A self-assembled multi-enzyme nanomachine and whole-cell biocatalyst for the sustainable valorization of marine biomass into fermentable sugars and consolidated bioprocessing

Marine biomass is a potential resource that contains functional carbohydrates, such as agar, carrageenan, and cellulose, and can be modified for various applications. A novel self-assembled multi-enzyme nanomachine (SAMN) and its whole-cell biocatalyst were developed to target the degradation of mar...

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Vydáno v:	International journal of biological macromolecules Ročník 322; číslo Pt 3; s. 146853
Hlavní autoři:	Lee, Myeong-Eun, Bhardwaj, Nisha, Cho, Byeong-Hyeon, Hyeon, Jeong Eun, Jeong, Wu-Young, Han, Sung Ok
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Netherlands Elsevier B.V 01.09.2025
Témata:	Agar - chemistry Aquatic Organisms Biocatalysis Biomass Biomass-to-biofuel conversion Carbohydrate Metabolism Carrageenan - chemistry Cellulose - chemistry Fermentation Hydrolysis Marine carbohydrate-active enzymes Marine polysaccharide Modular enzyme assembly Rhodophyta - chemistry Saccharomyces cerevisiae - metabolism Sugars - metabolism Surface-engineered microbial catalyst Marine polysaccharide Modular enzyme assembly Biomass-to-biofuel conversion Marine carbohydrate-active enzymes Surface-engineered microbial catalyst
ISSN:	0141-8130, 1879-0003, 1879-0003
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Abstract	Marine biomass is a potential resource that contains functional carbohydrates, such as agar, carrageenan, and cellulose, and can be modified for various applications. A novel self-assembled multi-enzyme nanomachine (SAMN) and its whole-cell biocatalyst were developed to target the degradation of marine biomass components and enable consolidated bioprocessing. Compared to each representative single enzyme, the marine biomass-degrading SAMN exhibited an average 1.45-fold higher hydrolytic activity for six of the seven substrates. The modification of SAMN with MglB further enhanced the hydrolytic activities toward agar, agarose, lambda-carrageenan, and agar-carrageenans-cellulose mixed substrate. Moreover, SAMN displayed a maximum thermostability increase of 14.10%. The SAMN and the SAMN with MglB produced a maximum of 2.25 ± 0.04 mM and 2.33 ± 0.03 mM galactose, a functional sugar from real red algae, respectively. The Saccharomyces cerevisiae-based whole-cell biocatalyst displaying SAMN with MglB produced 2.00 ± 0.14 g/L ethanol from real red algae as the sole carbon source, achieving the highest production among all samples. This marine biomass-degrading SAMN and its whole-cell biocatalyst have potential applications in the saccharification and consolidated bioprocessing of marine biomass, leading to the production of functional sugars and value-added bio-based products in industrial settings. [Display omitted] •Self-assembled multi-enzyme nanomachine (SAMN) was newly developed.•SAMN showed enhanced hydrolysis activity toward marine biomass-related substrates.•SAMN was thermostable in the condition of high temperature.•The modified SAMN produced maximum 2.33 ± 0.03 mM galactose from real red algae.•The SAMN-displaying whole-cell biocatalyst produced 2.00 ± 0.14 g/L ethanol from real red algae.
AbstractList	Marine biomass is a potential resource that contains functional carbohydrates, such as agar, carrageenan, and cellulose, and can be modified for various applications. A novel self-assembled multi-enzyme nanomachine (SAMN) and its whole-cell biocatalyst were developed to target the degradation of marine biomass components and enable consolidated bioprocessing. Compared to each representative single enzyme, the marine biomass-degrading SAMN exhibited an average 1.45-fold higher hydrolytic activity for six of the seven substrates. The modification of SAMN with MglB further enhanced the hydrolytic activities toward agar, agarose, lambda-carrageenan, and agar-carrageenans-cellulose mixed substrate. Moreover, SAMN displayed a maximum thermostability increase of 14.10%. The SAMN and the SAMN with MglB produced a maximum of 2.25 ± 0.04 mM and 2.33 ± 0.03 mM galactose, a functional sugar from real red algae, respectively. The Saccharomyces cerevisiae-based whole-cell biocatalyst displaying SAMN with MglB produced 2.00 ± 0.14 g/L ethanol from real red algae as the sole carbon source, achieving the highest production among all samples. This marine biomass-degrading SAMN and its whole-cell biocatalyst have potential applications in the saccharification and consolidated bioprocessing of marine biomass, leading to the production of functional sugars and value-added bio-based products in industrial settings.Marine biomass is a potential resource that contains functional carbohydrates, such as agar, carrageenan, and cellulose, and can be modified for various applications. A novel self-assembled multi-enzyme nanomachine (SAMN) and its whole-cell biocatalyst were developed to target the degradation of marine biomass components and enable consolidated bioprocessing. Compared to each representative single enzyme, the marine biomass-degrading SAMN exhibited an average 1.45-fold higher hydrolytic activity for six of the seven substrates. The modification of SAMN with MglB further enhanced the hydrolytic activities toward agar, agarose, lambda-carrageenan, and agar-carrageenans-cellulose mixed substrate. Moreover, SAMN displayed a maximum thermostability increase of 14.10%. The SAMN and the SAMN with MglB produced a maximum of 2.25 ± 0.04 mM and 2.33 ± 0.03 mM galactose, a functional sugar from real red algae, respectively. The Saccharomyces cerevisiae-based whole-cell biocatalyst displaying SAMN with MglB produced 2.00 ± 0.14 g/L ethanol from real red algae as the sole carbon source, achieving the highest production among all samples. This marine biomass-degrading SAMN and its whole-cell biocatalyst have