Designed miniaturization of microfluidic biosensor platforms using the stop-flow technique
Here, we present a novel approach to increase the degree of miniaturization as well as the sensitivity of biosensor platforms by the optimization of microfluidic stop-flow techniques independent of the applied detection technique (e.g. electrochemical or optical). The readout of the labeled bioassay...
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| Veröffentlicht in: | Analyst (London) Jg. 141; H. 21; S. 6073 |
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| Sprache: | Englisch |
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17.10.2016
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| Abstract | Here, we present a novel approach to increase the degree of miniaturization as well as the sensitivity of biosensor platforms by the optimization of microfluidic stop-flow techniques independent of the applied detection technique (e.g. electrochemical or optical). The readout of the labeled bioassays, immobilized in a microfluidic channel, under stop-flow conditions leads to a rectangular shaped peak signal. Data evaluation using the peak height allows for a high level miniaturization of the channel geometries. To study the main advantages and limitations of this method by numerical simulations, a universally applicable model system is introduced for the first time. Consequently, proof-of-principle experiments were successfully performed with standard and miniaturized versions of an electrochemical biosensor platform utilizing a repressor protein-based assay for tetracycline antibiotics. Herein, the measured current peak heights are the same despite the sextuple reduction of the channel dimensions. Thus, this results in a 22-fold signal amplification compared to the constant flow measurements in the case of the miniaturized version. |
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| AbstractList | Here, we present a novel approach to increase the degree of miniaturization as well as the sensitivity of biosensor platforms by the optimization of microfluidic stop-flow techniques independent of the applied detection technique (e.g. electrochemical or optical). The readout of the labeled bioassays, immobilized in a microfluidic channel, under stop-flow conditions leads to a rectangular shaped peak signal. Data evaluation using the peak height allows for a high level miniaturization of the channel geometries. To study the main advantages and limitations of this method by numerical simulations, a universally applicable model system is introduced for the first time. Consequently, proof-of-principle experiments were successfully performed with standard and miniaturized versions of an electrochemical biosensor platform utilizing a repressor protein-based assay for tetracycline antibiotics. Herein, the measured current peak heights are the same despite the sextuple reduction of the channel dimensions. Thus, this results in a 22-fold signal amplification compared to the constant flow measurements in the case of the miniaturized version.Here, we present a novel approach to increase the degree of miniaturization as well as the sensitivity of biosensor platforms by the optimization of microfluidic stop-flow techniques independent of the applied detection technique (e.g. electrochemical or optical). The readout of the labeled bioassays, immobilized in a microfluidic channel, under stop-flow conditions leads to a rectangular shaped peak signal. Data evaluation using the peak height allows for a high level miniaturization of the channel geometries. To study the main advantages and limitations of this method by numerical simulations, a universally applicable model system is introduced for the first time. Consequently, proof-of-principle experiments were successfully performed with standard and miniaturized versions of an electrochemical biosensor platform utilizing a repressor protein-based assay for tetracycline antibiotics. Herein, the measured current peak heights are the same despite the sextuple reduction of the channel dimensions. Thus, this results in a 22-fold signal amplification compared to the constant flow measurements in the case of the miniaturized version. Here, we present a novel approach to increase the degree of miniaturization as well as the sensitivity of biosensor platforms by the optimization of microfluidic stop-flow techniques independent of the applied detection technique (e.g. electrochemical or optical). The readout of the labeled bioassays, immobilized in a microfluidic channel, under stop-flow conditions leads to a rectangular shaped peak signal. Data evaluation using the peak height allows for a high level miniaturization of the channel geometries. To study the main advantages and limitations of this method by numerical simulations, a universally applicable model system is introduced for the first time. Consequently, proof-of-principle experiments were successfully performed with standard and miniaturized versions of an electrochemical biosensor platform utilizing a repressor protein-based assay for tetracycline antibiotics. Herein, the measured current peak heights are the same despite the sextuple reduction of the channel dimensions. Thus, this results in a 22-fold signal amplification compared to the constant flow measurements in the case of the miniaturized version. |
| Author | Dincer, C Armbrecht, L Kieninger, J Weber, W Urban, G A Kling, A Chatelle, C |
| Author_xml | – sequence: 1 givenname: C orcidid: 0000-0003-3301-1198 surname: Dincer fullname: Dincer, C email: dincer@imtek.de organization: Laboratory for Sensors, Department of Microsystems Engineering - IMTEK, University of Freiburg, Germany. dincer@imtek.de and Freiburg Materials Research Center - FMF, University of Freiburg, Germany – sequence: 2 givenname: A surname: Kling fullname: Kling, A email: dincer@imtek.de organization: Laboratory for Sensors, Department of Microsystems Engineering - IMTEK, University of Freiburg, Germany. dincer@imtek.de – sequence: 3 givenname: C surname: Chatelle fullname: Chatelle, C organization: Centre for Biological Signalling Studies - BIOSS, Germany and Faculty of Biology, University of Freiburg, Germany – sequence: 4 givenname: L surname: Armbrecht fullname: Armbrecht, L email: dincer@imtek.de organization: Laboratory for Sensors, Department of Microsystems Engineering - IMTEK, University of Freiburg, Germany. dincer@imtek.de – sequence: 5 givenname: J surname: Kieninger fullname: Kieninger, J email: dincer@imtek.de organization: Laboratory for Sensors, Department of Microsystems Engineering - IMTEK, University of Freiburg, Germany. dincer@imtek.de – sequence: 6 givenname: W surname: Weber fullname: Weber, W organization: Centre for Biological Signalling Studies - BIOSS, Germany and Faculty of Biology, University of Freiburg, Germany and Spemann School of Biology and Medicine, University of Freiburg, Germany – sequence: 7 givenname: G A surname: Urban fullname: Urban, G A email: dincer@imtek.de organization: Laboratory for Sensors, Department of Microsystems Engineering - IMTEK, University of Freiburg, Germany. dincer@imtek.de and Freiburg Materials Research Center - FMF, University of Freiburg, Germany |
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| SubjectTerms | Biosensing Techniques Humans Microfluidic Analytical Techniques Microfluidics Miniaturization Tetracyclines - analysis Tetracyclines - blood |
| Title | Designed miniaturization of microfluidic biosensor platforms using the stop-flow technique |
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