Analysis of proximal bone margins in diabetic foot osteomyelitis by conventional culture, DNA sequencing and microscopy

Multiple approaches were employed to detect pathogens from bone margins associated with Diabetic Foot Osteomyelitis (DFO). Intra‐operative bone specimens of 14 consecutive subjects with suspected DFO were collected over a six‐month study period from Liverpool Hospital. Infected bone and a proximal b...

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Bibliographic Details
Published in:	APMIS : acta pathologica, microbiologica et immunologica Scandinavica Vol. 127; no. 10; pp. 660 - 670
Main Authors:	Malone, Matthew, Fritz, Blaine G., Vickery, Karen, Schwarzer, Saskia, Sharma, Varun, Biggs, Nathan, Radzieta, Michael, Jeffries, Thomas T., Dickson, Hugh G., Jensen, Slade O., Bjarnsholt, Thomas
Format:	Journal Article
Language:	English
Published:	Denmark Wiley Subscription Services, Inc 01.10.2019
Subjects:	16S rRNA Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Bacteriological Techniques - methods Biomedical materials Bone and Bones - microbiology Bone and Bones - surgery Bone growth Culture Deoxyribonucleic acid Diabetes Diabetes mellitus diabetic foot Diabetic Foot - microbiology Diabetic Foot - pathology Diabetic Foot - surgery DNA DNA sequencing Feet Foot diseases Histocytochemistry - methods Humans In Situ Hybridization, Fluorescence Metagenomics - methods Microbiomes Microorganisms Microscopy Microscopy, Electron, Scanning Osteomyelitis Osteomyelitis - microbiology Osteomyelitis - pathology Osteomyelitis - surgery Pathogens Sequence Analysis, DNA Statistical analysis surgical management Osteomyelitis 16S rRNA surgical management diabetic foot
ISSN:	0903-4641, 1600-0463, 1600-0463
Online Access:	Get full text
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