Development of a Silver-Based MOF Oxidase-Like nanozyme modified with molecularly imprinted polymer for sensitive and selective colorimetric detection of quercetin

[Display omitted] •A new Ag-MOF with oxidase-like activity is developed.•The Ag-MOF nanozyme exhibits a catalytic efficiency of 0.19 µmol min−1 mg−1.•The integration of MIP onto Ag-MOF ensures selective detection of quercetin.•The Ag-MOF@MIP nanozyme detects quercetin with a LOD of 0.43 μM. Antioxid...

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Published in:Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 330; p. 125735
Main Authors: Karrat, Abdelhafid, Benssbihe, Jihane, Mohammad Ameen, Sameera Sh, Omer, Khalid M., Amine, Aziz
Format: Journal Article
Language:English
Published: England Elsevier B.V 05.04.2025
Subjects:
Antioxidants detection
Colorimetric detection
Colorimetry - methods
Limit of Detection
Metal-Organic Frameworks - chemistry
Molecular Imprinting
Molecularly Imprinted Polymers - chemistry
Oxidoreductases - chemistry
Quercetin - analysis
Quercetin, MOF@MIP oxidase-like nanozyme
Silver - chemistry
Antioxidants detection
Quercetin, MOF@MIP oxidase-like nanozyme
Colorimetric detection
ISSN:1386-1425, 1873-3557, 1873-3557
Online Access:Get full text
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Summary:[Display omitted] •A new Ag-MOF with oxidase-like activity is developed.•The Ag-MOF nanozyme exhibits a catalytic efficiency of 0.19 µmol min−1 mg−1.•The integration of MIP onto Ag-MOF ensures selective detection of quercetin.•The Ag-MOF@MIP nanozyme detects quercetin with a LOD of 0.43 μM. Antioxidants are vital components in various food, plant, and pharmacological products, making their quantitative, selective, and straightforward assessment essential for evaluating product quality and health benefits. Nanozymes, such as metal–organic frameworks (MOFs) with enzyme-like catalytic activity, hold significant potential for developing highly efficient antioxidant sensing platforms. This is due to their large specific surface area, low density, high porosity, structural diversity, and adjustable pore size. In this study, we synthesized a silver-based MOF exhibiting effective oxidase-like activity and modified it with molecularly imprinted polymer (MIP) using radical polymerization. The developed system (Ag-MOF@MIP) was successfully applied for the colorimetric detection of quercetin, achieving a limit of detection of 0.43 μM and a limit of quantification of 1 μM within a detection range of 1 to 132 μM. This combination exhibited improved selectivity and sensitivity towards quercetin and demonstrated high stability after one month of storage. This detection strategy can also be used to detect other analytes using TMB as a probe.
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ISSN:1386-1425
1873-3557
1873-3557
DOI:10.1016/j.saa.2025.125735