PtHAK5, a candidate for mediating high-affinity K + uptake in the halophytic grass, Puccinellia tenuiflora

Puccinellia tenuiflora is a typical salt-exclu-ding halophytic grass with strong salt-tolerance, which enhances tolerance by restricting Na + influx as well as having a strong selectivity for K + over Na +. The HAK5 K + transporters generally modulate effective K + acquisition in plants, especially...

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Published in:	Frontiers of Agricultural Science and Engineering Vol. 5; no. 1; pp. 108 - 117
Main Authors:	YANG, Haili, ZHANG, Weidan, CHAI, Weiwei, WANG, Wenying, GAO, Li, ZHANG, Jing, WANG, Yongping, WANG, Suo-Min
Format:	Journal Article
Language:	English
Published:	Higher Education Press 01.03.2018
Subjects:	K + uptake K+ uptake\|PtHAK5\|Puccinellia tenuiflora\|yeast complementation PtHAK5 Puccinellia tenuiflora yeast complementation
ISSN:	2095-7505, 2095-977X
Online Access:	Get full text
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Summary:	Puccinellia tenuiflora is a typical salt-exclu-ding halophytic grass with strong salt-tolerance, which enhances tolerance by restricting Na + influx as well as having a strong selectivity for K + over Na +. The HAK5 K + transporters generally modulate effective K + acquisition in plants, especially under low K + condition. In this study, PtHAK5 from P. tenuiflora was isolated by RT-PCR and characterized using yeast complementation. The results showed PtHAK5 consisted of 784 amino acids and shared over 80% homology with the identified high-affinity K + transporter HAK5 from other higher plants. The expression of PtHAK5 rescued the K +-uptake-defective phenotype of yeast strain CY162. In conclusion, PtHAK5 is a candidate for mediating high-affinity K + uptake under low K + conditions.
Bibliography:	yeast complementation Puccinellia tenuiflora Document received on :2017-09-03 Document accepted on :2017-11-12 K + uptake PtHAK5
ISSN:	2095-7505 2095-977X
DOI:	10.15302/J-FASE-2018200