patients with aqueous-deficient dry eye (DRYaq, n = 35), patients with changes of the lipid layer (DRYlip, n = 36), and patients with a combination of both (DRYaplip, n = 34) were examined. Expression patterns of proteins (e.g., IL-1β, IL-6, ITNF-α, and IFN-γ) were examined using an advanced antibody microarray approach.METHODSOne hundred forty-three subjects subdivided into healthy controls (CTRL, n = 38), patients with aqueous-deficient dry eye (DRYaq, n = 35), patients with changes of the lipid layer (DRYlip, n = 36), and patients with a combination of both (DRYaplip, n = 34) were examined. Expression patterns of proteins (e.g., IL-1β, IL-6, ITNF-α, and IFN-γ) were examined using an advanced antibody microarray approach.Several highly significant differences in the cytokine levels of dry eye patients compared with healthy controls were detected. Patients with DRYaq or those with DRYaplip showed elevated levels for most of the tested proteins. For example, IL-1β was found to be elevated 2.4-fold in DRYaq patients and 2.75-fold in DRYaqlip patients (both P < 8.00E-6). The detected amounts of protein in DRYlip patients and in healthy controls showed only minimal differences (fold increase/decrease for all proteins >1.2; P > 5.00E-1).RESULTSSeveral highly significant differences in the cytokine levels of dry eye patients compared with healthy controls were detected. Patients with DRYaq or those with DRYaplip showed elevated levels for most of the tested proteins. For example, IL-1β was found to be elevated 2.4-fold in DRYaq patients and 2.75-fold in DRYaqlip patients (both P < 8.00E-6). The detected amounts of protein in DRYlip patients and in healthy controls showed only minimal differences (fold increase/decrease for all proteins >1.2; P > 5.00E-1).The similarity between the profiles of healthy controls and DRYlip patients justifies the assumption that the pathomechanism of this dry eye subtype is based on mechanisms other than inflammation, whereas it seems to be the case for DRYaq patients.CONCLUSIONSThe similarity between the profiles of healthy controls and DRYlip patients justifies the assumption that the pathomechanism of this dry eye subtype is based on mechanisms other than inflammation, whereas it seems to be the case for DRYaq patients.
In the pathogenesis of keratoconjunctivitis sicca, immune processes are thought to play an important role. However, the exact details of the pathomechanisms are still unknown. In this study, the expression patterns of proinflammatory cytokines in the tears of patients with different subtypes of dry eye were analyzed. One hundred forty-three subjects subdivided into healthy controls (CTRL, n = 38), patients with aqueous-deficient dry eye (DRYaq, n = 35), patients with changes of the lipid layer (DRYlip, n = 36), and patients with a combination of both (DRYaplip, n = 34) were examined. Expression patterns of proteins (e.g., IL-1β, IL-6, ITNF-α, and IFN-γ) were examined using an advanced antibody microarray approach. Several highly significant differences in the cytokine levels of dry eye patients compared with healthy controls were detected. Patients with DRYaq or those with DRYaplip showed elevated levels for most of the tested proteins. For example, IL-1β was found to be elevated 2.4-fold in DRYaq patients and 2.75-fold in DRYaqlip patients (both P < 8.00E-6). The detected amounts of protein in DRYlip patients and in healthy controls showed only minimal differences (fold increase/decrease for all proteins >1.2; P > 5.00E-1). The similarity between the profiles of healthy controls and DRYlip patients justifies the assumption that the pathomechanism of this dry eye subtype is based on mechanisms other than inflammation, whereas it seems to be the case for DRYaq patients.
