Study of IL4‐590C/T and IL6‐174G/C Gene Polymorphisms in Type 2 Diabetic Patients With Chronic Kidney Disease in North Indian Population

ABSTRACT To explore the associations between potential functional promoter polymorphisms in pro‐inflammatory and anti‐inflammatory (IL‐4(‐590C/T) and IL‐6(‐174G/C) cytokine genes, and kidney dysfunction in North Indian type 2 diabetic subjects with chronic kidney disease. A total of 150 subjects age...

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Published in:Journal of cellular biochemistry Vol. 118; no. 7; pp. 1803 - 1809
Main Authors: Neelofar, Km, Ahmad, Jamal, Ahmad, Arif, Alam, Khursheed
Format: Journal Article
Language:English
Published: United States Wiley Subscription Services, Inc 01.07.2017
Subjects:
Adult
Aged
Asian Continental Ancestry Group
C gene
CKD
Creatinine
Creatinine - blood
Diabetes
Diabetes mellitus
Diabetes Mellitus, Type 2
Electrophoresis, Agar Gel
Female
Gene Frequency - genetics
GENE POLYMORPHISM
Genes
Genetic Association Studies
Genetic Predisposition to Disease - genetics
Genotype
Genotypes
Glomerular filtration rate
Glomerular Filtration Rate - genetics
Glomerular Filtration Rate - physiology
Humans
IL‐4
IL‐6
Inflammation
Interleukin 4
Interleukin 6
Interleukin-4 - genetics
Interleukin-6 - genetics
Kidney diseases
Kidney transplantation
Kidneys
Male
Middle Aged
Polymerase chain reaction
Polymorphism, Single Nucleotide - genetics
Promoter Regions, Genetic - genetics
Renal Insufficiency, Chronic - blood
Renal Insufficiency, Chronic - genetics
TYPE 2 DIABETES
IL-4
TYPE 2 DIABETES
CKD
GENE POLYMORPHISM
IL-6
ISSN:0730-2312, 1097-4644, 1097-4644
Online Access:Get full text
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Summary:ABSTRACT To explore the associations between potential functional promoter polymorphisms in pro‐inflammatory and anti‐inflammatory (IL‐4(‐590C/T) and IL‐6(‐174G/C) cytokine genes, and kidney dysfunction in North Indian type 2 diabetic subjects with chronic kidney disease. A total of 150 subjects aged 25–75 year were included in this study. The glomerular filtration rate (GFR) and serum creatinine were estimated. PCR was performed to analyse genotype distribution in IL‐4 (‐590T/C) and IL‐6 (‐174G/C) among healthy, type 2 diabetic patients with or without CKD. The genotype distributions were determined by Hardy–Weinberg equilibrium. CKD patients showed lower GFR (59.36 ± 1.33 ml/min/1.73 m2) and higher serum creatinine (1.93 ± 0.99% mg) level in comparison to diabetic patients without CKD and healthy subjects. Genotypic distribution of the different genotypes among the study groups in IL‐4 gene was genotype CC = 30, TC = 12, and TT = 8 in CKD patients. In type 2 diabetic patients without CKD, genotype distribution was CC = 38, TC = 10, and TT = 2. In healthy subjects, distribution of genotype was CC = 35, TC = 14, and TT = 1. The distribution of different genotype among the study groups for IL‐6 gene was GG = 27, GC = 20, and CC = 3 in healthy subjects; GG = 28, GC = 19, and CC = 3 in diabetic patients without CKD and GG = 38, GC = 11, and CC = 1 in diabetic patients with CKD. There was no significant difference in the distribution of genotype frequencies between healthy subjects and diabetic patients without CKD but a significant difference was found in diabetic patients with CKD. The functional promoter polymorphisms IL4‐590C/T and IL6‐174G/C, which affect the IL‐4 and IL‐6 levels in north Indian subjects, were associated with kidney dysfunction and CKD. J. Cell. Biochem. 118: 1803–1809, 2017. © 2016 Wiley Periodicals, Inc. The associations between common potential functional promoter polymorphisms in pro‐inflammatory and anti‐inflammatory (IL‐4(‐590C/T) and IL‐6(‐174G/C) cytokine genes and kidney dysfunction in north Indian type 2 diabetic patients with chronic kidney disease.
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ISSN:0730-2312
1097-4644
1097-4644
DOI:10.1002/jcb.25853