Transcriptomes of human primary skin fibroblasts of healthy individuals reveal age‐associated mRNAs and long noncoding RNAs

Changes in the transcriptomes of human tissues with advancing age are poorly cataloged. Here, we sought to identify the coding and long noncoding RNAs present in cultured primary skin fibroblasts collected from 82 healthy individuals across a wide age spectrum (22–89 years old) who participated in t...

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Published in:Aging cell Vol. 22; no. 11; p. e13915
Main Authors: Tsitsipatis, Dimitrios, Martindale, Jennifer L., Mazan‐Mamczarz, Krystyna, Herman, Allison B., Piao, Yulan, Banskota, Nirad, Yang, Jen‐Hao, Cui, Linna, Anerillas, Carlos, Chang, Ming‐Wen, Kaileh, Mary, Munk, Rachel, Yang, Xiaoling, Ubaida‐Mohien, Ceereena, Chia, Chee W., Karikkineth, Ajoy C., Zukley, Linda, D'Agostino, Jarod, Abdelmohsen, Kotb, Basisty, Nathan, De, Supriyo, Ferrucci, Luigi, Gorospe, Myriam
Format: Journal Article
Language:English
Published: England John Wiley & Sons, Inc 01.11.2023
Subjects:
Adult
Age
Aged
Aged, 80 and over
Aging
Biomarkers - metabolism
Biopsy
Cytokines
Dehydrogenases
Epigenetics
Extracellular matrix
Fibroblasts
Fibroblasts - metabolism
Gene Expression Profiling
Gene set enrichment analysis
Growth factors
Humans
Hyaluronic acid
Kinases
Males
Middle Aged
Non-coding RNA
Proteins
Regression analysis
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA-binding protein
Skin
Therapeutic targets
Transcription factors
Transcriptome - genetics
Transcriptomes
White people
Young Adult
messenger RNAs
long noncoding RNAs
aging
human dermal fibroblasts
circular RNAs
transcriptome
ISSN:1474-9718, 1474-9726, 1474-9726
Online Access:Get full text
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Summary:Changes in the transcriptomes of human tissues with advancing age are poorly cataloged. Here, we sought to identify the coding and long noncoding RNAs present in cultured primary skin fibroblasts collected from 82 healthy individuals across a wide age spectrum (22–89 years old) who participated in the GESTALT (Genetic and Epigenetic Signatures of Translational Aging Laboratory Testing) study of the National Institute on Aging, NIH. Using high‐throughput RNA sequencing and a linear regression model, we identified 1437 coding RNAs (mRNAs) and 1177 linear and circular long noncoding (lncRNAs) that were differentially abundant as a function of age. Gene set enrichment analysis (GSEA) revealed select transcription factors implicated in coordinating the transcription of subsets of differentially abundant mRNAs, while long noncoding RNA enrichment analysis (LncSEA) identified RNA‐binding proteins predicted to participate in the age‐associated lncRNA profiles. In summary, we report age‐associated changes in the global transcriptome, coding and noncoding, from healthy human skin fibroblasts and propose that these transcripts may serve as biomarkers and therapeutic targets in aging skin.
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ISSN:1474-9718
1474-9726
1474-9726
DOI:10.1111/acel.13915