Designed miniaturization of microfluidic biosensor platforms using the stop-flow technique

Here, we present a novel approach to increase the degree of miniaturization as well as the sensitivity of biosensor platforms by the optimization of microfluidic stop-flow techniques independent of the applied detection technique (e.g. electrochemical or optical). The readout of the labeled bioassay...

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Bibliographic Details
Published in:Analyst (London) Vol. 141; no. 21; p. 6073
Main Authors: Dincer, C, Kling, A, Chatelle, C, Armbrecht, L, Kieninger, J, Weber, W, Urban, G A
Format: Journal Article
Language:English
Published: England 17.10.2016
Subjects:
Biosensing Techniques
Humans
Microfluidic Analytical Techniques
Microfluidics
Miniaturization
Tetracyclines - analysis
Tetracyclines - blood
ISSN:1364-5528, 1364-5528
Online Access:Get more information
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Summary:Here, we present a novel approach to increase the degree of miniaturization as well as the sensitivity of biosensor platforms by the optimization of microfluidic stop-flow techniques independent of the applied detection technique (e.g. electrochemical or optical). The readout of the labeled bioassays, immobilized in a microfluidic channel, under stop-flow conditions leads to a rectangular shaped peak signal. Data evaluation using the peak height allows for a high level miniaturization of the channel geometries. To study the main advantages and limitations of this method by numerical simulations, a universally applicable model system is introduced for the first time. Consequently, proof-of-principle experiments were successfully performed with standard and miniaturized versions of an electrochemical biosensor platform utilizing a repressor protein-based assay for tetracycline antibiotics. Herein, the measured current peak heights are the same despite the sextuple reduction of the channel dimensions. Thus, this results in a 22-fold signal amplification compared to the constant flow measurements in the case of the miniaturized version.
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ISSN:1364-5528
1364-5528
DOI:10.1039/C6AN01330A