Author Wiegand, Michaela
Grus, Franz H
Boehm, Nils
Riechardt, Aline I
Pfeiffer, Norbert
Author_xml – sequence: 1
  givenname: Nils
  surname: Boehm
  fullname: Boehm, Nils
  organization: Experimental Ophthalmology, Department of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
– sequence: 2
  givenname: Aline I
  surname: Riechardt
  fullname: Riechardt, Aline I
– sequence: 3
  givenname: Michaela
  surname: Wiegand
  fullname: Wiegand, Michaela
– sequence: 4
  givenname: Norbert
  surname: Pfeiffer
  fullname: Pfeiffer, Norbert
– sequence: 5
  givenname: Franz H
  surname: Grus
  fullname: Grus, Franz H
BackLink https://www.ncbi.nlm.nih.gov/pubmed/21775656$$D View this record in MEDLINE/PubMed
BookMark eNpNkM1LxDAUxIOsuB968yy5eao2aZO0R1n8ggU96Lm8tC8SbZM1SYX-91ZcwdMb3vwYhlmThfMOCTln-RVjUl1b_xVnlSku5RFZMSF4JlRVLP7pJVnH-J7nnDGen5AlZ0oJKeSK6OfgrTM9DAMkHybaTsl_WId0H7yxvXVv1BuaEEKkJviBdjOE0-xDsuhSpHqiA4KLPxy4ZLXv5o9tg4cQYIqn5NhAH_HscDfk9e72ZfuQ7Z7uH7c3u6wthEyZ7oQoBC_KArHTsm6lQqharGtlQOqiBIlgSuBlCVUtawbSVCVAx3RnOFR8Qy5_c-fmnyPG1Aw2ttj34NCPsalqUQk1TzKTFwdy1AN2zT7YAcLU_M3CvwE2Qmjj
CitedBy_id crossref_primary_10_1097_ICO_0000000000000256
crossref_primary_10_1136_bjophthalmol_2012_302868
crossref_primary_10_1016_j_exer_2022_109121
crossref_primary_10_1371_journal_pone_0137186
crossref_primary_10_1002_prca_201400084
crossref_primary_10_1097_ICL_0b013e3182a2f8fa
crossref_primary_10_1016_j_exer_2022_109127
crossref_primary_10_1016_j_jtos_2018_05_001
crossref_primary_10_1167_iovs_65_2_16
crossref_primary_10_1177_1120672118795399
crossref_primary_10_1089_jop_2015_0029
crossref_primary_10_1186_s40662_014_0006_y
crossref_primary_10_1016_j_exer_2013_08_013
crossref_primary_10_1080_08164622_2025_2485236
crossref_primary_10_1016_j_jtos_2018_04_007
crossref_primary_10_1016_j_bbmt_2015_08_020
crossref_primary_10_3390_bios14100483
crossref_primary_10_1097_ICL_0000000000000158
crossref_primary_10_1016_j_exer_2013_07_015
crossref_primary_10_1080_02713683_2022_2162088
crossref_primary_10_1002_mas_21612
crossref_primary_10_1097_ICO_0000000000001238
crossref_primary_10_1155_2014_320906
crossref_primary_10_1016_j_jtos_2013_04_004
crossref_primary_10_1186_s13167_016_0065_3
crossref_primary_10_1080_02713683_2019_1604972
crossref_primary_10_4103_ijo_IJO_405_17
crossref_primary_10_1016_j_exer_2022_109035
crossref_primary_10_1016_j_jtos_2017_05_011
crossref_primary_10_1111_jcmm_12495
crossref_primary_10_3390_pharmaceutics15030990
crossref_primary_10_1155_2014_570685
crossref_primary_10_1007_s13167_021_00265_y
crossref_primary_10_1016_j_ajo_2014_03_002
crossref_primary_10_1016_j_ajo_2019_01_002
crossref_primary_10_1097_ICL_0000000000000182
crossref_primary_10_1016_j_clae_2017_05_008
crossref_primary_10_1016_j_oftal_2016_12_009
crossref_primary_10_1016_j_jtos_2014_02_002
crossref_primary_10_1016_j_oftale_2016_12_005
crossref_primary_10_4252_wjsc_v17_i4_101891
crossref_primary_10_1002_jcp_27895
crossref_primary_10_3390_bioengineering11010039
crossref_primary_10_3390_ijms25074019
crossref_primary_10_1007_s12020_017_1467_2
crossref_primary_10_1186_s12929_022_00821_2
crossref_primary_10_1016_j_jtos_2014_01_004
crossref_primary_10_1016_j_jtos_2021_07_006
crossref_primary_10_11603_mcch_2410_681X_2025_i2_15521
crossref_primary_10_1167_iovs_66_11_76
crossref_primary_10_1016_j_baga_2015_05_001
crossref_primary_10_1016_j_cyto_2018_05_013
crossref_primary_10_1371_journal_pone_0143131
