Structure-activity study of LVV-hemorphin-7: angiotensin AT4 receptor ligand and inhibitor of insulin-regulated aminopeptidase

The decapeptide LVV-hemorphin-7 binds with high affinity to the angiotensin IV (Ang IV) receptor (AT(4) receptor), eliciting a number of physiological effects, including cellular proliferation and memory enhancement. We have recently shown that the AT(4) receptor is identical to insulin-regulated am...

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Vydáno v:The Journal of pharmacology and experimental therapeutics Ročník 305; číslo 1; s. 205
Hlavní autoři: Lee, Joohyung, Mustafa, Tomris, McDowall, Sharon G, Mendelsohn, Frederick A O, Brennan, Michelle, Lew, Rebecca A, Albiston, Anthony L, Chai, Siew Yeen
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 01.04.2003
Témata:
Alanine - genetics
Alanine - metabolism
Amino Acid Motifs
Amino Acid Substitution
Aminopeptidases - metabolism
Angiotensin Receptor Antagonists
Cells, Cultured
Drug Interactions
Gene Deletion
Hemoglobins - chemistry
Hemoglobins - genetics
Hemoglobins - pharmacology
Humans
Insulin - pharmacology
Interleukin 1 Receptor Antagonist Protein
Ligands
Peptide Fragments - chemistry
Peptide Fragments - genetics
Peptide Fragments - pharmacology
Peptides - pharmacology
Receptors, Angiotensin
Recombinant Proteins - metabolism
Sialoglycoproteins - metabolism
Structure-Activity Relationship
ISSN:0022-3565
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Shrnutí:The decapeptide LVV-hemorphin-7 binds with high affinity to the angiotensin IV (Ang IV) receptor (AT(4) receptor), eliciting a number of physiological effects, including cellular proliferation and memory enhancement. We have recently shown that the AT(4) receptor is identical to insulin-regulated aminopeptidase (IRAP) and that both LVV-hemorphin-7 and Ang IV inhibit the catalytic activity of IRAP. In the current study, a series of alanine-substituted and N- or C-terminally modified analogs of LVV-hemorphin-7 were evaluated for their abilities to compete for (125)I-Ang IV binding in sheep adrenal and cerebellar membranes. Selected analogs were also analyzed for binding to recombinant human IRAP and inhibition of IRAP aminopeptidase activity. C-Terminal deletions of LVV-hemorphin-7 resulted in modest changes in affinity for IRAP, whereas deletion of the first three N-terminal residues abolished binding. Monosubstitutions of Tyr(4) and Trp(6) with alanine resulted in a 10-fold reduction in affinity. Competition binding studies using recombinant human IRAP demonstrated the same rank order of affinity as obtained for the ovine tissues. All LVV-hemorphin-7 analogs tested, except for Leu-Val-Val-Tyr, inhibit the cleavage of the synthetic substrate, leucine beta-naphthylamide, by IRAP, with K(i) values between 56 and 620 nM. We find that the Val(3) residue is crucial for LVV-hemorphin-7 binding to IRAP, whereas the C-terminal domain seems to play a minor role. The current study highlights the minimal residues necessary for binding and inhibition of IRAP and provides a basis to design peptidomimetic analogs for experimental and potentially clinical use.
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ISSN:0022-3565
DOI:10.1124/jpet.102.045492