Designed miniaturization of microfluidic biosensor platforms using the stop-flow technique

Here, we present a novel approach to increase the degree of miniaturization as well as the sensitivity of biosensor platforms by the optimization of microfluidic stop-flow techniques independent of the applied detection technique (e.g. electrochemical or optical). The readout of the labeled bioassay...

Celý popis

Uložené v:
Podrobná bibliografia
Vydané v:Analyst (London) Ročník 141; číslo 21; s. 6073
Hlavní autori: Dincer, C, Kling, A, Chatelle, C, Armbrecht, L, Kieninger, J, Weber, W, Urban, G A
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: England 17.10.2016
Predmet:
Biosensing Techniques
Humans
Microfluidic Analytical Techniques
Microfluidics
Miniaturization
Tetracyclines - analysis
Tetracyclines - blood
ISSN:1364-5528, 1364-5528
On-line prístup:Zistit podrobnosti o prístupe
Tagy: Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
Popis
Shrnutí:Here, we present a novel approach to increase the degree of miniaturization as well as the sensitivity of biosensor platforms by the optimization of microfluidic stop-flow techniques independent of the applied detection technique (e.g. electrochemical or optical). The readout of the labeled bioassays, immobilized in a microfluidic channel, under stop-flow conditions leads to a rectangular shaped peak signal. Data evaluation using the peak height allows for a high level miniaturization of the channel geometries. To study the main advantages and limitations of this method by numerical simulations, a universally applicable model system is introduced for the first time. Consequently, proof-of-principle experiments were successfully performed with standard and miniaturized versions of an electrochemical biosensor platform utilizing a repressor protein-based assay for tetracycline antibiotics. Herein, the measured current peak heights are the same despite the sextuple reduction of the channel dimensions. Thus, this results in a 22-fold signal amplification compared to the constant flow measurements in the case of the miniaturized version.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1364-5528
1364-5528
DOI:10.1039/C6AN01330A