crossref_primary_10_3390_ijms20010009
crossref_primary_10_1097_ACI_0b013e328357b488
crossref_primary_10_1016_j_autrev_2021_102933
crossref_primary_10_1016_j_preteyeres_2012_06_002
crossref_primary_10_1016_j_exer_2023_109679
crossref_primary_10_1097_ICO_0000000000000721
crossref_primary_10_1097_ICL_0000000000000558
crossref_primary_10_1016_j_jtos_2017_03_006
crossref_primary_10_1016_j_exer_2020_108209
crossref_primary_10_3341_kjo_2016_30_4_302
crossref_primary_10_1371_journal_pone_0051979
crossref_primary_10_1080_09273948_2018_1497664
crossref_primary_10_1186_s13063_017_1861_3
crossref_primary_10_3390_bios13070670
crossref_primary_10_3390_cells8060620
crossref_primary_10_1007_s11095_019_2569_8
crossref_primary_10_3389_fonc_2019_00882
crossref_primary_10_1097_ICL_0000000000000042
crossref_primary_10_1016_j_exer_2015_08_024
crossref_primary_10_2174_0929867326666190627131300
crossref_primary_10_1016_j_ophtha_2016_07_028
crossref_primary_10_1016_j_preteyeres_2025_101338
crossref_primary_10_1038_s41598_017_13817_y
crossref_primary_10_1097_MD_0000000000040669
crossref_primary_10_1177_1753425913498912
crossref_primary_10_1016_j_exer_2015_02_013
crossref_primary_10_1080_09273948_2022_2046794
crossref_primary_10_1136_bjophthalmol_2015_307586
ContentType Journal Article
DBID CGR
CUY
CVF
ECM
EIF
NPM
7X8
DOI 10.1167/iovs.11-7266
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList MEDLINE - Academic
MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: 7X8
  name: MEDLINE - Academic
  url: https://search.proquest.com/medline
  sourceTypes: Aggregation Database
DeliveryMethod no_fulltext_linktorsrc
Discipline Medicine
EISSN 1552-5783
ExternalDocumentID 21775656
Genre Research Support, Non-U.S. Gov't
Journal Article
GroupedDBID ---
18M
2WC
34G
39C
5GY
5RE
ACGFO
ACNCT
ADBBV
AENEX
AFOSN
ALMA_UNASSIGNED_HOLDINGS
BAWUL
CGR
CS3
CUY
CVF
DIK
DU5
E3Z
EBS
ECM
EIF
EJD
F5P
GROUPED_DOAJ
GX1
N9A
NPM
OK1
P2P
RHF
RPM
SJN
TR2
TRV
W8F
WH7
WOQ
WOW
7X8
ID FETCH-LOGICAL-c356t-bd55352343eedb69c67ea8ce997fa6b34a6eaf4a244a89691a6f84aad1bdf2a82
IEDL.DBID 7X8
ISICitedReferencesCount 117
ISICitedReferencesURI http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000295467200092&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN 1552-5783
IngestDate Thu Sep 04 16:58:47 EDT 2025
Thu Jan 02 22:06:52 EST 2025
IsPeerReviewed true
IsScholarly true
Issue 10
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c356t-bd55352343eedb69c67ea8ce997fa6b34a6eaf4a244a89691a6f84aad1bdf2a82
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
PMID 21775656
PQID 895857552
PQPubID 23479
ParticipantIDs proquest_miscellaneous_895857552
pubmed_primary_21775656
PublicationCentury 2000
PublicationDate 2011-Sep-29
PublicationDateYYYYMMDD 2011-09-29
PublicationDate_xml – month: 09
  year: 2011
  text: 2011-Sep-29
  day: 29
PublicationDecade 2010
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Investigative ophthalmology & visual science
PublicationTitleAlternate Invest Ophthalmol Vis Sci
PublicationYear 2011
References 22186423 - Invest Ophthalmol Vis Sci. 2011;52(13):9610; author reply 9610-1
References_xml – reference: 22186423 - Invest Ophthalmol Vis Sci. 2011;52(13):9610; author reply 9610-1
SSID ssj0021120
Score 2.4023173
Snippet In the pathogenesis of keratoconjunctivitis sicca, immune processes are thought to play an important role. However, the exact details of the pathomechanisms...