potential applications in the saccharification and consolidated bioprocessing of marine biomass, leading to the production of functional sugars and value-added bio-based products in industrial settings. Marine biomass is a potential resource that contains functional carbohydrates, such as agar, carrageenan, and cellulose, and can be modified for various applications. A novel self-assembled multi-enzyme nanomachine (SAMN) and its whole-cell biocatalyst were developed to target the degradation of marine biomass components and enable consolidated bioprocessing. Compared to each representative single enzyme, the marine biomass-degrading SAMN exhibited an average 1.45-fold higher hydrolytic activity for six of the seven substrates. The modification of SAMN with MglB further enhanced the hydrolytic activities toward agar, agarose, lambda-carrageenan, and agar-carrageenans-cellulose mixed substrate. Moreover, SAMN displayed a maximum thermostability increase of 14.10%. The SAMN and the SAMN with MglB produced a maximum of 2.25 ± 0.04 mM and 2.33 ± 0.03 mM galactose, a functional sugar from real red algae, respectively. The Saccharomyces cerevisiae-based whole-cell biocatalyst displaying SAMN with MglB produced 2.00 ± 0.14 g/L ethanol from real red algae as the sole carbon source, achieving the highest production among all samples. This marine biomass-degrading SAMN and its whole-cell biocatalyst have potential applications in the saccharification and consolidated bioprocessing of marine biomass, leading to the production of functional sugars and value-added bio-based products in industrial settings. [Display omitted] •Self-assembled multi-enzyme nanomachine (SAMN) was newly developed.•SAMN showed enhanced hydrolysis activity toward marine biomass-related substrates.•SAMN was thermostable in the condition of high temperature.•The modified SAMN produced maximum 2.33 ± 0.03 mM galactose from real red algae.•The SAMN-displaying whole-cell biocatalyst produced 2.00 ± 0.14 g/L ethanol from real red algae. Marine biomass is a potential resource that contains functional carbohydrates, such as agar, carrageenan, and cellulose, and can be modified for various applications. A novel self-assembled multi-enzyme nanomachine (SAMN) and its whole-cell biocatalyst were developed to target the degradation of marine biomass components and enable consolidated bioprocessing. Compared to each representative single enzyme, the marine biomass-degrading SAMN exhibited an average 1.45-fold higher hydrolytic activity for six of the seven substrates. The modification of SAMN with MglB further enhanced the hydrolytic activities toward agar, agarose, lambda-carrageenan, and agar-carrageenans-cellulose mixed substrate. Moreover, SAMN displayed a maximum thermostability increase of 14.10%. The SAMN and the SAMN with MglB produced a maximum of 2.25 ± 0.04 mM and 2.33 ± 0.03 mM galactose, a functional sugar from real red algae, respectively. The Saccharomyces cerevisiae-based whole-cell biocatalyst displaying SAMN with MglB produced 2.00 ± 0.14 g/L ethanol from real red algae as the sole carbon source, achieving the highest production among all samples. This marine biomass-degrading SAMN and its whole-cell biocatalyst have potential applications in the saccharification and consolidated bioprocessing of marine biomass, leading to the production of functional sugars and value-added bio-based products in industrial settings.
ArticleNumber	146853
Author	Bhardwaj, Nisha Lee, Myeong-Eun Hyeon, Jeong Eun Jeong, Wu-Young Han, Sung Ok Cho, Byeong-Hyeon
Author_xml	– sequence: 1 givenname: Myeong-Eun surname: Lee fullname: Lee, Myeong-Eun organization: Department of Biotechnology, Korea University, Seoul 02841, Republic of Korea – sequence: 2 givenname: Nisha surname: Bhardwaj fullname: Bhardwaj, Nisha organization: Department of Biotechnology, Korea University, Seoul 02841, Republic of Korea – sequence: 3 givenname: Byeong-Hyeon surname: Cho fullname: Cho, Byeong-Hyeon organization: Department of Biotechnology, Korea University, Seoul 02841, Republic of Korea – sequence: 4 givenname: Jeong Eun surname: Hyeon fullname: Hyeon, Jeong Eun organization: Department of Food Science and Biotechnology, College of Knowledge-Based Services Engineering, Sungshin Women's University, Seoul 01133, Republic of Korea – sequence: 5 givenname: Wu-Young surname: Jeong fullname: Jeong, Wu-Young organization: Department of Biotechnology, Korea University, Seoul 02841, Republic of Korea – sequence: 6 givenname: Sung Ok surname: Han fullname: Han, Sung Ok email: samhan@korea.ac.kr organization: Department of Biotechnology, Korea University, Seoul 02841, Republic of Korea
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Keywords	Marine polysaccharide Modular enzyme assembly Biomass-to-biofuel conversion Marine carbohydrate-active enzymes Surface-engineered microbial catalyst
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Snippet	Marine biomass is a potential resource that contains functional carbohydrates, such as agar, carrageenan, and cellulose, and can be modified for various...
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SubjectTerms	Agar - chemistry Aquatic Organisms Biocatalysis Biomass Biomass-to-biofuel conversion Carbohydrate Metabolism Carrageenan - chemistry Cellulose - chemistry Fermentation Hydrolysis Marine carbohydrate-active enzymes Marine polysaccharide Modular enzyme assembly Rhodophyta - chemistry Saccharomyces cerevisiae - metabolism Sugars - metabolism Surface-engineered microbial catalyst
Title	A self-assembled multi-enzyme nanomachine and whole-cell biocatalyst for the sustainable valorization of marine biomass into fermentable sugars and consolidated bioprocessing
URI	https://dx.doi.org/10.1016/j.ijbiomac.2025.146853 https://www.ncbi.nlm.nih.gov/pubmed/40812633 https://www.proquest.com/docview/3239785193
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