Transient Sub-cellular Localization and In Vivo Protein-Protein Interaction Study of Multiple Abiotic Stress-Responsive AteIF4A-III and AtALY4 Proteins in Arabidopsis thaliana

Major abiotic stress factors such as drought, salinity, hypoxia, and extreme temperatures along with rapid global climate change have had a huge negative impact on agricultural productivity. Understanding the abiotic stress-responsive molecular mechanisms and its associated proteins is extremely imp...

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Bibliographic Details
Published in:Plant molecular biology reporter Vol. 38; no. 4; pp. 538 - 553
Main Authors: Baruah, Indrani, Baruah, Geetanjali, Sahu, Jagajjit, Singha, Dhanawantari L., Dekaboruah, Hariprasanna, Velmurugan, Natarajan, Chikkaputtaiah, Channakeshavaiah
Format: Journal Article
Language:English
Published: New York Springer US 01.12.2020
Springer Nature B.V
Subjects:
Abiotic factors
Abiotic stress
Agricultural production
agricultural productivity
Arabidopsis thaliana
Bioinformatics
Biomedical and Life Sciences
Climate change
Cytoplasm
DEAD-box RNA helicases
DNA helicase
Drought
Flow cytometry
Global climate
Hypoxia
In vivo methods and tests
Life Sciences
Localization
Metabolomics
molecular biology
Molecular modelling
mRNA
Nuclei (cytology)
Original Paper
Plant Breeding/Biotechnology
Plant Sciences
Protein interaction
protein-protein interactions
Proteins
Proteomics
protoplasts
RNA helicase
RNA transport
salinity
stress response
stress tolerance
Stresses
Strong interactions (field theory)
DEAD-box RNA helicases
Re-localization
Protein-protein interaction
AtALY4
Multiple abiotic stresses
AteIF4A-III
ISSN:0735-9640, 1572-9818
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Summary:Major abiotic stress factors such as drought, salinity, hypoxia, and extreme temperatures along with rapid global climate change have had a huge negative impact on agricultural productivity. Understanding the abiotic stress-responsive molecular mechanisms and its associated proteins is extremely important to advance our knowledge towards developing multiple abiotic stress tolerance in plants. Firstly, basic understanding at transient level would be a vital foundation to accomplish this goal. Therefore, our present study aimed at understanding the sub-cellular localization of Eukaryotic Initiation Factor 4A-III (AteIF4A-III), a key DEAD-box RNA helicase, and Always Early 4 (AtALY4), an mRNA export factor, and their in vivo protein-protein interaction with major abiotic stress–associated proteins under control and multiple abiotic stress conditions. AteIF4A-III and AtALY4 were localized to the nucleus as evident by transient protoplast assay. AteIF4A-III has shown strong interaction with a negative regulator of multiple abiotic stresses, Stress Response Suppressor 1 (AtSTRS1) in Bi-FC assay. Further, the flow cytometry analysis has shown the strong interaction between them. Interestingly, under multiple abiotic stress treatment, the interacting partners were rapidly re-localized from nucleus to cytoplasm and cytoplasmic space. Similar results were observed when N- and C-terminal fusions of AteIF4A-III and AtALY4 treated under multiple abiotic stresses. Our study reveals that AteIF4A-III, AtALY4, and abiotic stress–associated protein AtSTRS1 are among the key proteins associated with multiple abiotic stress responses in plants.
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ISSN:0735-9640
1572-9818
DOI:10.1007/s11105-020-01219-w