SourceID proquest
pubmed
SourceType Aggregation Database
Index Database
StartPage 7725
SubjectTerms Adolescent
Adult
Aged
Child
Child, Preschool
Dry Eye Syndromes - classification
Dry Eye Syndromes - metabolism
Eye Proteins - metabolism
Female
Humans
Infant
Interferon-gamma - metabolism
Interleukin-1beta - metabolism
Interleukin-6 - metabolism
Male
Middle Aged
Protein Array Analysis
Tears - chemistry
Tears - metabolism
Tumor Necrosis Factor-alpha - metabolism
Young Adult
Title Proinflammatory cytokine profiling of tears from dry eye patients by means of antibody microarrays
URI https://www.ncbi.nlm.nih.gov/pubmed/21775656
https://www.proquest.com/docview/895857552
Volume 52
WOSCitedRecordID wos000295467200092&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV07a8MwEBZtU0qXvh_pCw1dReKHXlMppaFLQoYWshlJliCUWKmdBvzve7KdTIUOXYyxZGN0n3WfdOfvEHqMHI3zhEaEprEhqYwEEVJo4hQLfJw6p0RTbIJPJmI2k9MuN6fq0io3c2IzUefehD3ygZA0FJOk8dPyi4SiUSG42lXQ2EW9BJhMADWfbYMIsLRpVBmDyBgBYCabvHfGB3O_ruCM8LiTR_yVWzY-ZnT8z7c7QUcducTPLRpO0Y4tztDBuAufnyM9LT0gCkCwaILr2NQr_wlNuC3dDW4Me4dDim-Fw48nOIdOtob2Vn61wrrGCwvuLfQDo8y1z-FKyOpTZanq6gJ9jF7fX95IV2SBmISyFdE5DQovSZqAt9RMGsatEsZKycFeOkkVs8qlCmiAEpLJSDEnUqXySOcuViK-RHuFL-w1wkoONXdpULoFFqaHwgF1F_DEyDDFqOsjvBm8DEAcIhOqsP67yrbD10dXrQGyZSu2kcGSiQfSefP3zbfosN3xlSSWd6jn4AO292jfrFfzqnxowAHHyXT8AwrkxpE
linkProvider ProQuest
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Proinflammatory+cytokine+profiling+of+tears+from+dry+eye+patients+by+means+of+antibody+microarrays&rft.jtitle=Investigative+ophthalmology+%26+visual+science&rft.au=Boehm%2C+Nils&rft.au=Riechardt%2C+Aline+I&rft.au=Wiegand%2C+Michaela&rft.au=Pfeiffer%2C+Norbert&rft.date=2011-09-29&rft.eissn=1552-5783&rft.volume=52&rft.issue=10&rft.spage=7725&rft_id=info:doi/10.1167%2Fiovs.11-7266&rft_id=info%3Apmid%2F21775656&rft_id=info%3Apmid%2F21775656&rft.externalDocID=21775656
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1552-5783&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1552-5783&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1552-5783&client=summon